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RISS 인기검색어
- 검색키워드
- 저자 : Mehmet S¸ enel (검색결과 3 건)
- 무료
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Mehmet S¸ enel,Muamer Dervisevic,Emre Çevik 한국물리학회 2013 Current Applied Physics Vol.13 No.7
The biosensor was constructed for determination of glucose by using glucose oxidase enzyme immobilized on poly(thiophene-3-boronic acid) (PTBA). Boronic acid functionalized polythiophene layer was obtained by electrochemical polymerization of Thiophene (Th) and thiophene-3-boronic acid (TBA) with different monomer rations. The reconstitution of the apo-glucose oxidase (apo-GOx) on a complexed flavin adenine dinucleotide (FAD) linked to polythiophene boronic acid (PTBA) monolayer yields an electrically contacted enzyme monolayer. The GOx-reconstituted enzyme electrode exhibited excellent electrocatalytic activities toward the reduction and oxidation of hydrogen peroxide as well. The PTBA/FAD/GOx biosensor shows an excellent performance for glucose at þ0.4 V with a high sensitivity (2.14 mA/mM) and lower response time (w5 s) in a wide concentration range of 0.5e18 mM (correlation coefficient of 0.9952). Furthermore, the effects of applied potential, pH, temperature, electroactive interference, stability and reusability of the biosensors were discussed.
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Emre Çevik,Mehmet S¸ enel,Abdulhadi Baykal,M. Fatih Abasıyanık 한국물리학회 2013 Current Applied Physics Vol.13 No.8
An amperometric phenol biosensor was constructed by using poly(glycidylmethacrylate-co-vinyl ferrocene) grafted iron oxide nanoparticles for detection of different phenolic compounds (catechol, aminophenol, phenol, p-cresol, pyrogallol). The poly(glycidylmethacrylate-co-vinyl ferrocene) and nanoparticles were characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD) and transmission electron microscopy (TEM). The copolymer grafted iron oxide nanoparticles and Horseradish peroxidase (HRP) were covalently attached on gold (Au) electrode surface. The effect of pH, temperature and characteristic features such as; reusability and storage stability were studied. The electrode showed good response time within w3 s. The electrocatalytic response showed a linear dependence on the phenolic compounds concentration ranging from 0.5 to 17.0 mM.
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Potentiometric urea biosensor based on poly(glycidylmethacrylate)-grafted iron oxide nanoparticles
Emre Çevik,Mehmet S¸ enel,Abdülhadi Baykal 한국물리학회 2013 Current Applied Physics Vol.13 No.1
Urease enzyme was covalently attached on the poly(glycidylmethacrylate) (PGMA)-grafted iron oxide nanoparticles on Au electrode for the fabrication of urea biosensor. The telomere of poly(- glycidylmethacrylate) (PGMA) with a trimethoxysilyl terminal group was synthesized by telomerization of glycidylmethacrylate. Iron oxide nanoparticles were coated with telomere of poly(- glycidylmethacrylate) in order to obtain good enzyme immobilization platform. The telomere and nanoparticles were characterized by using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and thermal gravimetric analysis (TGA). The biosensor’s potentiometric response was measured as a function of urea concentration in phosphate buffer solution (10 mM, pH 7.5) and showed a linear range of 0.25e5.0 mM urea. The produced biosensor exhibited a good response time of w8 s and was stable for about two months. The basic features (optimum pH, optimum temperature, interference and storage stability) of the enzyme electrode were determined
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