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      저자 : Mark Christopher Jenkins (검색결과 2 건)
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      • Avian leucocyte common antigens : molecular weight determination and flow cytometric analysis using new monoclonal antibodies

        Chung, Kyeong-Soo,Lillehoj, Hyung Soon,Mark, Christopher Jenkins 충남대학교 약학대학 의약품개발연구소 1991 藥學論文集 Vol.7 No.-

        Chung, K.S., Lillehoj, H.S. and Jenkins, M.C., 1991. Avian leucocyte common antigens: molecular weight determination and flow cytometric analysis using new monoclonal antibodies. Vet. Immunol. Immunopathol., 28:259-273. New leucocyte common antigens expressed on all the normal chicken leucocytes have been characterized using two monoclonal antibodies designated as K-11 and K-55. These monoclonal antibodies stained virtually 100% of the leucocytes derived from various lymphoid organs including the spleen, thymus, bursa of Fabricius, caecal tonsil and peripheral blood, as well as a monocytic cell line (MC29). a B cell line (LSCC-RP9), and a T cell line (CU12). However, they did not stain mature erythrocytes, intestinal epithelial cells, or chicken embryonic fibroblasts. The two monoclonal antibodies showed different staining patterns and detected non-overlapping epitopes on MC29 cells in two color immunofluorescence analysis. Western blot analysis under non-reducing conditions showed that the monoclonal antibody K-11 recongnized three splenic leucocyte proteins with molecular weights of 92,42 and 41 kDa, whereas the monoclonal antibody K-55 recognized two proteins with molecular weights of 97 and 42 kDa. The data indicate that the monoclonal antibodies K-11 and K-55 recognize novel leucocyte-common antigens which have lower molecular weights than the previously reported leucocyte-common antigen family.

      • Avian leucocyte common antigens : molecular weight determination and flow cytometric analysis using new monoclonal antibodies

        Chung, Kyeong-Soo,Hyun Soon Lillehoj,Mark Christopher Jenkins 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

        Chung, K.S., Lillehoj, H.S. and Jenkins, M.C., 1991. Avian leucocyte common antigens: molecular weight determination and flow cytometric analysis using new monoclonal antibodies. Vet. Immunol. Immunopathol., 28: 259-273. New leucocyte common antigens expressed on all the normal chicken leucocytes have been characterized using two monoclonal antibodies designated as K-11 and K-55. These monoclonal antibodies stained virtually 100% of the leucocytes derived from various lymphoid organs including the spleen, thymus, bursa of Fabricius, caecal tonsil and peripheral blood, as well as a monocytic cell line (MC29), aB cell line (LSCC-RP9), and a T cell line (CU12). However, they did not stain mature erythrocytes, intestinal epithelial cells, or chicken embryonic fibroblasts. The two monoclonal antibodies showed different staining patterns and detected non-overlapping epitopes on MC29 cells in two color immunofluorescence analysis. Western blot analysis under non-reducing conditions showed that the monoclonal antibody K-11 recognized three splenic leucocyte proteins with molecular weights of 92, 42 and 41 kDa, whereas the monoclonal antibody K-55 recognized two proteins with molecular weights of 97 and 42 kDa. The data indicate that the monoclonal antibodies K-11 and K-55 recognize novel leucocyte-common antigens which have lower molecular weights than the previously reported leucocyte-common antigen family.

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