乳房疾患의 臨床的 硏究

孫基燮,姜珉圭 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

The clinical study on 119 cases of breast diseases was carried out in the department of general surgery, college hospital of Chungnam National University for last 5 years. The results are as follows: 1) The most common lesion was breast cancer, in 47 cases, 39.5% and followed by fibrocystic disease in 36 cases, 30.3%, fibroadenoma in 19 cases, 15.9% in order. 2) The peak age incidence of breast cancer is fifth and sixth decades, 16 cases, 34% each, fibrocystic disease in third, fourth, and fifth decades, 30% each and fibroadenoma is third decade, 11 cases, 60%. 3) The most common chief complaint was palpable mass in 94%. The size of masses of cancer were larger than those of benign lesions. 4) The location of lesions, both cancer and benign diseases were more prominant in the right side and the rate is 5 : 4. 5) Both breast cancer and benign diseases were most commonly found in the upper outer quadrant and followed in the central portion. 6) The accuracy of preoperative diagnosis of breast cancer is 93.6%, fibroadenoma is 100%, and fibrocystic diseases is 72.2% respectively. 7) The most frequent region of metastasis was bone in 6 cases, 12.8% and followed by lung and pleura, and skin in 3 cases, 6. 4% respectively. 8) The surgical procedures were performed with modified radical mastectomy, which is the most common, in 34 cases, 72.4%, radical mastectomy in 10 cases, 21.3% simple mastectomy in 3 cases, 6.3% respectively for breast cancer. The excisional biopsy were performed for almost all cases of benign lesions in 64 cases, 95.5%.

대장 및 직장암의 임상적 연구

孫基燮,姜珉圭 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

The colorectal cancer is the second most common cause of cancer deaths in the United States. The incidence of colorectal cancer is seemed to increase lately in our country as the dietary condition has been westernized. In addition that various diet, life style, hormonal, psychological variables and other unknown environmental factors may affect colorectal cancer. But earlier detection with numerous favorable means and appropriate surgical treatment for colorectal cancer used to improve survival and the quality of life of patients after surgical therapy. In this reguard authors reviewed 105 cases of colorectal cancers had been treated at the Department of General Surgery, College of Medicine, Chungnam National University from July 1984 to June 1988. The results are as follows; 1) The peak incidence reveales in the sixth decade and followed by seventh decade and those are 64.7%. The sex ratio is predominant in male as 1.1:1 2) The location of lesion is most frequent in the rectum(61.0%) and followed by sigmoid colon (16.2%). 3) The common symptoms and signs are bloody stool and melena, pencil like stool, constipation, abdominal pain and defication difficulty. 4) Major diagnostic methods and those accuracy are barium enema 96.2% sigmoidoscopy 92. 1 %, rectal. examination 54.3% and flexible fiberoptic colonoscopy 85.7%. 5) Pathologic diagnosis of patients were mostly adenocarcinoma 94.8%, which were moderately differentiated 66.7% and well differentiated 18.5%. 6) Dukes' classification shows. A: 16.7%, B: 19.8%, C:29.2%, D:34.4% and pathological staging shows. 1 :16.7%, Ⅱ:19.8%, Ⅲ:32.3%, Ⅳ:31.3%. 7) No correlation was found between the level of CEA and pathological grade and stage. 8) Major operative procedure were as follows; Miles' operation: 29.0%, sigmoid loop colostomy: 21.%, right hemicolectomy: 15.0%, anterior resection: 14.0%, lower anterior resection:9.0%. 9) Overall postoperative complication was 38.0% which include wound infection 34.7%, bladder dysfunction 24.5%, intestinal obstruction 18.4%, and leakage 10.2% and operative mortality is none. 10) Distant metastasis developed in the liver, 19.0% and in pelvic organ, 12.4%. 11) 4 years follow up shows as follows, 1 year survival rate is 83.0%, 2 years 61.5%, 3 years 30.4% and 4 years 29.6%.

Polydyn^�(Tissue Extract)의 각종 암환자에 대한 투여 효과

손기섭,강민규 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

Polydyn(tissue extract) was administrated intramusculary, 2 ampules (120mg) a day, on 21 cancer patients and effects on pain, would healing, granulation tissue proliferation, ascites and side effects were analyzed. The results were as follows: 1. Improvement of pain was noted on 83.3% after Polydyn administration and 83.3% belonged to Visick score I or II. 2. On wound healing, 6 patients (100%) were healed clearly. 3. 50% of Polydyn injection group showed prominent proliferation of granulation tissue on wound than non-injection group. 4. Effect on ascites was unclear. 5. No side effect was noted during Polydyn administration.

부분 무치증 환아의 증례보고

손정민,최남기,김선미,양규호 大韓小兒齒科學會 2007 大韓小兒齒科學會誌 Vol.34 No.4

부분 무치증은 제 3대구치를 제외한 6개 이상의 선천적 치아 결손으로 정의된다. 제3대구치를 제외한 선천적 치아 결손의 유병율은 1.6∼9.6%이며 부분 무치증의 유병율은 O.08∼1.1%이다. 가장 많이 이환되는 치아는 하악 제 2소구치이며 그 다음은 상악 측절치,상악 제2소구치 순으로 호발하며 영구치에 비해 유치에서는 드물고 여성에게 호발하는 경향이 있다. 부분 무치증은 외배엽 이형성증과 같은 특정 증후군과 연관되거나 치판의 생리적인 장애나 파열,공간적 제한,치성 상피의 기능적인 비정상,기저 간엽세포의 유도 실패나 유전적 영향 등의 원인에서 유래된다고 알려져 있다. 부분 무치증은 안모의 심미적 문제와 함께 치아의 교합 이상 등 기능적인 장애가 야기될 수 있으므로 임상 검사와 방사선 검사를 통한 조기 진단이 필요하며,이에 따른 적절한 치료계획이 요구된다. 이에 본 증례에서는 특별한 전신질환이 없는 부분 무치증 환아의 구강내 소견 및치료경과에 대해 보고하고자 한다. Oligodontia is defined as the congenital absence of six or more teeth in dentition, excluding the third molars. The prevalence of congenital missing teeth is about 1.6 to 9.6% of population and the prevalence of oligidontia is about 0.08 to 1.1%. The mandibular second premolar is the most frequently absent after the third molar, followed by the maxillary lateral incisor and upper second premolar. Females seem to be affected slightly more than males. Oligodontia may occur either in isolation, or as a part of a syndrome such as ectodermal dysplasia. Different causes are possible for oligodontia: physical obstruction or distruction of the dental lamina, space limitation, functional abnormalities of the dental epithelium, failure of induction of the underlying mesenchyme, chemotherapy, radiotherapy or genetic factor. Because oligodontia would result in esthetic and functional problems, such as facial asymmetry or occlusal disharmony, early diagnosis from clinical and radiographic examination was necessary. And appropriate treatment plan should be followed. This case report was about oral conditions and treatment of the oligodontia patients who have no specific systemic disease.

상아질 접착제의 미세누출에 관한 연구

손정민,최남기,김선미,양규호,박지일 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.4

본 연구는 여러가지 상아질 접착제들의 법랑질 변연과 상아질 변연에서의 미세누출을 비교 평가하기 위해 이루어졌다. 발거된 건전한 영구치의 협면과 설명에 5급 와동을 형성하고 형성된 50개의 와동을 무작위로 5개의 군으로 나누어 각군에 Adper™ Scotchbond™ Multi-purpose Plus Adhesive (SM; 3M ESPE. USA), Adper™ Single bond 2 (SB; 3M ESPE, USA), Clearfil™ SE Bond (SE; Kuraray Medical Inc., Japan), Adper™ Prompt™ L-Pop™ (PL; 3M ESPE, USA), G-Bond™ (GB; GC Co., Japan)를 적용하고 Filtec™ Z350 A3 (3M ESPE. USA)로 충전하였다. 시편을 37℃ 증류수에 24시간 보관한 후 5℃와 55℃에서 10초씩 1000회 열순환하고 2% methylene blue 용액을 24시간 넣어 염색한 뒤, 주수 하에 치아를 협설로 절단하여 색소의 침투 정도를 화상분석현미경을 이용하여 침투깊이를 측정한 뒤 각 군간의 미세누출 정도를 비교하여 침투깊이에 따라 0~3점으로 분류하여 다음과 같은 결과를 얻었다. 1. 범랑질 변연에서의 미세누출은 PL(0.85), SB(0.55), GB(0.50), SM(0.35), SE(0.25) 순이었으며 PL은 SM 및 SE와 통계학적으로 유의한 차이를 나타냈다(p<0.05). 2. 상아질 변연에서의 미세누출은 GB(2.10), SE(1.45), PL(1.40), SB(1.05), SM(0.70) 순이었으며 GB는 SB 및 SM과 통계학적으로 유의한 차이를 나타냈다(p<0.05). 3. 각 실험재료 군내에서 법랑질 변연에 비해 상아질 변연에서 미세누출이 더 크게 나타났으며, SE, PL, GB에서 통계학적으로 유의한 차이를 나타냈다(p<0.05). The objective of this study was to compare the microleakage of five adhesive systems in the enamel and dentin of permanent teeth. Class V cavity preparations with occlusal margins in enamel and gingival margins in dentin were prepared on both buccal and lingual surfaces of 25 extracted human molar teeth. The tested adhesives were: Adper Scotchbond Multi-purpose Plus Adhesive (SM), Adper Single bond 2 (SB), Clearfil SE Bond (SE), Adper Prompt L-Pop (pL) and G-Bond (GB). The results were as follows: 1. At the enamel margins, PL showed the highest leakage value(0.85), and others showed values of SB(0.55), GB(O.50), SM(O.35) and SE(0.25) in decreasing order. There were statistically significant differences in PL vs. SM and PL vs. SE(p<O.05). 2. At the dentin margins, GB showed the highest leakage value(2.10), and others showed values of SE(1.45), PL(1.40), SB(1.05), SM(0.70) in decreasing order. There were statistically significant differences in GB vs. SB and GB vs. SM(p<O.05). 3. Dentin margins showed high dye penetration rate than enamel margins in all material tested groups and there were statistically significant differences for SE, PL and GB.

Diluents and Cryoprotectants for Cryopreservation of Filefish Thamnaconus modestus Sperm

Le, Minh-Hoang,Lim, Han-Kyu,Min, Byung-Hwa,Son, Maeng-Hyun,Lee, Jung-Uie,Chang, Young-Jin The Korean Society of Fisheries and Aquatic Scienc 2008 韓國養殖學會誌 Vol.21 No.1

The present study aimed to find the best diluent and cryoprotectant for sperm cryopreservation of filefish Thamnaconus modestus. Two kinds of artificial seminal plasma(ASP1, ASP2), 0.3 M glucose and marine fish Ringer's solution(MFRS) were employed as diluent. Dimethyl sulfoxide(DMSO) and methanol as cryoprotectant were selected for sperm cryopreservation. Sperm was diluted at the ratio of 1:3 with diluents containing cryoprotectants and adjusted for final concentration at 5%, 10%, 15% and 20%. Mixed milt was frozen at liquid nitrogen vapor after equilibration for 5 min. The highest motility($40.5{\pm}2.8%$) and swimming speed($81.5{\pm}4.1{\mu}m/s$) of frozen/thawed sperm were observed in ASP1 diluent containing 10% DMSO and in ASP2 containing 15% DMSO, respectively. Results showed that cryopreservation with ASP as diluent and DMSO as cryoprotectant could be adopted for long term storage of filefish sperm.

지속성 외래 복막투석 환자의 복막염

신영태,황평주,김종학,강민규,구영선,양종오,장윤경,이강욱 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

CAPD has established itself as an effective method maintaining the patients with end stage renal disease. CAPD peritonitis is one of the most important complication of peritoneal dialysis. We analyzed the incidence, pathogens, and outcome of the peritonitis, of the 95 patients who underwent CAPD at CNUH from January 1993 to July 1998. The result were as follows : 1) A total of 72 episodes of peritonitis occurred during this periods. The incidence of peritonitis were 0.64/patient/year. 2) The first episode of peritonitis was occurred within a year in 75%, within two year in 95.8%. 3) The rate of positive and negative culture were 27.8% and 72.2% respectively. Gram positive organisms were cultured in 60%, Gram negative organisms in 40%. S. aureus were most prevalent organisms(30%). 4) The cure rate were 83.3% in Gram positive organisms and 62.5% in negative organisms. 5) Peritoneal catheter were removed in 18 cases. Among them, 7 cases are due to peritonitis. In conclusion, improved technology and increasing patients' effort to careful management of catheter will reduce the incidence of peritonitis.

신이식에서 이식신의 생존분석

오선미,김종학,황평주,구영선,강민규,나기량,김종섭,김성숙,이강욱,신영태,설종구,배진선,손기섭 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

To investigate the prognostic factors for the survival of transplanted kidney in patients with end-stage renal failure, 59 cases of renal transplantation from September 1986 to Feburary 1997 in Chungnam National University Hospital were analyzed retrospectively. The results were as follows: 1)The mean age of recipients was 33.8 years and that of doners was 38.9 years. The male to female ratio of recipients was 2.5:1, and that of donors was 1.03:1 2) Living related donore(LRD) were 79.6% and living non-related donors were 20.3%. The HLA-identical donors(ID) in LRD were 18.7% and HLA-haploidentical donors(HID) were 61.0%. In living non-related donors(LNRD), mean matched HLA-AB antigens were 1.56 and mean matched HLA-DR antigens were 0.56. 3) The average 5-year patient survival fate was 94%, and average 5-year graft survival rate was 70%. The 5-year graft survival fate of HLA-ID was 100%, and those HLA-HID and LNR were 70% and 36% respectively. 4) Total 33 episodes of acute rejection were found in 45.8% of transplanted patients. The number of acute rejection episode did not show significant difference between LRD and LNRD(P > 0.05). There was no significant difference in MLC between two groups(p > 0.05). 5) In comparison between the 18 patients who lost their graft function in 5 years and 17 patients who are maintaining graft function for more than 5 years, MLC was significantly lower in patients with functioning graft than of patients with non-functioning graft (p < 0.05). The number of rejection episode was also lower in patients with functioning graft than that of the patients with non-functioning graft(p < 0.05). However, there were no significant difference in recepient and donor age and history of pre-transplantation donor specific transfusion between two groups. With the results above, we can speculate that adequate donor selection according to good matched HLA typing and low MLC is very important for graft survival in renal transplantation. Prevention and treatment of acute rejection in renal transplantation is also na important factor for graft survival.

전신성 홍반성 낭창의 임상적 고찰

신영태,김종학,장윤경,양종오,구영선,강민규,황평주,나기량,이강욱,서광선 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

The epidemiology, diagnostic criteria, clinical features, symptoms and signs laboratory findings, kidney pathology, and clinicopathologic correlation of systemic lupus erythematosus(SLE) were analyzed. The 63 patients studied were managed at the Department of Internal medicine, CNUH, from January 1983 to December 1997. Kidney biopsy was performed in 53 patients out of 63 patients with SLE. The results were as follows: 1. The ratio of male to female was 1:26. They were 12 to 71 years old and mean age was 32.1 years old. The peak age incidence was 4th decades(30%). 2. The most frequent chief complaint on admission was generalized edema. Most patients complain two or more symptoms. 3. Immunologic and renal disorders were the most frequently observed in the ARA criteria of SLE. And the positive ANA, hematologic disorder, malar rash, and arthritis, were observed in order of frequency. 4. Among the 53 patients with renal biopsy, 30 patients revealed class Ⅳ lupus nephritis(56%), class II in 12 patients(23%), class V in 8 patients(15%) and class III in 2 patients(6%). 5. The cases of lupus nephritis represented as nephrotic syndrome were high in the class IV with 68% and class V with 86%. 6. Of 30 patients who can be followed up, 5 patients resulted in death(17 % of mortality). They "were 14 to 57 years old and mean age was 28 years old. Follow up duration were from 1 month to 6 years and 3 months, and mean duration was 1 year and 1 month, but 3 cases 7. The causes of death were as follows: Two cases were due to seizure and coma resulted from CNS involvement. One was sepsis due to miliary the & pneumonia. One was dilated cardiomyopathy and heart failure due to cardiac ac involvement. And the other one was sepsis and acute renal failure resulted from cellulitis. 8. Prednisolone was used in all patients basically, and methyl-prednisolone pulse therapy, antimalarials and alkylating agents were used in some cases. In the cases of combined therapy, the activity of SLE was well controlled.

Effects of Chungkookjang Extract on Growth Hormone Secretion from GH3 Mouse Pituitary Cell and Growth Hormone Receptor Signaling Pathway

Sun Il Choi(최선일),Ji Eun Kim(김지은),In Sik Hwang(황인식),Hye Ryun Lee(이혜련),Young Ju Lee(이영주),Hong Joo Son(손홍주),Dong Seob Kim(김동섭),Kyu Min Park(박규민),Dae Youn Hwang(황대연) 한국생명과학회 2012 생명과학회지 Vol.22 No.9

뇌하수체 전엽에서 성장호르몬의 생산과 분비는 세포의 분열과 분화 그리고 이동을 조절하는 몇 가지 천연물질에 의해 유도된다. 따라서 발효과정을 통해 제조된 청국장이 성장호르몬의 대사에 미치는 영향을 평가하기 위하여 성장호르몬 분비능과 반응성을 뇌하수체 세포와 성장호르몬 표적세포에서 관찰하였다. 6가지 종류의 콩 품종으로 제조된 청국장 추출물 중에서, 대원, 대풍, 태광의 3종류 청국장 추출물은 5 mg/ml 농도에서 GH3 세포로부터 성장호르몬의 분비를 촉진하였다. 비록 세포 생존능은 이러한 추출물에 의해 유의적인 변화가 유도되지 않았으나, 성장호르몬의 분비량은 청국장 추출물의 농도에 의존적으로 증가하였다. 또한 성장호르몬의 표적 기관으로부터 유래된 MG63과 HepG2 세포는 GH3로부터 수집된 조건적 배양액에 의해 유의적으로 활성화되었다. 또한 이러한 세포에서 STAT5 발현은 대원 청국장 추출물을 처리 후, 15분 혹은 30분부터 세포질에서 유의적으로 증가하였으며, p-STAT5는 핵에서 30분 혹은 60분부터 증가하였다. 따라서 이러한 결과는 3가지 종류의 청국장 추출물은 성장호르몬의 분비를 촉진시키며, 청국장의 조건적 배양액은 성장호르몬 표적세포에서 신호전달을 유도함을 제시하고 있다. The production and secretion of growth hormone (GH) in the anterior pituitary gland can be induced by several natural products to control cell proliferation, differentiation, and migration. To investigate whether Chungkookjang (CKJ) produced by the fermentation process affects GH-related metabolism, the secretion and the response of GH were observed in pituitary cells and GH target cells. Among six CKJs manufactured by different strains of glycine max, only three CKJs, including Daewon (DW), Daepung (DP), and Taegwang (TG), induced GH secretion from GH3 cells at 5.0 mg/ml concentration. There were no significant changes detected in the viability of any of the cells treated with these CKJs. In addition, the increase in GH secretion from the GH3 cells was dependent on the concentration of the three types of CKJs. The proliferation of cell lines, including MG63 and HepG2 cells, that originated from those derived from the GH target organs was significantly activated by treatment with the GH-containing conditional medium (GCM) harvested from the three CKJ-treated GH3 cells, although their induction rate was different from each other. In these cells, p-STAT5 was maximally translocated into the nucleus of MG63 cells 30 min after DW treatment, while it was translocated in HepG2 cells at 60 min. These results suggest that these three types of CKJ could enhance the secretion of GH, as well as the GCM-derived response, in the two target organs.