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Genetic Variations of Plasma and Red Cell Proteins in Cheju Native Horse
Oh, Moon You,Kim, Ki Ok,Kim, Se Jae,Ko, Mi Hi,Choung, Chang Cho,Kim, Kyu Il 한국유전학회 1990 Genes & Genomics Vol.12 No.4
The genetic variations of the ten proteins in plasma and red cell were studied by the gel electrophoretic methods in Cheju native horse. Among the 10 proteins analysed in this study, 7 proteins (A1, Es, Pi, Hb, 6-PGD, PGM, Tf) were very polymorphic, while 3 proteins (Hp, MDH, ME) were monomorphic. The alleles showing the highest frequency in 7 proteins were A1B(0.602), Es1 (0.712), PiL(0.491), TfF(0.674), HbB1(0.620), 6-PgdF(0,823), PgmF(0.434), respectively.
Mi Hyune Oh,Moo Song Lim,Jeung Young Chai,Eun Jung Kim,Joong Hoon Cho,Chul Joo Lim,Sun Ok Choi 한국식품위생안전성학회 2017 한국식품위생안전성학회지 Vol.32 No.2
에너지 음료는 카페인을 주성분으로 타우린, 비타민 같은 다른 energy-enhancing 성분을 함유하고 있다. 미국과 유럽에서는 글루쿠로노락톤이 에너지 음료에 첨가될수 있으나, 국내에서 의약품으로는 허가되어 있다. 따라서 식품 첨가물로는 그 사용이 금지 되어 있어, 지속적으로 수입 및 유통 음료에서 시험검사를 하여 규제하고 있다. 현재 분석법으로 사용하는 LC-PDA 법은 복잡한 유도체화 과 정을 거치고, 음료 중에 당류들이 위양성 결과를 나타내 기도 한다. 이런 기존 방법의 단점을 개선하기 위해 HILICESI- MS/MS (hydrophilic interaction liquid chromatography coupled to electrospray ionization tandem mass spectrometry) 를 이용한 분석법을 개발하고, 선택성, 직선성, 검출한계, 정량한계, 정밀도, 정확성, 재현성에 대하여 분석법 유효성 검증을 수행했고, AOAC, EURACHEM 가이드라인에 부합되는 결과를 얻었다. A rapid, sensitive analytical method for glucuronolactone in beverages was developed and validated using hydrophilic interaction liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HILIC-ESI-MS/MS). To determine the optimum analytical conditions for glucuronolactone, three different kinds of HILIC columns and two mobile phases with different pH values were examined. An amide-bonded stationary phase with a pH 9 acetonitrile-rich mobile phase was the best condition in terms of column retention, ESI-MS/MS response area, and signal-to-noise ratio. After extraction, glucuronolactone was separated through the HILIC amide column and detected by negative ESI-MS/MS in selected reaction monitoring (SRM) mode. Nine energy drinks sold in Korea were spiked with glucuronolactone at a concentration of 5 ng/mL; the Monster EnergyTM sample showed the smallest peak area and its signal-to-noise ratio was used for method validation. Good linearity was obtained in the concentration range from 20 to 1500 ng/mL with a correlation coefficient > 0.998. The developed method had a limit of detection (LOD) of 6 ng/mL and a limit of quantitation (LOQ) of 20 ng/mL. The recovery of this method at concentration of 20, 100, 500, and 1000 ng/mL was 96.3%-99.2% with relative standard deviations (RSD) of 1.6%- 14.0%. A reproducibility precision assessment at concentration of 100 and 500 ng/mL was carried out among three laboratories. The recovery of that evaluation was 95.1%-102.3% with RSD of 2.7%-7.0%. An analysis of variance indicated that there was no difference between the recovery results of the three laboratories at the 5% significance level. The validated method is applicable to inspecting beverages adulterated with glucuronolactone in Korea.
Mi Kyeong Lee,이기용,Junghyun Park,김승현,Ok-Gyung Choi,Jin-Ho Park,Jung Hee Cho,Do Hoon Kim,Ju Hyun Baek,Mi Hyune Oh,김효진,Sang Hyun Sung 한국생약학회 2010 Natural Product Sciences Vol.16 No.1
High performance liquid chromatographic method with diode-array detection (HPLC-DAD) has been performed for the simultaneous determination of four marker constituents, paeoniflorin, trans-cinnamic acid, schisandrin and glycyrrhizin in traditional herbal medicinal preparation, So-Cheong-Ryong-Tang (SCRT). The presence of paeoniflorin, trans-cinnamic acid, schisandrin and glycyrrhizin in this decoction was ascertained by retention time, spiking with each authentic standard, UV spectrum and ESI mass spectrum. All four compounds showed good linearity (r2 > 0.998) in a relatively wide concentration ranges. The RSD for intra-day and inter-day precision was less than 3% and the limits of detection (LOD) were less than 30 ng. The mean recovery of each compound was 94.1 - 113.0% with RSD values less than 3.0%. These results suggest that the developed HPLC method is simple, effective and could be readily utilized as a quality control method for commercial SCRT products.
Oh, Moon You,Ko, Mi Hee,Jung, Yong Hwan,Oh, You Sung,Kim, Gi Ok,Kim, Moon Hong 한국유전학회 1997 Genes & Genomics Vol.19 No.4
We analyzed the phylogenetic relationship among eight species (P. pendula for. asendens Ohwi, P. yedoensis Matsumura, P. sargentii Rehder, P. serrulata var. quelpaertensis Uyeki, P. maximowiczii Rupr., P. serrulata var. pubescens Nakai, P. buergeriana Miquel, P. serrulata var. spontanea Wils.) of genus Prunus in Mt. Halla and cultivated P. yedoensis Matsumura using random amplified polymorphic DNA(RAPD) analysis. RAPDs were identified in nine species by amplification using single 10-mer primers of arbitrary sequence. Nine species were clearly classified with 8 arbitrary random primers which generated 76 polymorphic amplified DNAs or RAPDs. The phylogenetic tree was constructed from the RAPD fragments patterns by neighbor-joining method. The genetic distance between Prunes sargentii Rehder and P. serrulata var. quelpaertensis Uyeki was 0.3036 and was the lowest than those of any other pairs, the other side, between P. yedoensis Matsumura and P. yedoensis Matsumura-Cultivar was 0.4297. These results showed that RAPD analysis is a useful tool or elucidating phylogenetic relationship among 9 species of cherry trees.
Anti-inflammatory effect of resveratrol by inhibition of IL-8 production in LPS-induced THP-1 cells.
Oh, You-Chang,Kang, Ok-Hwa,Choi, Jang-Gi,Chae, Hee-Sung,Lee, Young-Seob,Brice, Obiang-Obounou,Jung, Hyun Ju,Hong, Seung-Heon,Lee, Young-Mi,Kwon, Dong-Yeul Institute for Advanced Research in Asian Science a 2009 The American journal of Chinese medicine Vol.37 No.6
<P>Resveratrol is a polyphenol compound and prominent anti-inflammatory agent found in plants, including the fruits of Morus alba. However, the therapeutic mechanisms of resveratrol remain largely unclear. To gain insight into the biological effects of resveratrol, we examined its influence on LPS-induced IL-8 production in the human monocytic cell line, THP-1. In inflammatory diseases, IL-8 plays a central role in the initiation and maintenance of inflammatory response. In the present study, IL-8 production was measured by ELISA and RT-PCR, while MAPK activation, IkappaBalpha degradation, nuclear factor (NF)-kappaB activation and cyclooxygenase (COX)-2 expression were determined by Western blot analysis. Resveratrol inhibited LPS-induced IL-8 production in a dose-dependent manner. Furthermore, resveratrol inhibited extracellular signal-regulated kinase (ERK) and p38 MAPK phosphorylation, IkappaBalpha degradation, NF-kappaB activation and cyclooxygenase (COX)-2 expression, which suggest that resveratrol inhibits IL-8 secretion by blocking MAPK phosphorylation and NF-kappaB activation. Taken together, these findings may help elucidate the mechanism by which resveratrol modulates THP-1 cell activation under inflammatory conditions.</P>
Oh, Soon-Ok,Jeon, Hae-Sook,Lim, Kwang-Mi,Koh, Young-Jin,Hur, Jae-Seoun The Korean Society of Plant Pathology 2006 Plant Pathology Journal Vol.22 No.4
Antifungal activity of Korean and Chinese lichen-forming fungi(LFF) was evaluated against plant pathogenic fungi of Botryosphaeria dothidea, Botrytis cinerea, Diaporthe actinidiae, Pestalotiopsis longiseta, Pythium sp., Rhizoctonia solani, and Sclerotium cepivorum. The LFF were isolated from Cladonia scabriuscula, Melanelia sp., Nephromopsis asahinae, Nephromopsis pallescens, Parmelia laevior, Pertusaria sp., Ramalina conduplicans, Ramalina sinensis, Ramalina sp., Umbilicaria proboscidea and Vulpicida sp. with discharged spore method. The isolates were deposited in the herbarium of Korean Lichen Research Institute(KoLRI) in Sunchon National University. The LFF of Melanelia sp., P. laevior, Pertusaria sp., R. conduplican and Ramalina sp. exhibited strong antifungal activity against all of the pathogenic fungi examined. Among them, LFF of P. laevior showed more than 90% of inhibition in fungal hyphae growth, compared with control. The results imply that LFF can be served as a promising bioresource to develop novel biofungicides. Mass cultivation of the LFF is now under progress in laboratory conditions for chemical identification of antifungal substances.