http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Studies on Improved Amylases Developed by Protoplast Fusion of Aspergillus species
( Tolulope Modupe Adeleye ),( Sharafadeen Olateju Kareem ),( Bankole Mobolaji Olufunmilayo ),( Olusegun Atanda ),( Michael Bamitale Osho ),( Olawale Dairo ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 한국미생물·생명공학회지 Vol.49 No.1
Improved amylases were developed from protoplast fusants of two amylase-producing Aspergillus species. Twenty regenerated fusants were screened for amylase production using Remazol Brilliant Blue agar. Crude enzyme extracts produced by solid state fermentation of rice bran were assayed for activity. Three variable factors (temperature, pH and enzyme type) were optimized to increase the amylase activity of the parents and selected fusants using rice bran medium and solid state fermentation. Analysis of this optimization was completed using the Central Composite Design (CCD) of the Response Surface Methodology (RSM). Amylase activity assays conducted at room temperature and 80℃ demonstrated that Aspergillus designates, T5 (920.21 U/ml, 966.67 U/ml), T13 (430 U/ml, 1011.11 U/ml) and T14 (500.63 U/ml, 1012.00 U/ml) all exhibited improved function making them the preferred fusants. Amylases produced from these fusants were observed to be active over the entire pH range evaluated in this study. Fusants T5 and T14 demonstrated optimal activity under acidic and alkaline conditions, respectively. Fusants T13 and T14 produced the most amylase at 72 h while parents TA, TC and fusant T5 produced the most amylase after 96 h of incubation. Response surface methodology examinations revealed that the enzyme from fusant T5 was the optimal enzyme demonstrating the highest activity (1055.17 U/ml) at pH 4 and a temperature of 40℃. This enzyme lost activity with further increases in temperature. Starch hydrolysis using fusant T5 gave the highest yield of glucose (1.6158 g/100 ml). The significant activities of the selected fusants at 28 ± 2℃ and 80℃ and the higher sugar yields from cassava starch hydrolysis over their parental strains indicate that it is possible to improve amylase activity using the protoplast fusion technique.
Methyl Jasmonate: Behavioral and Molecular Implications in Neurological Disorders
Oritoke Modupe Aluko,Joy Dubem Iroegbu,Omamuyovwi Meashack Ijomone,Solomon Umukoro 대한정신약물학회 2021 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.19 No.2
Methyl jasmonate (MJ) is a derivative of the jasmonate family which is found in most tropical regions of the world and present in many fruits and vegetables such as grapevines, tomato, rice, and sugarcane. MJ is a cyclopentanone phytohormone that plays a vital role in defense against stress and pathogens in plants. This has led to its isolation from plants for studies in animals. Many of these studies have been carried out to evaluate its therapeutic effects on behavioral and neurochemical functions. It has however been proposed to have beneficial potential over a wide range of neurological disorders. Hence, this review aims to provide an overview of the neuroprotective properties of MJ and its probable mechanisms of ameliorating neurological disorders. The information used for this review was sourced from research articles and scientific databases using ‘methyl jasmonate’, ‘behavior’, ‘neuroprotection’, ‘neurodegenerative dis-eases’, and ‘mechanisms’ as search words. The review highlights its influences on behavioral patterns of anxiety, ag-gression, depression, memory, psychotic, and stress. The molecular mechanisms such as modulation of the antioxidant defense, inflammatory biomarkers, neurotransmitter regulation, and neuronal regeneration, underlying its actions in man-aging neurodegenerative diseases like Alzheimer’s and Parkinson’s diseases are also discussed. This review, therefore, provides a detailed evaluation of methyl jasmonate as a potential neuroprotective compound with the ability to modify behavioral and molecular biomarkers underlying neurological disorders. Hence, MJ could be modeled as a guided treat-ment for the management of brain diseases.
Joan Modupe Babajide,Solomon Maina,Benjamin Kiawa,Robert Skilton 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.3
Gbodo is a traditional Nigerian fermented dried yam food using tubers or slices. Identification of microbes using conventional methods reveals only a fraction of the true microbial community. Forty-five fungal isolates associated with gbodo at 0, 6, 12, 18, and 24 h of steeping/ fermentation were identified using molecular techniques. Ribosomal RNA-ITS fragments of genomic DNA were amplified using ITS1 and ITS4 primers and subjected to nuclecotide sequence determination. Meyerozyma gulliermondii represented 56-78% of isolates during 24 h of yam steeping. Sixty-seven percent of all identified isolates were members of the genera Meyerozyma and Pichia. The biodiversity index of fungal isolates increased from 0.33 at 0 h to 0.56 at 12 h. M. guilliermondii exhibited a resilient presence in isolates.
Primary oral soft tissue angiosarcoma of the cheek: a case report and literature review
Benjamin Fomete,Modupe Samaila,Sunday Edaigbini,Rowlan Agbara,Uche Albert Okeke 대한구강악안면외과학회 2015 대한구강악안면외과학회지 Vol.41 No.5
Angiosarcoma is a rare and aggressive malignant tumor that has a poor prognosis. It represents less than 1% of all malignancies occurring in the oral cavity and salivary glands. We present a 35-year-old male with angiosarcoma of the cheek following traumatic injury and a review of the current litera- ture.
Child Abuse and the Eye in an African Population
Michaeline A Isawumi,Modupe Adebayo 대한안과학회 2017 Korean Journal of Ophthalmology Vol.31 No.2
Purpose: There is a paucity of data on child abuse and the eye in West Africa. Therefore, a need exists to determinethe causes and circumstances surrounding these occurrences and their effects on the eye and visionin a pediatric population. Methods: A 3-year study of consecutive cases of ocular features that presented to an eye clinic following ahistory suggestive of child abuse along with any associated eye injury was carried out. Socio-demographiccharacteristics were obtained, as were the presenting and posttreatment visual acuity, and a full clinical eyeexamination was also done. Results: Fifty-six children with 56 affected eyes were examined. The male to female ratio was 1.8 : 1; the meanage was 8.25 ± 3.5 years; and the subjects were predominately primary school children 30 (53.6%). The mostcommon ocular disorders were conjunctiva hyperemia (46.4%), corneal abrasion/ulcer (25.4%), hyphema/uveitis (42.9%), retinal detachment (7.1%), sclera rupture (7.1%), and globe perforation (21.4%). The intraocularpressure was raised in 17.9% of participants. Presenting versus posttreatment visual acuities showed normalvision in four (7.1%) vs. eighteen (32.1%), visual impairment in 12 (21.4%) vs. six (10.7%), severe visual impairmentin 20 (35.7%) vs. 12 (21.4%), and blindness in 12 (21.4%) vs. 14 (25%), p < 0.001. The most commoninjurious agents were canes (25.0%) and sticks (17.9%). The people who inflicted the injuries were parents/relatives (uncles) in 35.7% of cases and the children themselves (accidents) in 21.4% of cases. Circumstancespredisposing participants to eye injuries were child labor in 39.3% and beatings in 25.0%. Complications werecataracts (25.0%), corneal opacity (39.3%), and retinal detachment (7.1%). Conclusions: Injuries commonly resulted from canes and sticks used during beatings, as well as from childlabor, corporal punishment, accidents, and assaults. Legislation and laws need to be enacted to prevent negativepsychosocial and economic impacts on the child, family and country as a whole.