Control Effects of Metabolites from Paecilomyces spp. on Aphids in Spot and Field Trials

Weisong XU,Meiying Hu,Guohua Zhong 한국응용곤충학회 2005 한국응용곤충학회 학술대회논문집 Vol.2005 No.05

Silicon Dioxide Particles Deposited in Vessels and Cartilage of the Femoral Head

Min Xu,Dan Peng,Meiying Qing 연세대학교의과대학 2014 Yonsei medical journal Vol.55 No.5

Silicosis had been considered for decades as an illness with manifestations of lung fibrosis due to inhalation of overconcentrated SiO2 dust. To the best of our knowledge,studies have yet to report SiO2 deposits in any other tissues and organs. In the present case, while performing bilateral artificial total hip arthroplasty for one patient, we found that the articular cartilage of the bilateral femoral head was black. Therefore, specimens thereof were sent for pathological examination. Pathologicalexamination (immunohistochemistry) and polarized light microscopy revealedthe presence of considerable brown, acicular, rhombic, and crumb-like crystals. The crystals were mainly composed of SiO2. SiO2 could deposit in vessels and femoral head cartilage via blood circulation.

Treatment of Volatile Organic Compounds from a Typical Waste Printed Circuit Board Dismantling Workshop by a Pilot-scale Biotrickling Filter

Dongqi Liao,Jianjun Li,Duanfang Sun,Meiying Xu,Taicheng An,Guo-Ping Sun 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4

A pilot-scale biotrickling filter (BTF) was designed to treat volatile organic compounds (VOCs) emitted from a typical waste printed circuit board (WPCB) pyrolysis workshop. Measured by gas chromatography-mass spectrometry (GC-MS), the main components of VOCs and their concentrations were benzene, toluene, chlorobenzene, ethyl-benzene, xylene, styrene, benzaldehyde, and trimethyl-benzene. The removal efficiencies of the BTF for these compounds ranged from 81.1 to 97.8% after 90 days of operation. The maximum elimination capacity of 25.94 g/m3 h was obtained with the inlet loading of 30.72 g/m3 fixed the fixed empty-bed residence time (EBRT) of 9.80 sec. Hazard ratio index based on threshold limit value for time weighted average (TLV-TWA) and VOCs concentrations indicated that the cancer risk of VOCs was significantly reduced after the BTF treatment. The microbial community analysis revealed initial inoculum and some emerging bacteria played crucial roles in the improvement of BTF performance with the biodegradation of this kind of VOCs by the polymerase chain reactiondenaturing gradient gel electrophoresis (PCR-DGGE) technique and pyrosequencing analyses indicated that proteobacteria phylum was the dominant in the BTF. All above results indicated that VOCs with multicomponent and fluctuant concentrations from a typical waste printed circuit board pyrolysis workshop were removed efficiently and in an environmentally friendly way by the biofiltration method.

Evaluation of the Performance of Structured Mixed Packing and Inert Packing Materials in Toluene Biotrickle-filtration

Duanfang Sun,Jianjun Li,Taicheng An,Meiying Xu,Guoping Sun,Jun Guo 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.5

Packing materials play a key role in waste gas treatment. Organic and inert packing materials have their disadvantages, which may be minimized by mixed packing. In this study, various operating conditions were applied to evaluate the performance of structured mixed packing and inert packing materials in toluene biotricklefiltration. Four biotrickle filters were packed with structured mixed packing materials, namely, ceramic pall rings,ceramic rashig rings, and lava rock. Their toluene removal capacity was studied for 217 day using a laboratory-scale reaction under various operating conditions. The key elimination capacity (removal efficiency > 95%) ranking of the biotrickle filters was as follows: Structured mixed packing (306.20 ± 7.90 g/m^3/h) > pall ring (156.71 ± 7.84 g/m^3/h)> rashig ring (153.31 ± 6.14 g/m^3/h) > lava rock (150.32 ±9.19 g/m^3/h). The structured mixed packing and inert packing resulted in excellent toluene-degrading biofilter performance under long-term operation. The structured mixed packing provided a more rapid startup rate and better process robustness than the inert packing did. The biotrickle filter with mixed packing materials had a high elimination capacity which makes it suitable for various real-life applications, whereas the capability of the inert packing material was more suitable for treating a steady low toluene load.

Bacterial Community Diversity and Functional Gene Abundance of Structured Mixed Packing and Inert Packing Materials Based Biotrickling Filters

Duanfang Sun,Jianjun Li,Taicheng An,Meiying Xu,Guoping Sun,Jun Guo 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.3

Packing is the most important factor in biofilter design. A structured mixed packing (SMP) material, combined with various organic and inorganic materials (mineral matter is 80.18 ± 0.48%, w : w), was constructed by ureaformaldehyde resin in order to minimize the disadvantages of these materials when used as stand-alone components. The performance of the toluene biotrickling filter (BTF)packed with SMP was compared with the other BTFs packed with a ceramic raschig ring, ceramic pall ring, and lava rock, respectively, for 217 day under various operating conditions. Real-time PCR and DGGE techniques were applied to reveal the gene coding for the toluene-degrading enzymes and the bacterial community structure in the BTFs. The toluene-degradation gene copies exponentially increased, and bacterial diversity significantly decreased with the improving elimination capacities of the BTFs. The overload and shutdown operations resulted in insignificant fluctuations in the toluene-degradation gene copies at equal levels as well as a slight variation in the bacterial community structures in the BTFs. Various putative toluenedegrading bacteria were found using sequencing bands from the DGGE gels; some bacteria, such as Burkholderia spp., were further confirmed by real-time PCR; other bacteria,such as Alcaligenes spp., might not have been reported. The packing properties of SMP material supported more toluene-degradation gene copies in the biofilm, and higher toluene-degrading bacterial diversity of the BTF, than did inert packing. Thus, the BTF with SMP demonstrated excellent performance, suggesting the suitability of SMP for real applications, whereas the capabilities of inert packing materials are more suited to the treatment of steady low VOC loads. Packing is the most important factor in biofilter design. A structured mixed packing (SMP) material, combined with various organic and inorganic materials (mineral matter is 80.18 ± 0.48%, w : w), was constructed by ureaformaldehyde resin in order to minimize the disadvantages of these materials when used as stand-alone components. The performance of the toluene biotrickling filter (BTF)packed with SMP was compared with the other BTFs packed with a ceramic raschig ring, ceramic pall ring, and lava rock, respectively, for 217 day under various operating conditions. Real-time PCR and DGGE techniques were applied to reveal the gene coding for the toluene-degrading enzymes and the bacterial community structure in the BTFs. The toluene-degradation gene copies exponentially increased, and bacterial diversity significantly decreased with the improving elimination capacities of the BTFs. The overload and shutdown operations resulted in insignificant fluctuations in the toluene-degradation gene copies at equal levels as well as a slight variation in the bacterial community structures in the BTFs. Various putative toluenedegrading bacteria were found using sequencing bands from the DGGE gels; some bacteria, such as Burkholderia spp., were further confirmed by real-time PCR; other bacteria,such as Alcaligenes spp., might not have been reported. The packing properties of SMP material supported more toluene-degradation gene copies in the biofilm, and higher toluene-degrading bacterial diversity of the BTF, than did inert packing. Thus, the BTF with SMP demonstrated excellent performance, suggesting the suitability of SMP for real applications, whereas the capabilities of inert packing materials are more suited to the treatment of steady low VOC loads.

Ciceribacter ferrooxidans sp. nov., a nitrate-reducing Fe(II)-oxidizing bacterium isolated from ferrous ion-rich sediment

Tongchu Deng,Youfen Qian,Xingjuan Chen,Xunan Yang,Jun Guo,Guoping Sun,Meiying Xu 한국미생물학회 2020 The journal of microbiology Vol.58 No.5

A nitrate-reducing Fe(II)-oxidizing bacterial strain, F8825T, was isolated from the Fe(II)-rich sediment of an urban creek in Pearl River Delta, China. The strain was Gram-negative, facultative chemolithotrophic, facultative anaerobic, nonspore- forming, and rod-shaped with a single flagellum. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that it belongs to the genus Ciceribacter and is most closely related to C. lividus MSSRFBL1T (99.4%), followed by C. thiooxidans F43bT (98.8%) and C. azotifigens A.slu09T (98.0%). Fatty acid, polar lipid, respiratory quinone, and DNA G + C content analyses supported its classification in the genus Ciceribacter. Multilocus sequence analysis of concatenated 16S rRNA, atpD, glnII, gyrB, recA, and thrC suggested that the isolate was a novel species. DNA–DNA hybridization and genome sequence comparisons (90.88 and 89.86%, for values of ANIm and ANIb between strains F8825T with MSSRFBL1T, respectively) confirmed that strain F8825T was a novel species, different from C. lividus MSSRFBL1T, C. thiooxidans F43bT, and C. azotifigens A.slu09T. The physiological and biochemical properties of the strain, such as carbon source utilization, nitrate reduction, and ferrous ion oxidation, further supported that this is a novel species. Based on the polyphasic taxonomic results, strain F8825T was identified as a novel species in the genus Ciceribacter, for which the name Ciceribacter ferrooxidans sp. nov. is proposed. The type strain is F8825T (= CCTCC AB 2018196T = KCTC 62948T).

Identification of growth trait related genes in a Yorkshire purebred pig population by genome-wide association studies

Qingli Meng,Kejun Wang,Xiallei Liu,Haishen Zhou,Li Xu,Zhaojun Wang,Meiying Fang 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.4

Objective: The aim of this study is to identify genomic regions or genes controlling growth traits in pigs. Methods: Using a panel of 54,148 single nucleotide polymorphisms (SNPs), we performed a genome-wide Association (GWA) study in 562 pure Yorshire pigs with four growth traits: average daily gain from 30 kg to 100 kg or 115 kg, and days to 100 kg or 115 kg. Fixed and random model Circulating Probability Unification method was used to identify the associations between 54,148 SNPs and these four traits. SNP annotations were performed through the Sus scrofa data set from Ensembl. Bioinformatics analysis, including gene ontology analysis, pathway analysis and network analysis, was used to identify the candidate genes. Results: We detected 6 significant and 12 suggestive SNPs, and identified 9 candidate genes in close proximity to them (suppressor of glucose by autophagy [SOGA1], R-Spondin 2 [RSPO2], mitogen activated protein kinase kinase 6 [MAP2K6], phospholipase C beta 1 [PLCB1], rho GTPASE activating protein 24 [ARHGAP24], cytoplasmic polyadenylation element binding protein 4 [CPEB4], GLI family zinc finger 2 [GLI2], neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adaptor 2 [NYAP2], and zinc finger protein multitype 2 [ZFPM2]). Gene ontology analysis and literature mining indicated that the candidate genes are involved in bone, muscle, fat, and lung development. Pathway analysis revealed that PLCB1 and MAP2K6 participate in the gonadotropin signaling pathway and suggests that these two genes contribute to growth at the onset of puberty. Conclusion: Our results provide new clues for understanding the genetic mechanisms underlying growth traits, and may help improve these traits in future breeding programs.