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RISS 인기검색어
- 검색키워드
- 저자 : Mehede Hassan Rubel (검색결과 4 건)
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Development of Molecular Markers for Specific Detection of Xanthomonas campestris pv. incanae
( Mehede Hassan Rubel ),( Denison Michael Immanuel Jesse ),( Ujjal Kumar Nath ),( Jung-hee Jeong ),( Hoy-taek Kim ),( Jong-in Park ),( Ill-sup Nou ) 한국육종학회 2021 Plant Breeding and Biotechnology Vol.9 No.4
Xanthomonas campestris pv. incanae (Xci) is the causal agent of bacterial blight disease in ornamental crucifers. We compared the whole genomes of closely related Xanthomonas campestris pv. campestris, incanae, raphani and four other species of Xanthomonas following comparative genomics approach. We found 82 singletons out of 4024 Xci genes upon comparison. Out of 82 singletons, top 10 were selected for designing Xci specific marker. Five primers; XCI_1F/R, XCI_2F/R, XCI_3F/R, XCI_5F/R and XCI_6F/R produced amplicons of 495 bp, 503 bp, 612 bp, 665 bp and 468 bp, respectively expected to detect Xanthomonas campestris pv. incanae (Xci). In conclusion, five effective markers were developed for the detection of Xci pathogen by whole genome alignment, which could be served as effective tools in seed quarantine.
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Mehede Hassan Rubel,Sathishkumar Natarajan,Ujjal Kumar Nath,Michael Immanuel Jesse Denison,정희정,Hoy-Taek Kim,Jong-In Park,Ill-Sup Nou 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.4
Xanthomonas campestris pv. campestris ( Xcc ) is a plant pathogen of cruciferous crops that causes black rot disease throughout the world. At present, based on the host–pathogen interactions among diff erential cultivars of Brassica crops, 11 pathogenic Xcc races have been identifi ed, but the race identifi cation method based on host–pathogen interactions is time-consuming. However, early and rapid detection of the pathogen could reduce economic loss by allowing appropriate control measures against black rot disease to be taken more quickly. In this study, a PCR-based molecular marker has been developed for identifying the Xcc race 1 and Xcc race 2 bacterial strains together. The specifi city of the marker was tested by PCR using 8 available Xcc races, X. campestris strains, and other bacteria. Upon amplifi cation, a polymorphic band was observed in the PCR amplicon with a size of 1523 bp and 929 bp in Xcc races 1 and 2, respectively. A deletion of 594 bp conferred the specifi city in Xcc race 2 compared to race 1. The identifi ed PCR-based molecular marker clearly discriminated the Xcc race 1 and race 2 from other races when tested in artifi cially infected cabbage leaves. Thus, PCR-based development of an Xcc race 1- and 2-specifi c marker could be a valuable tool for the accurate detection of Xcc race 1 and 2 together for implementing control measures more quickly.
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Khandker Shazia Afrin,Md Abdur Rahim,Mehede Hassan Rubel,Jong-In Park,Hee-Jeong Jung,Hoy-Taek Kim,Ill-Sup Nou 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.5
Xanthomonas campestris pv. campestris (Xcc), the pathogen of black rot which is the most destructive disease of Brassica vegetables throughout the world. Here, we reported two novel sequence-characterized amplified region (SCAR) markers (i.e., XccR6-60 and XccR6-67) for the detection of Xcc race 6 via re-alignment of the complete genome sequences of Xcc races/ strains/pathovars. The specificity of SCAR primer sets was verified by mean of PCR amplification using the genomic DNA template of Xcc races/strains/pathovars and two other plant infecting bacterial strains. The PCR result revealed that the XccR6-60 and XccR6-67 primer sets amplified 692-bp and 917-bp DNA fragments, respectively, specifically from race 6, while no visible amplification was detected in other samples. In addition, the SCAR primers were highly sensitive and can detect from a very low concentration of genomic DNA of Xcc race 6. However, the complete genome sequence of Xcc race 6 is not yet publicly available. Therefore, the cloning and sequencing of XccR6-60 and XccR6-67 fragments from race 6 provide more evidence of the specificity of these markers. These results indicated that the newly developed SCAR markers can successfully, effectively and rapidly detect Xcc race 6 from other Xcc races/strains/pathovars as well as other plant pathogenic bacteria. This is the first report for race-specific molecular markers for Xcc race 6.
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Screening of Cabbage (Brassica oleracea L.) Germplasm for Resistance to Black Rot
Khandker Shazia Afrin,Md Abdur Rahim,박종인,Sathishkumar Natarajan,Mehede Hassan Rubel,김회택,노일섭 한국육종학회 2018 Plant Breeding and Biotechnology Vol.6 No.1
Black rot of Brassica crops is the most devastating disease which causes substantial yield reduction of cabbagethroughout the world. The use of resistant cabbage cultivars could be inexpensive and effective measure to combat this destructivedisease. We screened cabbage inbred lines for black rot disease resistance through bioassay and identified some novel lines that showedrace-specific resistance to Xanthomonas campestris pv. campestris (Xcc) races. The pathogenicity test revealed that out of 27 cabbagelines, one (SCNU-C-4074), six (SCNU-C-3631, SCNU-C-3637, SCNU-C-3639, SCNU-C-4072, SCNU-C-4073 and SCNU-C-3273),two (SCNU-C-3273 and SCNU-C-4118), two (SCNU-C-3270 and SCNU-C-4118), two (SCNU-C-3470 and SCNU-C-41148) and four(SCNU-C-107, SCNU-C-3270, SCNU-C-3470 and SCNU-C-4059) were shown to be resistant to Xcc races 1, 2, 3, 5, 6 and 7,respectively while none of these showed resistance against race 4. Furthermore, these resistant and susceptible lines were evaluated bypreviously reported molecular markers for black rot resistance. The molecular screening results were also revealed the existence ofrace-specific resistance in these cabbage lines. This result will help Brassica breeder to develop race-specific black rot resistant cabbagecultivars.
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