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        Identification of Green Alga Chlorella vulgaris Isolated from Freshwater and Improvement Biodiesel Productivity via UV Irradiation

        ( Mohamed A. Gomaa ),( Mohamed H. Refaat ),( Tamer M. Salim ),( Abo El-khair B. El-sayed ),( Makhlouf M. Bekhit ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 한국미생물·생명공학회지 Vol.47 No.3

        Chlorella vulgaris was isolated from the Nile River, Qalubia Governorate, Egypt, for possible use in biodiesel production. BG-II nutrient growth media was used for isolation and laboratory growth. Identification was performed via 18S rRNA gene amplification, followed by sequencing. The alga was exposed to UVC (254 nm) for 15, 30, and 45 s to improve dry weight accumulation and to increase the oil production. Daily measurements of dry weight (g·l<sup>-1</sup>) were performed; oil content and volumetric lipid productivity were also determined. UV-C exposure led to an increase in the volumetric lipid productivity by 27, 27.3, and 32.4 mg·l<sup>-1</sup>·d<sup>-1</sup> with 15, 30, and 45 s, respectively, as compared with the control, which resulted in 18 mg·l<sup>-1</sup>·d<sup>-1</sup>. Of the examined mutants, the one with the highest productivity was re-irradiated by UV-C (254 nm) for 15, 30, 45, and 60 s. For 15 s of exposure time, the oil content increased to 34%, while it was 31% at 30 s; further, it decreased to 22% at 45 and 60 s exposures. The fatty acid methyl ester profile was 82.22% in the first mutant at 45 s, compared with the wild strain that contained a total of 66.01% of FAs. Furthermore, the highest levels of polyunsaturated fatty acid methyl ester were observed in the mutant exposed for 45 s, and it reached 11.41%, which reduced the cetane number to 71.3.

      • SCOPUSKCI등재

        Identification of Green Alga Chlorella vulgaris Isolated from Freshwater and Improvement Biodiesel Productivity via UV Irradiation

        Gomaa, Mohamed A.,Refaat, Mohamed H.,Salim, Tamer M.,El-Sayed, Abo El-Khair B.,Bekhit, Makhlouf M. The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.3

        Chlorella vulgaris was isolated from the Nile River, Qalubia Governorate, Egypt, for possible use in biodiesel production. BG-II nutrient growth media was used for isolation and laboratory growth. Identification was performed via 18S rRNA gene amplification, followed by sequencing. The alga was exposed to UV-C (254 nm) for 15, 30, and 45 s to improve dry weight accumulation and to increase the oil production. Daily measurements of dry weight ($g{\cdot}l^{-1}$) were performed; oil content and volumetric lipid productivity were also determined. UV-C exposure led to an increase in the volumetric lipid productivity by 27, 27.3, and $32.4mg{\cdot}l^{-1}{\cdot}d^{-1}$ with 15, 30, and 45 s, respectively, as compared with the control, which resulted in $18mg{\cdot}l^{-1}{\cdot}d^{-1}$. Of the examined mutants, the one with the highest productivity was re-irradiated by UV-C (254 nm) for 15, 30, 45, and 60 s. For 15 s of exposure time, the oil content increased to 34%, while it was 31% at 30 s; further, it decreased to 22% at 45 and 60 s exposures. The fatty acid methyl ester profile was 82.22% in the first mutant at 45 s, compared with the wild strain that contained a total of 66.01% of FAs. Furthermore, the highest levels of polyunsaturated fatty acid methyl ester were observed in the mutant exposed for 45 s, and it reached 11.41%, which reduced the cetane number to 71.3.

      • Experimental shear strengthening of GFRC beams without stirrups using innovative techniques

        Marwa Hany,Mohamed H. Makhlouf,Gamal Ismail,Ahmed S. Debaiky 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.83 No.4

        Eighteen (18) (120×300×2200 mm) beams were prepared and tested to evaluate the shear strength of Glass Fiber Reinforced Concrete (GFRC) beams with no shear reinforcement, and evaluate the effectiveness of various innovative strengthening systems to increase the shear capacity of the GFRC beams. The test variables are the amount of discrete glass fiber (0.0, 0.6, and 1.2% by volume of concrete) and the type of longitudinal reinforcement bars (steel or GFRP), the strengthening systems (externally bonded (EB) sheet, side near-surface mounted (SNSM) bars, or the two together), strengthening material (GFRP or steel) links, different configurations of NSM GFRP bars (side bonded links, full wrapped stirrups, side C-shaped stirrups, and side bent bars), link spacing, link inclination angle, and the number of bent bars. The experimental results showed that adding the discrete glass fiber to the concrete by 0.6%, and 1.2% enhanced the shear strength by 18.5% and 28%, respectively in addition to enhancing the ductility. The results testified the efficiency of different strengthening systems, where it is enhanced the shear capacity by a ratio of 28.4% to 120%, and that is a significant improvement. Providing SNSM bent bars with strips as a new strengthening technique exhibited better shear performance in terms of crack propagation, and improved shear capacity and ductility compared to other strengthening techniques. Based on the experimental shear behavior, an analytical study, which allows the estimation of the shear capacity of the strengthened beams, was proposed, the results of the experimental and analytical study were comparable by a ratio of 0.91 to 1.15.

      • Shear strengthening of reinforced concrete beams using NSM/EBR techniques

        Abdel-kareem, Ahmed H.,Debaiky, Ahmed S.,Makhlouf, Mohamed H.,Abdel-baset, M. Techno-Press 2021 Advances in concrete construction Vol.12 No.1

        This paper presents the experimental results of research into the behavior of shear-enhanced reinforced concrete R.C beams using steel stirrups, Fiber Reinforced Polymers FRP rods, and Fiber Reinforced Polymers FRP strips. This enhancement was accomplished by the Near Surface Mounted technique NSM. The NSM technique contains a groove on the outside surface of the concrete member to adjust the depth to be less than the cover of the member. After cleaning, the epoxy paste was used to fill half of the groove's depth. In the groove, the particular FRP element is then installed. Finally, the groove is filled with epoxy and the outside surface of the concrete is levelled with so much epoxy. This method enables the fiber reinforcement polymer FRP materials is covered completely by epoxy. The objective of this research is to study the effect of NSM technique on shear resistance for stressed beam. 13 experimental studies of half-scale R.C beams were involved in this paper. The experimental program included two specimens strengthened with steel stirrups, eight specimens strengthened with stirrups of Glass Fiber Reinforced Polymer GFRP rods with the shape of deferent end anchorage and angle, two specimens strengthened with externally bonded GFRP strips. The remaining un-strengthened specimen was allocated for comparison as a control one. The test results included ultimate load of capacity, deflection, cracking, and failure mode. All beams enhanced with GFRP rods showed a capacity improvement ranging from 14% to 85% compared to the reference beam, and compared to the reference beam, beams enhanced with GFRP strips showed a capacity improvement ranging from 7% to 22%.

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