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Small nuclear RNA variants of three Bombyx mori strains
Annia MESA,Jason A. SOMARELLI,Rene J. HERRERA 한국곤충학회 2008 Entomological Research Vol.38 No.1
The spliceosome is a high molecular weight cellular complex responsible for the removal of non-protein coding introns from pre-mRNA to form mature mRNA transcripts. It comprises five major uridine (U)-rich small nuclear (sn)RNAs, to which a number of proteins bind and interact. Variant snRNAs have been identified in several organisms, although it remains to be seen whether or not these isoforms have distinct cellular roles. Nevertheless, many of these sequences have spatiotemporal trends in expression, suggesting that the variant snRNAs are not functionally equivalent. In this report, we examine and contrast the available data on snRNAs in two strains of Bombyx mori : European 703 and Nistari from India. In addition, the genomic snRNA sequences from the p50T strain are described. Thus far, isoforms of U1, U2, U4 and U6 have been characterized in B. mori European 703 and/or Nistari strains using expression libraries. In this study, an in silico approach was used to identify the genomic counterparts of the U snRNA variants in the 6X Whole Genome Shotgun (WGS) of the B. mori p50T strain. The present study is the first comparison of snRNAs in different B. mori strains. Overall, we found that 46 full length U snRNA loci and 76 suspected truncated genes are present in the B. mori genome of the p50T strain. A total of 14 full length genes match previously identified snRNAs in either the Nistari and/or the European 703 strains. Multiple sequence alignments of upstream controlling elements revealed conserved boxes in a subset of U snRNA genes. The presence of divergent promoters within specific snRNA 5′-flanking sequences suggests that these loci may be transcribed from different controlling elements or are not expressed. The number of nucleotide differences within a given type of U snRNA is strongly correlated with its copy number in the genome (r2= 77.8%) and it may reflect a relaxation of selection pressure on genes of higher copy number. The multiplicity in gene copy may provide for numerous, full length snRNA loci with variable sequences that adopt unique roles in pre-mRNA splicing, possibly by modulating protein–NA and/or RNA–NA interactions and in doing so affecting gene expression and development.
Jaime A. Mesa,Iván Esparragoza,Heriberto Maury 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.6 No.2
The purpose of this paper is to perform the analysis of literature review regarding the design of open architecture products (OAP) and their potential benefits within the circular economy (CE) model. The analysis involved studying more than 80 research articles during the last two decades in engineering journals. The articles were gathered through a bibliometric analysis using the most relevant keywords concerning product design, sustainability, OAP, and CE. Main trends, challenges and future scopes of research opportunities and development were identified. The study provides a framework to designers and researchers involved in the design of OAP to enhance their sustainability performance for a CE model, which integrates lifecycle considerations (reuse, remanufacturing, repair, and recycle), resource optimization, and emissions reduction. The findings include the need for design methods focused on the design of OAP to guarantee an effective circularity of resources during the whole lifecycle of products and the need of integrating manufacturing processes and material analysis to design products capable of adapting to the CE model.
Florencia Anabel Mesas,Marí,a Cecilia Terrile,Marí,a Ximena Silveyra,Adriana Zuñ,iga,Marí,a Susana Rodriguez,Claudia Anahí,Casalongué,Julieta René,e Mendieta 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.6
Chitosan has been considered an environmental-friend- ly polymer. However, its use in agriculture has not been extended yet due to its relatively low solubility in water. N-Methylene phosphonic chitosan (NMPC) is a water- soluble derivative prepared by adding a phosphonic group to chitosan. This study demonstrates that NMPC has a fungicidal effect on the phytopathogenic fungus Fusarium solani f. sp. eumartii (F. eumartii) judged by the inhibition of F. eumartti mycelial growth and spore germination. NMPC affected fungal membrane per- meability, reactive oxygen species production, and cell death. Also, this chitosan-derivative exerted antifungal effects against two other phytopathogens, Botrytis cine- rea, and Phytophthora infestans. NMPC did not affect tomato cell viability at the same doses applied to these phytopathogens to exert fungicide action. In addition to water solubility, the selective biological cytotoxicity of NMPC adds value in its application as an antimicrobial agent in agriculture.
Parents as Advocates : The Phililppine A Case Study
Teresita G. De Mesa 대구대학교 2002 Journal of Asia-Pacific Special Education Vol.2 No.1
This case study documents the collaborative partnership of parents and professionals to effect a quaility program for children with mental handicap. It also describes how the community and its resources can also be harnessed toward providing full participation and quality of opportunity to these children. The Parent Council of St, John Maria Vianney has launched a pilot project called parents as Advocates: A Case Study. The study dealt on the multifarious roles the parents take on as advocates trsilting in creating as awareness among other parents and the community. Thirty(30)parents were organized as a team and merged themselves in various project of the schools and the city. They assumed varous roles such as home teachers, teacher aides, facilitatirs, peer tutors, mentors. resource person, lobby groups, organizers and other similar activities.
Ciro, J.,Mesa, S.,Uribe, J.,Mejia-Escobar, M.,Ramirez, D.,Montoya, J.,Betancur, R.,Yoo, H. S.,Park, N. G.,Jaramillo, F. Royal Society of Chemistry 2017 Nanoscale Vol.9 No.27
<P>Effective control of the interface between the metal cathode and the electron transport layer (ETL) is critical for achieving high performance p-i-n planar heterojunction perovskite solar cells (PSCs). Several organic molecules have been explored as interlayers between the silver (Ag) electrode and the ETL for the improvement in the photovoltaic conversion efficiency (PCE) of p-i-n planar PSCs. However, the role of these organic molecules in the charge transfer at the metal/ETL interface and the chemical degradation processes of PSCs has not yet been fully understood. In this work, we systematically explore the effects of the interfacial modification of the Ag/ETL interface on PSCs using rhodamine 101 as a model molecule. By the insertion of rhodamine 101 as an interlayer between Ag and fullerene derivatives (PC60BM and PC70BM) ETLs improve the PCE as well as the stability of p-i-n planar PSCs. Atomic force microscopy (AFM) characterization reveals that rhodamine passivates the defects at the PCBM layer and reduces the band bending at the PCBM surface. In consequence, charge transfer from the PCBM towards the Ag electrode is enhanced leading to an increased fill factor (FF) resulting in a PCE up to 16.6%. Moreover, rhodamine acts as a permeation barrier hindering the penetration of moisture towards the perovskite layer as well as preventing the chemical interaction of perovskite with the Ag electrode. Interestingly, the work function of the metal cathode remains more stable due to the rhodamine incorporation. Consequently, a better alignment between the quasi-Fermi level of PCBM and the Ag work function is achieved minimizing the energy barrier for charge extraction. This work contributes to reveal the relevance of proper interfacial engineering at the metal-cathode/organic-semiconductor interface.</P>
Implementation and Evaluation of an HMM-Based Speech Synthesis System for the Tagalog Language
김경태,김종진,Mesa, Quennie Joy,Kim, Kyung-Tae,Kim, Jong-Jin 대한음성학회 2008 말소리 Vol.68 No.-
This paper describes the development and assessment of a hidden Markov model (HMM) based Tagalog speech synthesis system, where Tagalog is the most widely spoken indigenous language of the Philippines. Several aspects of the design process are discussed here. In order to build the synthesizer a speech database is recorded and phonetically segmented. The constructed speech corpus contains approximately 89 minutes of Tagalog speech organized in 596 spoken utterances. Furthermore, contextual information is determined. The quality of the synthesized speech is assessed by subjective tests employing 25 native Tagalog speakers as respondents. Experimental results show that the new system is able to obtain a 3.29 MOS which indicates that the developed system is able to produce highly intelligible neutral Tagalog speech with stable quality even when a small amount of speech data is used for HMM training.