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        Genomics of brown algae: current advances and future prospects

        J. Mark Cock,Alok Arun,Olivier Godfroy,Nicolas Macaisne,Martina Strittmatter,Akira F. Peters,Susana M. Coelho 한국유전학회 2012 Genes & Genomics Vol.34 No.1

        The analysis of the complete genome sequence of the filamentous brown alga Ectocarpus provided many new insights into brown algal biology and has improved our understanding of how this organism has adapted to its seashore environment. Since the publication of the genome sequence in 2010, numerous studies have continued the analysis of the constituent genes or have combined genome data with experimental work,allowing progress in several key areas, including metabolism and reproductive biology. Ectocarpus will continue to be exploited as a model organism in future years, but genomic approaches should also be extended to additional brown algal species in order to fully exploit the diversity of this phylogenetic group and to facilitate the application of new knowledge to economically important seaweeds such as kelps.

      • The Algal Revolution

        Brodie, Juliet,Chan, Cheong Xin,De Clerck, Olivier,Cock, J. Mark,Coelho, Susana M.,Gachon, Claire,Grossman, Arthur R.,Mock, Thomas,Raven, John A.,Smith, Alison G.,Yoon, Hwan Su,Bhattacharya, Debashish Elsevier 2017 Trends in plant science Vol.22 No.8

        <P>Algae are (mostly) photosynthetic eukaryotes that occupy multiple branches of the tree of life, and are vital for planet function and health. In this review, we highlight a transformative period in studies of the evolution and functioning of this extraordinary group of organisms and their potential for novel applications, wrought by high-throughput ‘omic’ and reverse genetic methods. We cover the origin and diversification of algal groups, explore advances in understanding the link between phenotype and genotype, consider algal sex determination, and review progress in understanding the roots of algal multicellularity. Experimental evolution studies to determine how algae evolve in changing environments are highlighted, as is their potential as production platforms for compounds of commercial interest, such as biofuel precursors, nutraceuticals, or therapeutics.</P> <P><B>Trends</B></P> <P>Application of modern ‘omic and genetic methods has significantly advanced our understanding of the origin, evolution, and metabolic potential of unicellular and multicellular algae, as well as their diverse modes of sexual reproduction.</P> <P>The GreenCut proteins, a conserved gene set in the Viridiplantae, are primarily plastid targeted and have key roles in the function and regulation of photosynthesis, including the maintenance of photosynthetic reaction complexes.</P> <P>Lab evolution experiments demonstrate the strong adaptability of microalgae to environmental changes that are associated with climate change, although it is unclear whether these results will hold in natural ecosystems.</P> <P>The development of algae as ‘cell factories’ promises to allow the production of not only endogenous molecules, but also non-native compounds such as high-value pigments, bulk chemicals, or even therapeutic proteins.</P>

      • A sequence‐tagged genetic map for the brown alga <i>Ectocarpus siliculosus</i> provides large‐scale assembly of the genome sequence

        Heesch, Svenja,Cho, Ga Youn,Peters, Akira F.,Le Corguillé,, Gildas,Falentin, Cyril,Boutet, Gilles,Coë,del, Solè,ne,Jubin, Claire,Samson, Gaelle,Corre, Erwan,Coelho, Susana M.,Mark Cock Blackwell Publishing Ltd 2010 The New phytologist Vol.188 No.1

        <P><B>Summary</B></P><P><P> <I>Ectocarpus siliculosus</I> has been proposed as a genetic and genomic model for the brown algae and the 214 Mbp genome of this organism has been sequenced. The aim of this project was to obtain a chromosome‐scale view of the genome by constructing a genetic map using microsatellite markers that were designed based on the sequence supercontigs.</P><P>To map genetic markers, a segregating F<SUB>2</SUB> population was generated from a cross between the sequenced strain (Ec 32) and a compatible strain from northern Chile. Amplified fragment length polymorphism (AFLP) analysis indicated a significant degree of polymorphism (41%) between the genomes of these two parental strains. Of 1,152 microsatellite markers that were selected for analysis based on their location on long supercontigs, their potential as markers and their predicted ability to amplify a single genomic locus, 407 were found to be polymorphic.</P><P>A genetic map was constructed using 406 markers, resulting in 34 linkage groups. The 406 markers anchor 325 of the longest supercontigs on to the map, representing 70.1% of the genome sequence.</P><P>The <I>Ectocarpus</I> genetic map described here not only provides a large‐scale assembly of the genome sequence, but also represents an important tool for future genetic analysis using this organism.</P></P>

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