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        A Hydrophilic/Hydrophobic Janus Membrane Used as Wound Dressings with Enhanced Antibacterial Properties

        Mengqi Song,Qian Zhao,Xiuting Wang,Chuan Shi,Xiao Hu,Jiwei Li 한국섬유공학회 2022 Fibers and polymers Vol.23 No.9

        In this study, an asymmetric wettable composite wound dressing consists of hydrophobic polyvinylidene fluoride(PVDF) outer layer and a hydrophilic polydopamine (PDA) coated Ag nanoparticles (Ag NPs)/PVDF layer (PVDF-Ag-PDA) was successfully prepared through electrospinning and PDA coating. The results show that the contact angle of thePVDF surface is 132.8 °, while the PDA-coated surface is nearly 0 °, indicating an asymmetric wettability. The highlyhydrophilic PVDF-Ag-PDA layer can significantly reduce bacterial growth, while the PVDF layer with a small pore size andhigh hydrophobicity can prevent bacterial adherence. In addition, cytotoxic assays showed that PVDF/PVDF-Ag-PDApresent a high cell viability percentage (above 80 %), which is attributed to that the thin layer of PDA can alleviate thecytotoxicity of Ag NPs. Therefore, the Janus wound dressing noted as PVDF/PVDF-Ag-PDA has the potential for practicalapplications with the advantages mentioned above.

      • KCI등재

        Basement Design for Vibration Reduction of High-Rise Buildings under Metro Operation

        Zhaowei Chen,Song Peng,Defeng Zeng,Qinglie He,Jing Tang,Mengqi Zhang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.4

        Vibration induced by metro operation on the underground line is transmitted to the buildings above the line, which adversely affects on the normal life and work of human in the building. To study this problem, based on the plate-shell vibration theory, a plate-column-plate coupled model is constructed to analyze the influence of plate thickness, plate elastic modulus and column arrangement on the vibration in mechanism firstly. Relying on a practical engineering problem, two models are established, namely a train-track coupled dynamic model and a monolithic track bed-tunnel-soil-building model, loading fastener force from the train-track coupled dynamic model on the monolithic track bed, vibration propagates to high-rise building through soil-building coupling. Field test is conducted to verify the reliability of the numerical model. On the basis, the vibration law of building under different basement floor thicknesses, basement floor material and arrangement of non-permanent columns are studied. Results show that changing the basement design parameters has different effects. The Z vibration level of building floor decreases with the increase of basement floor thickness. Basement floor thickness increases by 0.02 m, the Z vibration level of the floor decreased by 1.4 – 2.2 dB at most. The decrease of elastic modulus leads to a slight decrease of the Z vibration level of building, the change is less than 1 dB. Without changing the arrangement of basement load-bearing columns, reducing non-permanent structural columns significantly reduces the Z vibration level, with attenuation of up to 5.1 dB.

      • KCI등재

        The Chitin-Induced Chimeric LYK4-ER Gene Improves the Heat Tolerance of Arabidopsis at the Seedling Stage

        Linxiao Chen,Wei Xia,Jinxing Song,Mengqi Wu,Zhizhen Xu,Xiangyang Hu,Wenqing Zhang 한국식물학회 2020 Journal of Plant Biology Vol.63 No.4

        Due to global warming, high temperature has become the main abiotic stress affecting plant growth worldwide. LysM-containing receptor-like kinase 4 (LYK4) is the receptor for chitin, and ERECTA(ER) is a key factor in plant tolerance to high temperature. In this study, we constructed a chitin-induced chimeric LYK4-ER gene, in which the extracellular region and transmembrane domain of the LYK4 gene are fused with the intracellular region of the ER gene. Colony PCR, RT-PCR and western blot analyses of LYK4-ER transcription in plants, confirmed that the LYK4-ER gene was successfully constructed and transferred into Arabidopsis. The LYK4-ER gene localized to the cytomembrane and cytoplasm in vivo because of the binding properties of the transmembrane domain of the LYK4-ER gene to the cell membrane. The transgenic plants showed a higher germination rate and germination index as well as a shorter mean germination time than the wild-type plants, indicating that the LYK4-ER gene increases the heat tolerance of Arabidopsis. The lower H2O2 content and relative electrolytic leakage of the transgenic plants showed that the status of these plants under heat stress was improved. UPLC-MS/MS was used to analyze the phytohormones content, which suggested that the transgenic plants exhibited improved heat tolerance through jasmonic acid signal transduction pathways.

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