Flow cytometric immunophenotyping of canine adipose-derived mesenchymal stem cells (ADMSCs) and feline ADMSCs using anti-human antibodies

Ko, Minho,Lee, Kwon Young,Kim, Sae Hoon,Kim, Manho,Choi, Jung Hoon,Im, Wooseok,Chung, Jin Young The Korean Society of Veterinary Science 2018 大韓獸醫學會誌 Vol.58 No.1

Various trials have been conducted to develop therapies for serious untreatable diseases. Among these, those using stem cells have shown great promise, and adipose-derived mesenchymal stem cells (ADMSCs) are easier to obtain than other types of stem cells. Prior to clinical trials, characterization of ADMSCs with monoclonal antibodies should be performed. However, it is difficult to use species-specific antibodies for veterinarians. This study was conducted to confirm the panel of human antibodies applicable for use in immunophenotypic characterization of canine adipose-derived stem cells and feline ADMSCs extracted from subcutaneous adipose tissue collected during ovariohysterectomy. For flow cytometric immunophenotyping, the third passages of canine ADMSC and feline ADMSC and human CD31, CD34, CD42, CD44, CD62 and CD133 antibodies were used. Of these, CD133 reacted with canine cells (3.74%) and feline cells (1.34%). CD133 is known as a marker related with more primitive stem cell phenotype than other CD series. Because this human CD133 was not a species-specific antibody, accurate percentages of immunoreactivity were not confirmed. Nevertheless, the results of this study confirmed human CD133 as a meaningful marker in canine and feline ADMSCs.

Control level simulation of an automatic storage and retrieval system in the automotive industry

Ko, Minsuk,Park, Sang C,Chang, Minho SAGE Publications 2013 Concurrent engineering Vol.21 No.1

<P>Conventional simulators have focused on the abstract aspects of an automatic storage and retrieval system, which mainly deals with design verification, alternative comparison, and system diagnosis. Although such simulators can provide overall system visibility by monitoring how well the process works, the simulation models are not sufficiently realistic for detailed design and implementation purposes. To address this problem, we propose a method of control level simulation of an automatic storage and retrieval system in an automobile plant. The proposed method involves four major steps: (1) designing the process and layout for effective storage and retrieval; (2) abstract simulation of the automatic storage and retrieval system; (3) preparing the mechanical design to obtain three-dimensional kinematic models and the electrical design to produce a control program and a plant model; and (4) control level simulation of the automatic storage and retrieval system, including both manual mode and automode simulations via a human–machine interface. The major benefit of the proposed method is the reduction of the construction time and effort required to validate the programmable logic controller program of a real automatic storage and retrieval system, since potential errors can be detected and fixed before actual implementation.</P>

An Effective XML Schema Conversion Technique for Improving XML Document Reusability using Pattern List

Ko, Hye-Kyeong,Yang, Minho The Institute of Internet 2017 International Journal of Internet, Broadcasting an Vol.9 No.2

The growing use of XML markup language has made amount of heterogeneous. XML documents are widely available in the Web. As the number of applications that utilize heterogeneous XML documents grow, the importance of XML document extraction increases greatly. In this paper, we propose a XML schema conversion technique that converts reusable XML schema from XML documents. We convert the schema graph and we use the reusability pattern list. The converted XML schema is evaluated in terms of cohesion, coupling, and reusability. The converted XML schema could be used to construct databases for various fields where XML is used as an intermediation of data exchange.

Free-standing molybdenum disulfide/graphene composite paper as a binder- and carbon-free anode for lithium-ion batteries

Yang, MinHo,Ko, Seunghyun,Im, Ji Sun,Choi, Bong Gill Elsevier 2015 Journal of Power Sources Vol.288 No.-

<P><B>Abstract</B></P> <P>Two dimensional nanosheets, such as graphene and metal disulfides, have attracted a great deal of attention as anode materials for lithium-ion batteries, owing to their unique capability for lithium-ion storage. In this work, we integrate graphene and MoS<SUB>2</SUB> nanosheets into a free standing film form using a simple vacuum filtration method. As-prepared composite film could be readily employed as a binder- and carbon-free anode for lithium-ion batteries, removing the polymeric binders and conductive carbon additives that are required for the preparation of conventional electrodes. In addition, the interconnected structure of graphene and MoS<SUB>2</SUB> sheets provide a good electrical conductivity to the entire film electrode. When tested electrochemical performance as an anode for lithium-ion batteries, the composite film electrode exhibits superior performance compared to the exfoliated MoS<SUB>2</SUB> electrode, such as 65.8% capacity retention at a high current rate of 1000 mA g<SUP>−1</SUP> and 91.1% capacity retention after 100 cycles.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A free-standing MoS<SUB>2</SUB>/graphene (MoS<SUB>2</SUB>/G) composite paper was prepared by a simple vacuum filtration method. </LI> <LI> HADDF-STEM image revealed an alternatively layered structure of MoS<SUB>2</SUB>/G composite paper. </LI> <LI> MoS<SUB>2</SUB>/G paper significantly improved the Li<SUP>+</SUP> ion storage/release process, compared to the exfoliated MoS<SUB>2</SUB>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Metal alloy hybrid nanoparticles with enhanced catalytic activities in fuel cell applications

Kim, Minho,Lee, Chungyeon,Ko, Sung Min,Nam, Jwa-Min Elsevier 2019 Journal of solid state chemistry Vol.270 No.-

<P><B>Abstract</B></P> <P>Due to the depletion of fossil fuels and severe environmental pollution problems, the urgent demand for clean and sustainable energy sources is constantly emphasized. In recent decades, platinum (Pt)-based fuel cells have achieved tremendous advances as a green energy source, but there is still a need to further improve energy production efficiency in order to realize practical applications. Conventional Pt electrocatalysts are usually insufficient in catalytic activity and the selectivity of electrochemical reactions due to sluggish kinetics and surface poisoning. In contrast, metal alloy nanocatalysts exhibit exceptional electrocatalytic performance on fuel cells, more than pure Pt catalysts, owing to the synergistic effect by systematic modification of the surface geometry and electronic structure of the catalytically active metal component. This review provides overviews and advances in the applications of metal alloy nanohybrid particles enabling the improvement of electrocatalytic efficiency and discusses the challenges and outlooks in the fields of green energy production based on metal alloy nanocatalysts.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Metal alloy nanocatalysts exhibit exceptional electrocatalytic activity on fuel cell. </LI> <LI> Advances of metal alloy particles enhancing electrocatalytic activity are reviewed. </LI> <LI> Outlooks in the field of fuel cell based on metal alloy nanocatalysts are discussed. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Synergistic effects (<I>d</I>-band shift, surface-lattice strain and surface-defects formation) of metal alloy nanocatalysts for the improvement of electrochemical activity.</P> <P>[DISPLAY OMISSION]</P>

Extraction of Saccharina japonica Ingredients Using Pulsed Electric Fields for Value Generation from Marine Biomass

Byeongwoo Ko,Seunghye Kang,Minho Kim,Mangi Cho,Antonio Delgado,Alexander Jahn 한국산업식품공학회 2020 학술대회 및 심포지엄 Vol.2020 No.11

Currently, domestic seaweed production is about 800,000 tons per year, which is the largest seaweed production in the world after China and Japan. However, most seaweeds (Pyropia ssp, Undaria pinnatifida, Saccharina japonica, etc) are consumed in the form of dried products or hot water extracts, and there are many difficulties in developing various products due to difficulties in the manufacturing process. Through the pulsed electric field treatment device, the extraction efficiency can be expected to increase compared to the convectional hot water extraction. Pulsed electric field technology is a non-heating treatment technology in which high-voltage electric energy is processed into cells in the form of pulses, affecting the potential difference of cell membranes, and performing collapse or perforation treatment. In this study, components such as protein and carbohydrate content were extracted by treating Saccharina japonica with PEF, and compared with the hot water extraction method, As a result of experiment at voltages of 0.7 and 1.5㎸ and pulse duration of 5㎲, the crude protein content increased by up to 42% and the sugar content increased by up to 30% compare to the hot water extraction. Higher efficiency results can be expected through optimization of several parameters that may affect the extraction process.

Dealloyed Intra-Nanogap Particles with Highly Robust, Quantifiable Surface-Enhanced Raman Scattering Signals for Biosensing and Bioimaging Applications

Kim, Minho,Ko, Sung Min,Kim, Jae-Myoung,Son, Jiwoong,Lee, Chungyeon,Rhim, Won-Kyu,Nam, Jwa-Min American Chemical Society 2018 ACS central science Vol.4 No.2

<▼1><P/><P>Uniformly controlling a large number of metal nanostructures with a plasmonically enhanced signal to generate quantitative optical signals and the widespread use of these structures for surface-enhanced Raman scattering (SERS)-based biosensing and bioimaging applications are of paramount importance but are extremely challenging. Here, we report a highly controllable, facile selective-interdiffusive dealloying chemistry for synthesizing the dealloyed intra-nanogap particles (DIPs) with a ∼2 nm intragap in a high yield (∼95%) without the need for an interlayer. The SERS signals from DIPs are highly quantitative and polarization-independent with polarized laser sources. Remarkably, all the analyzed particles displayed the SERS enhancement factors (EFs) of ≥1.1 × 10<SUP>8</SUP> with a very narrow distribution of EFs. Finally, we show that DIPs can be used as ultrasensitive SERS-based DNA detection probes for detecting 10 aM to 1 pM target concentrations and highly robust, quantitative real-time cell imaging probes for long-term imaging with low laser power and short exposure time.</P></▼1><▼2><P>We developed a synthetic strategy for forming dealloyed intra-nanogap particles in a high yield, and these nanoparticles with strong, stable, and quantifiable Raman signals were used for ultrasensitive DNA detection and target-specific cell imaging.</P></▼2>

An Effective XML Schema Conversion Technique for Improving XML Document Reusability using Pattern List

Hye-Kyeong Ko,Minho Yang 한국인터넷방송통신학회 2017 International Journal of Internet, Broadcasting an Vol.9 No.2

The growing use of XML markup language has made amount of heterogeneous. XML documents are widely available in the Web. As the number of applications that utilize heterogeneous XML documents grow, the importance of XML document extraction increases greatly. In this paper, we propose a XML schema conversion technique that converts reusable XML schema from XML documents. We convert the schema graph and we use the reusability pattern list. The converted XML schema is evaluated in terms of cohesion, coupling, and reusability. The converted XML schema could be used to construct databases for various fields where XML is used as an intermediation of data exchange.

Bio-inspired frictional pads made of CNT composites for high temperature process

Hoon Yi,Minho Sung,Insol Hwang,Hangil Ko,Hyun-ha Park,Hoon Eui Jeong 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.11

During the assembly process of semiconductors or display devices, silicon wafers or glass substrates, which are usually very thin, large, and fragile, should be carefully handled with high accuracy. Moreover, there should be no surface contamination on the substrates. Thus, bio-inspired mushroom-shaped adhesive pads have strong potential to replace existing techniques such as vacuum suction or electrostatic chucks since dry adhesives enable precise manipulation of various substrates without any surface contamination in a reversible, repeatable, and durable manner. To fabricate mushroom-shaped dry adhesives, poly(dimethyl siloxane) (PDMS) have been widely used because they have favorable mechanical properties such as low elastic modulus and high elongation break, which allows for simple and reliable prototyping. Furthermore, the low elastic modulus of the PDMS allows the replicated structures to make a conformal contact against varying substrates even under low preload and therefore exhibit a high level of adhesion strength. In spite of these advantages, the PDMS shows inherent limitations in maintaining its form and adhesion strength at high temperature (> 200 ℃), which brings restrictions on practical application for various high-temperature manufacturing process. To address this limitation, we suggest bio-inspired adhesive pads using carbon nanotube (CNT) composite which have enhanced durability and stability at high-temperature conditions.

Systems Metabolic Engineered of Escherichia coli Using Synthetic Regulatory Small RNA for the Production of Putrescine and L-Proline

Yoo-Sung KO,Minho ROH,Seung Min YOO,Sang Yup LEE 한국생물공학회 2019 한국생물공학회 학술대회 Vol.2019 No.4