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        Comparison of the comb cell structure of Apis dorsata (Hymenoptera: Apidae) in two areas of China

        Deng Shangkao,Huang Rong,Meng Qingxin,Yang Shunhua,Zhao Wenzheng,Tian Yakai,Dong Xia,Dong Kun,Gong Xueyang 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

        The comb cell structure of Apis dorsata in different ecoregions is rarely reported. This study compared the dif ferences in the comb cell structure of Apis dorsata from Menghai of Yunnan Province and Chengmai of Hainan Province, China. Accordingly, parameters such as brood comb thickness, the width of ten consecutive brood cells, cell diameter, depth, inclination angle, and the side length of the cell mouth were measured. The results showed that within the same area, the widths of ten consecutive cells and single-cell diameter in the 0 ◦ direction were significantly bigger than that in the 60 ◦ and 120 ◦ directions, however, there was no significant difference for the same between the 60 ◦ and 120 ◦ directions. The average width of ten consecutive cells and the single-cell diameter showed significant differences between the two areas. These were significantly bigger in Menghai than in Chengmai. The average thickness of the brood comb, cell depth, and side length of the cell mouth were also significantly higher in Menghai than in Chengmai, however, the cell inclination angle showed no regional difference. These differences in the comb cell structure of Apis dorsata may be due to the difference in the ecological environment of the two areas. This study revealed the structural characteristics of the comb cells of Apis dorsata in different areas of China, increasing our biological knowledge of this bee species.

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        Anaerobic Ammonium-Oxidizing Bacteria in Cow Manure Composting

        ( Tingting Wang ),( Lijun Cheng ),( Wenhao Zhang ),( Xiuhong Xu ),( Qingxin Meng ),( Xuewei Sun ),( Huajing Liu ),( Hongtao Li ),( Yu Sun ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.7

        Composting is widely used to transform waste into valuable agricultural organic fertilizer. Anaerobic ammonium-oxidizing (anammox) bacteria play an important role in the global nitrogen cycle, but their role in composting remains poorly understood. In the present study, the community structure, diversity, and abundance of anammox bacteria were analyzed using cloning and sequencing methods by targeting the 16S rRNA gene and the hydrazine oxidase gene (hzo) in samples isolated from compost produced from cow manure and rice straw. A total of 25 operational taxonomic units were classified based on 16S rRNA gene clone libraries, and 14 operational taxonomic units were classified based on hzo gene clone libraries. The phylogenetic tree analysis of the 16S rRNA gene and deduced HZO protein sequences from the corresponding encoding genes indicated that the majority of the obtained clones were related to the known anammox bacteria Candidatus “Brocadia,” Candidatus “Kuenenia,” and Candidatus “Scalindua.” The abundances of anammox bacteria were determined by quantitative PCR, and between 2.13 × 10<sup>5</sup> and 1.15 × 10<sup>6</sup> 16S rRNA gene copies per gram of compost were found. This study provides the first demonstration of the existence of anammox bacteria with limited diversity in cow manure composting.

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