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RISS 인기검색어
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- 저자 : Miao Xuexia (검색결과 2 건)
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Studies on the Hereditary Properties of SSR Marker in Silkworm (Bombyx mori L.)
Li Muwang,Li Minghui,Miao Xuexia,Lu Cheng,Huang Yongping Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.11 No.1
Two BC1 group, c108 $\times$ (p50 $\times$ c108) and p50 $\times$ (p50 $\times$ c108), one group of F$_{2}$ progeny, (p50 $\times$ c108) F$_{2}$ ,and 3 SSR markers, F10539, FlO626 and FlO618 were used to test the hereditary properties of SSR markers in silkworm. FI0539, FlO626 were proved to be linkage, and FlO618 was proved to be independent to those two markers. According to Mendel's law, the recombinant value between F10539, FlO626 was calculated in all of these groups, and they were 8.55$\%$ (c108BC1), 8.02$\%$ (p50BC1) and 7.81 $\%$ (F$_{2}$) respectively. There was dominant difference among the crossing-over value using paired-samples tests by SPSS 10.0 software. This research proved that SSR markers were co-dominant in B. mori too, and F 2 progeny could be used to construct SSR linkage map although B. mori lacked of crossing over in females.
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Identification and phylogeny of five male-specific lethal genes in the silkworm Bombyx mori
Wenbin LIU,Yong ZHANG,Xuexia MIAO,Yongping Huang 한국곤충학회 2008 Entomological Research Vol.38 No.-
In the fruit fly (Drosophila melanogaster) dosage compensation equalization of X-linked genes between the sexes is achieved via a complex that is termed the compensasome, which is composed of at least five male-specific lethal (msl) genes and two non-coding RNAs. In the present study, we cloned all five homologs (termed the msls) from the silkworm, Bombyx mori, in which the issue of dosage compensation remains controversial. Data mining and robust phylogenetic analysis revealed that msls are ubiquitously present in genomes, from insects to mammals, with unknown biological functions. However, the five genes seem to have evolved at different times due to gene duplication. Real-time reverse transcriptase-polymerase chain reaction experiments demonstrated that in the silkworm, some Z-linked genes are expressed at higher levels in females than in males while others are expressed at lower levels in females than in males, which suggests that the msl genes in the silkworm and fruit fly may have different roles. We discussed the relationship of phylogeny between msl and sex-lethal.
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