Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

대학생의 성의식 실태에 관한 연구

강미라,김두화,김성현 濟州大學校 學生生活硏究所 2005 學生生活硏究 Vol.26 No.-

This research was conducted to help college students better understand the culture on sex and provide basic data that can be usefully used for education on sex in the future by exploring college students: experience and perception on sex. 964 participated in this survey. 425 were female, and 539 male. Results of the survey are as follows. 1. Knowledge About Sex First, when asked about education on sex, 92.4% of respondents said they received education on sex, and its focus was primarily placed on venereal diseases and contraception. Female students received more of the education. While men learned more about sex, women were educated relatively more about abortion. Second, when questioned about the benefits of education on sex, over 40% of respondents responded neutrally to the benefits of education on sex, which means that they believe the education helps little. Third, in terms of knowledge about sex, participants know best about pregnancy. On the other hand, they have least knowledge about venereal diseases. By sex, male participants answered they know better in five categories than their counterparts. Female respondents received more education, but female participants had more knowledge, so more detailed research are necessary to explore how accurate their knowledge is. Fourth, in most cases, they get knowledge from school (29.2%), and friends or older students (19.0%). Concerns are raised about the quality of information acquired from friends or older students. Fifth, a majority of participants (54.9%) get knowledge about sex while in middle school. some of them (26.8%) while in elementary school. It is partly because children mature earlier than before, and they are exposed to a variety of media, increasing chances of getting misleading information on sex. 2. Opinions on Marriage First, when asked about marriage, over 30% of participants said that they 'like living alone if possible' or 'it depends.' By sex, while males have positive opinions on marriage, females reserve their comments. Second, in terms of having sex before marriage, 70% supported it. By sex, 30.3% of female respondents said having sex before marriage is not right, and only 18% of male participants said it is not right. It can be referred that females are more conservative. Third, when asked about living together without getting married, only 4.7% of participants had negative opinions. Most participants said it is acceptable. By sex, males were more tolerant than their counterparts. Fourth, in terms of virginity, 70% are open. Males are more open than females. Fifth, when asked about how they react if their mates raise questions of virginity, 54.2% of male respondents said they will confess and ask forgiveness. On the other hand, 21.3% of female participants said they will break up, and 35.1% had other choices. The fact shows that women tend not to face the problem, and act passively. Sixth, when questioned about how they would respond if they know their mates are not virgin, more than half of male respondents said it does not matter. More males said it does not matter than females, and at the same time, male respondents who said they will break up outnumbered their counterparts. This shows that male participants have both open and conservative attitudes towards virginity. Seventh, when asked whether their mates can meet friends with the opposite sex after marriage, 8.3% said 'no,' and 91.7% said 'yes.' Most student are tolerant about mates can meet friends with the opposite sex after marriage. By sex, men were more cautious than women. Eighth, 70% of respondents shows negative opinions about extramarital sex. 3. Dating First, in terms of sexual experience, 70% of respondents said they had no experience. By sex, men had more sexual experience than women. Second, most of the respondents who have sexual experience tend to make an emotional decision. By sex, men are more impulsive than women. Third, the longer relationship continues, the higher chances of having sex there are. By sex, in terms of women, chances of having sex increase as relationship continues longer. On the other hand, men are more impulsive. Fourth, when it comes to psychological status when having sex, both sexes are relatively satisfied. Fifth, 53.7% tend to continue their relationship after having sex. Sixth, both sexes are satisfied after having sex, however women are particularly worried about pregnancy. 4. Sex-related Problems First, 46.4% of respondents said they currently have no sex-related worries, and 22.7% have problems related to the opposite sex. Men are worried about impulsiveness, and women are worried about a physiological phenomenon. Second, when it comes to solutions, 46.9% said they consult with their friends. Only 0.7% said they meet with their teacher. Apart from discussing it with their friends, women opt for relationship-oriented solutions, consulting with their parents. On the other hand, men prefer object-oriented solutions, gathering information through friends, books, and the Internet. Based on these results, suggested advice is as follows: First, college students have received education on sexual morality since middle school, and they collect information primarily from their friends or older students. But most of them are dissatisfied with the benefits of the education. By sex, women received more education, but they have less knowledge than men. Therefore, it is necessary to review how effective education on sexual morality conducted in school is. Second, more than half of students have positive opinions on marriage, and they are open about having sex or living together before marriage, and meeting friends with the opposite sex. In the past, the marriage system itself was considered important, but in modern society, love tends to be put before marriage. Therefore, It is necessary to develop a variety of educational and cultural events to bring about desirable sex morality. Third, most of respondents are having a date, but not many of them have sex experience. respondents who have sex experience tend to make an impulsive decision without thinking carefully, continuing their relationship longer. Therefore, there should be some opportunities to help them make a desirable decision on sex.

임플란트의 collar design이 변연골과 연조직에 미치는 영향

유현상,강선녀,정창모,윤미정,허중보,전영찬 대한치과보철학회 2012 대한치과보철학회지 Vol.50 No.1

연구 목적: 임플란트 경부가 잘 설계된 경우 양호한 연조직 반응을 통해 변연골을 보존하는데 도움이 된다. 본 실험에서는 연, 경조직 경계부에 가장 가까이 위치하는 임플란트의 collar design이 변연골 변화와 연조직 반응에 미치는 영향을 동물실험을 통해 알아보고자 하였다. 연구 재료 및 방법: 2마리의 건강한 Beagle dog에 임플란트 collar design만 다른 두 종류의 임플란트(Neobiotech Co. Seoul, Korea)를 식립하였다. Collar에 bevel 을 부여한 군(Bevel 군)과 "S"자 형태를 부여한 군(Bioseal 군)으로 나누어 마리 당 7개, 군당 7개, 총 14개의 임플란트를 무작위로 식립한 후 Healing abutment를 즉시 체결하였다. 디지털 표준구내 방사선사진을 이용해 4주 간격으로 총 12주간 근원심 변연골 변화를 관찰하였고, 12주에 희생하여 조직학적 분석을 통해 협설 변연골 흡수 및 임플란트 주변 연조직 반응을 평가하였다. Mann-Whitney test를 통해 동일한 방사선 사진 촬영 시점에서 근원심 변연골 변화량 및 조직계측치를 군 간 비교하였고, Kruskal-Wallis test를 통해 방사선 사진상 근원심 변연골 변화량이 시간에 따른 차이가 있는지 군 내 분석 한 후 Duncan test를 통해 사후 검증하였다(α=.05). 결과: 방사선학적 분석 결과 각 촬영 시점에서 두 군간 근원심 변연골 변화량의 차이를 보이지 않았다(P>.05). 군 내에서 시간에 따른 근원심 변연골의 흡수량을 분석한 결과 Bevel 군에서는 시간에 따른 차이를 보이지 않았으나 (P>.05), Bioseal 군에서는 시간에 따른 차이를 보였으며, 4주및8주와 비교했을 때 12주에서 변연골의 증가를 보였다(P<.05). 조직학적 분석 결과 협설측 변연골 흡수량에서 두 군간 차이를 보이지 않았으나 (P>.05), Bevel 군에 비해 Bioseal 군에서 더 견고한 결합조직부착을 관찰할 수 있었으며, 생물학적 폭경의 값은 두 집단 간 차이를 보이지 않은 반면에 (P>.05), 접합상피부착은 Bevel 군에서 유의하게 길었고, 결합조직부착은 Bioseal 군에서 더 길게 나타났다(P<.05). 결론: Bevel 군에 비해 Bioseal 군에서 결합조직부착은 길게 형성된 반면에 접합상피부착은 더 짧게 나타났으며, 생물학적 폭경과 초기 변연골 흡수에는 차이가 없음을 알 수 있었다. 연조직 반응의 차이가 실제 기능하중 하에서 변연골 변화에 미치는 영향에 대해서 향후 연구가 필요할 것으로 생각된다. PURPOSE. The purpose of this study was to investigate the effects of implant collar design on marginal bone change and soft tissue response by an animal test. MATERIALS AND METHODS. Two types of Implant (Neobiotech Co. Seoul, Korea) that only differs in collar design were planted on two healthy Beagle dogs. The implants were divided into two groups, the first group with a beveled collar (Bevel Group) and the second group with "S" shaped collar (Bioseal group). Standardized intraoral radiographs were used to investigate the mesio-distal change of the marginal bone. Histological analysis was done to evaluate the bucco-lingual marginal bone resorption and the soft tissue response adjacent to the implant. Mann-Whitney test was done to compare the mesio-distal marginal bone change at equivalent time for taking the radiographs and the tissue measurements between the groups. RESULTS. Radiographic and histological analysis showed that there was no difference in marginal bone change between the two groups (P>.05). Histological analysis showed Bioseal group had more rigid connective tissue attachment than the Bevel group. There was no difference in biological width (P>.05). Bevel group showed significantly longer junctional epithelium attachment and Bioseal group showed longer connective tissue attachment (P<.05). CONCLUSION. For three months there were no differences in marginal bone change between the Bevel group and the Bioseal group. As for the soft tissue adjacent to the implant, Bioseal group showed longer connective tissue attachment while showing shorter junctional epithelium attachment. There were no differences in biologic width.

급성 췌장염을 동반한 임신성 급성 지방간 1 예

강미선;박상훈;박지영 서희영;허란;김상현 인제대학교 백병원 2011 仁濟醫學 Vol.32 No.-

Acute fatty liver of pregnancy (AFLP) was first described as a specific clinical entity in 1940 and thought to be universally fatal. Maternal mortality in the past approached 75 percent. However early diagnosis and prompt delivery have dramatically improved the prognosis, and maternal mortality rate is now less than 18 percent. A early diagnosis, a prompt delivery, and a intensive supportive care have improved maternal and perinatal outcome. We report a 30—year—old woman at 37 weeks gestation who have multiple complications including acute pancreatitis in AFLP. She was complicated with acute renal failure, disseminated intravascular coagulation, duodenal ulcer bleeding, spontaneous bacterial peritonitis, pulmonary edema and acute pancreatitis.

감각처리와 관련된 섭식기능의 평가와 중재방법에 대한 고찰

강현진,김경미 대한감각통합치료학회 2010 대한감각통합치료학회지 Vol.8 No.1

섭식기술의 문제는 성장, 학습, 의사소통, 다른 아동과의 상호작용 등 삶의 모든 면에 영향을 줄 수 있다. 구강섭식은 입을 열고 음식/액체를 입속에 넣고 삼키는 것이다. 본 문헌연구의 목적은 아동의 섭식과 관련된 평가방법과 중재방법에 대해 알아보는 것이다. 섭식은 아동 초기 발달의 중요한 요소이며 일상생활 참여를 촉진한다. 아동이 음식을 먹을 때 받아들이는 방법에 차이가 있다. 그 이유로는 음식으로부터 제공되는 감각자극의 등록과 해석의 과정을 거쳐 섭식행동으로 나타나기 때문이다. 감각처리 문제로 인한 섭식의 문제는 촉각과민성, 구강거부, 촉각과소반응, 구강실행증으로 분류할 수 있다. 섭식문제의 평가 가운데 감각처리 과정에서 나타날 수 있는 문제들을 확인하고 중재계획을 세우기 위해서는 섭식과 관련된 신경학적 과정을 이해하고 섭식과 관련된 감각처리의 특성을 아는 것이 중요하다. 또한 감각처리와 관련되어 야기된 섭식문제의 중재방법을 고찰하는 것은 의의가 있으며, 이는 섭식에 관한 효율적인 접근을 통해 아동들에게 보다 나은 삶을 제공할 수 있으리라 기대된다. Problems in feeding habits could affect various aspects of children including growth, learning, communication, interaction with other children, etc. Oral consumption was defined as a participant opening his/her mouth, accepting food/liquid, and swallowing. A goal of the study is to find a method of evaluation and intervention of a child's feeding habits. Feeding is an important component in the early development of children and may have later consequences in the child's ability to participate successfully in their activities of daily life. Children show personal difference on the foods which they try first because stimuli from the foods are revealed as a mealtime behavior through sensory registration and processing. Feeding problems in sensory processing consist of tactile oversensitivity, oral refusal, tactile undersensitivity, and oral dyspraxia. In order to identify problems of sensory processing among feeding difficulties and plan intervention, the understanding of neurological processing and sensory processing characteristics related to feeding is necessary. Reviewing intervention for feeding problems related to sensory processing is required for an effective approach for feeding and providing better life for children.

여대생의 결혼관에 관한 연구

강신미,김욱련,김이화,김현주,백은주 효성여자대학교 가정대학 학도호국단 1985 家政大論集 Vol.4 No.-

In these days, Korea has undergone various changes in every social aspect throughthe process of modernization. Especially in instituional and actual aspects, the family.and marriage have been changed greatly by the rapid urbanization and iudustrializatton,.and these changes have been investigated scientifically. This paper presents marital consciousness and actual behavior of 300 woman collegestudents in Daegu, and was surveyed comparing various parts-the humanities, natural·science and the arts. As for the basic attitude of marriage, they were positive in the need of marriage,,defined marriage as the second starting point of life. Also they put the aim of marriage,on mental value. As for the dating and purity before marriage, 84 percent of respondents assented tothe dating before marriage. And they thought it proper that the duration of datingwas one year to two years. The age of the first dating and the standard of sex moralstore significant. The part of the arts tended to be open, and the part of natural sciencewere conservative. As for the standard of spouse's choice, 84 percent of respondents wanted to have theright of priority they could choose their spouses for themselves. And they thought it·proper that the age of marriage was 25-year-old to 26-year-old, and the gap of maritalage was one year to three years. Ideal spouse's characteristic, home surroundings, and marital harmony were significant. Among various marital conditions, personality tended to to emphasized, and theatmosphere of family and masculine appearance were taken serious. As for a. spouses's job, professor or research occupation was popular. Compared withthe part of the arts, the part of the humanities and natural science were positive inpredicting marital harmony. Among various marital conditions, an only son, the eldest son, and an offspring ofa concubine tended to be evaded. The type of an ideal spouse was companionship.

태극권 수련이 중년여성의 심리적 행복감에 미치는 영향

강현숙,김백윤,박미진,신영호 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.2

The purpose of this study was to explore the effects of Taichichuan training on the variables of cognitive-affective states. The subjects of the study consisted of 22G middle aged women in Jeonju areas. The survey methods of this research are the 5 Likert Scale modified the Cognitive Affective States Scale by Yang. Myung-Hwan(1998). The used statistical methods for the data analysis were M. SD. Paired sample T-Test. The results obtained by the appropriate analytic processes were followed. 1. In hedonic enjoyment, Taichichuan training women were lower than non-activity women. 2. In feeling of flow, Taichichuan training women were higher than non-activity women. 3. In self-realization, Taichichuan training women were higher than non-activity women. 4. In self-confidence, Taichichuan training women were higher than non-activity women.

공동주택 사전성능평가를 위한 성능평가 시스템 개발에 관한 연구

강미선,이상현,김하나,엄혜진 대한건축학회 2003 대한건축학회논문집 Vol.19 No.10

The purpose of this study is to propose an evaluation framework for Collective Housing and to develop the Design Evaluation System. The Performance Evaluation System of Collective Housing in the Design Development Stage evaluates its design results by running CAD program. The results are automatically reflected in the design process. The scope of this study will be limited to the contents within the Barrier Free Design. Running the design evaluation program requires the conversion of design forms and design checklist to computerized ones. To efficiently conduct this process, First, organize the existing guideline of the Barrier Free Design. Second, define attributes of each part in Building Data Model with the analysis of the checklist. Third, develop the Performance Analysis agent that compares Building Data Model with translated checklists. Finally, verify the efficiency of the Performance Evaluation System by seeking experts' advice on the result of the program.

해운대 달맞이 언덕에 있어서 바다조망경관의 역사적 변천에 관한 연구

강영조,신수안,조미나,이현주,박은희 동아대학교 건설기술연구소 2000 硏究報告 Vol.24 No.1

The purpose of this study is to clarify the historic transition of the prospects on Haeundae dalmaji hill. Dalmaji hill is well known for a character of configuration on the ground with the scene of the sea. We will infer that how did it change the prospects of Dalmaji hill's sea through the analyzing of the preset situation and location of building on the hill. (1) We investigated the stories of the building, the history, the business condition. (2) We collect the twenty four view points which is able to have seascape on the hill, being easily approaching to the road, and condition of changing the building. (3) We clarified the transition of seascape prospect and considered the characteristics of the six types from which were divided the condition of seascape prospect.

난원공 개존을 통한 우심방에서 좌심방으로의 단락 진단에 있어 경흉부 심초음파 하모닉 영상의 유용성

신미승,강석민,장길진,변기현,하종원,정남식,허지회,이병인 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4