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Preparation of CdSe QDs-carbohydrate Conjugation and its Application for HepG2 Cells Labeling
Jiang, Mingxing,Chen, Yan,Kai, Guiqing,Wang, Ruijun,Cui, Huali,Hu, Meili Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.2
In present study, CdSe quantum dots (QDs) were prepared with a novel but simple, effective and exercisable method. Nine different types of carbohydrate molecules were used to modify CdSe QDs. D-mannose (Man)-coated quantum dots were prepared for labeling human hepatoma (HepG2) cells, because of the high expression of mannose receptor (MR) on HepG2 cells. The uptake characteristics of CdSe QDs-Man were investigated in HepG2 cells. The absorption rate result of MTT assay in 48 h suggested the extremely low cytotoxicity of CdSe QDs-Man. The presence of quantum dots was confirmed with fluorescence microscopy. These results were encouraging regarding the application of QDs molecules for early detection of HepG2 cells.
Preparation of CdSe QDs-carbohydrate Conjugation and its Application for HepG2 Cells Labeling
Mingxing Jiang,Yan Chen,Guiqing Kai,Ruijun Wang,Huali Cui,Meili Hu 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.2
In present study, CdSe quantum dots (QDs) were prepared with a novel but simple, effective and exercisable method. Nine different types of carbohydrate molecules were used to modify CdSe QDs. D-mannose (Man)-coated quantum dots were prepared for labeling human hepatoma (HepG2) cells, because of the high expression of mannose receptor (MR) on HepG2 cells. The uptake characteristics of CdSe QDs-Man were investigated in HepG2 cells. The absorption rate result of MTT assay in 48 h suggested the extremely low cytotoxicity of CdSe QDs-Man. The presence of quantum dots was confirmed with fluorescence microscopy. These results were encouraging regarding the application of QDs molecules for early detection of HepG2 cells.