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RISS 인기검색어
- 검색키워드
- 저자 : Akira F. Peters (검색결과 3 건)
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Heesch, Svenja,Cho, Ga Youn,Peters, Akira F.,Le Corguillé,, Gildas,Falentin, Cyril,Boutet, Gilles,Coë,del, Solè,ne,Jubin, Claire,Samson, Gaelle,Corre, Erwan,Coelho, Susana M.,Mark Cock Blackwell Publishing Ltd 2010 The New phytologist Vol.188 No.1
<P><B>Summary</B></P><P><P> <I>Ectocarpus siliculosus</I> has been proposed as a genetic and genomic model for the brown algae and the 214 Mbp genome of this organism has been sequenced. The aim of this project was to obtain a chromosome‐scale view of the genome by constructing a genetic map using microsatellite markers that were designed based on the sequence supercontigs.</P><P>To map genetic markers, a segregating F<SUB>2</SUB> population was generated from a cross between the sequenced strain (Ec 32) and a compatible strain from northern Chile. Amplified fragment length polymorphism (AFLP) analysis indicated a significant degree of polymorphism (41%) between the genomes of these two parental strains. Of 1,152 microsatellite markers that were selected for analysis based on their location on long supercontigs, their potential as markers and their predicted ability to amplify a single genomic locus, 407 were found to be polymorphic.</P><P>A genetic map was constructed using 406 markers, resulting in 34 linkage groups. The 406 markers anchor 325 of the longest supercontigs on to the map, representing 70.1% of the genome sequence.</P><P>The <I>Ectocarpus</I> genetic map described here not only provides a large‐scale assembly of the genome sequence, but also represents an important tool for future genetic analysis using this organism.</P></P>
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Assessment of the macroalgal diversity of Kuwait by using the Germling Emergence Method
Amal H. Hajiya Hasan,Dhia A. Al-Bader,Steve Woodward,Csongor Z. Antony,Jared Kok Ong,Akira F. Peters,Frithjof C. Kupper The Korean Society of Phycology 2023 ALGAE Vol.38 No.2
Cryptic stages of diverse macroalgae present in natural substrata, "the bank of microscopic forms", were isolated into clonal cultures and identified based on both morphological characteristics and DNA barcoding. Approximately 120 clonal isolates from 308 natural substratum samples were collected from the entire coastline of Kuwait. Amongst these isolates, 77 (64%) were identified through DNA barcoding using the nuclear ribosomal small subunit, RuBisCO spacer (ITS2, tufa, rbcL, psaA, and psbA) and sequencing. Twenty-six isolates (34%) were identified in the division Chlorophyta, 18 (23%) as Phaeophyceae, and 33 (43%) as Rhodophyta. For all DNA sequences in this study, species-level cut off applied was ≥98% homology which depend entirely on the markers used. Three putative new records of Chlorophyta new for the Arabian Gulf were made: Cladophora laetevirens (Dillwyn) Kützing, Ulva torta (Mertens) Trevisan and Ulvella leptochaete (Huber) R. Nielsen, C. J. O'Kelly & B. Wysor in Nielsen, while Cladophora gracilis Kützing and Ulva ohnoi M. Hiraoka & S. Shimada are new records for Kuwait. For Phaeophyceae, Ectocarpus subulatus Kützing and Elachista stellaris Areschoug were new records for the Gulf and Kuwait. In the Rhodophyta, Acrochaetium secundatum (Lyngbye) Nägeli in Nägeli & Cramer, Ceramium affine Setchell & N. L. Gardner, Gelidium pusillum var. pakistanicum Afaq-Husain & Shameel and Dasya caraibica Børgesen are new records for the Gulf and Kuwait, while the red alga Stylonema alsidii (Zanardini) K. Drew is a new record for Kuwait. Several isolates identified corresponded to genera not previously reported in Kuwait and / or the Arabian Gulf, such as Porphyrostromium Trevisan, a new genus from the Bangiales, and two unidentified species for the Planophilaceae Škaloud & Leliaert. The isolates cultivated from substrata enhance understanding of the marine macroalgal diversity in the region and confirmed that the Germling Emergence Method is suitable for determining the actual diversity of a given study area through isolation from cryptic life-history phases.
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Genomics of brown algae: current advances and future prospects
J. Mark Cock,Alok Arun,Olivier Godfroy,Nicolas Macaisne,Martina Strittmatter,Akira F. Peters,Susana M. Coelho 한국유전학회 2012 Genes & Genomics Vol.34 No.1
The analysis of the complete genome sequence of the filamentous brown alga Ectocarpus provided many new insights into brown algal biology and has improved our understanding of how this organism has adapted to its seashore environment. Since the publication of the genome sequence in 2010, numerous studies have continued the analysis of the constituent genes or have combined genome data with experimental work,allowing progress in several key areas, including metabolism and reproductive biology. Ectocarpus will continue to be exploited as a model organism in future years, but genomic approaches should also be extended to additional brown algal species in order to fully exploit the diversity of this phylogenetic group and to facilitate the application of new knowledge to economically important seaweeds such as kelps.
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