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Duana Ceciliane Hanauer,Alana Gabrieli de Souza,Mariana Aguiar Cargnin,Bruna Carla Gasparin,Derval dos Santos Rosa,Alexandre Tadeu Paulino 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.97 No.-
In this work, pectin-based biohydrogels with and without eucalyptus sawdust were synthesized viaradical crosslinking and characterized by Fourier transform infrared (FT-IR) spectroscopy, scanningelectron microscopy (SEM), swelling assays (SA), thermogravimetric analysis (TGA), differential scanningcalorimetry (DSC), X-ray diffraction (XRD), and rheological tests (RT). The immobilization of β-Dgalactosidasein the biohydrogel networks was performed by adsorption, and the immobilized enzymeactivity was studied during lactose hydrolysis reactions. Strong inter and intramolecular interactionsamong enzyme and biohydrogel chemical groups increased the biopolymer network crosslinkingdensities, decreasing the swelling capacities. The water diffusion mechanism in the biopolymer networkswas governed by non-Fickian transport, being affected by the pH and temperature values of the aqueoussolutions. The incorporation of eucalyptus sawdust in the biopolymer network increased thefinalbiomaterial thermal and mechanical resistance, preserving the immobilized enzyme stability. Pectinbasedbiohydrogels reinforced with eucalyptus sawdust proved to be potential solid supports for theimmobilization of β-D-galactosidase. These polymeric matrices preserved the enzyme stability in moreacidic aqueous solutions and enabled the application of the immobilized enzyme for at least sixsuccessive lactose hydrolysis cycles. Overall, β-D-galactosidase immobilized in pectin-based biohy-drogels appears as an alternative for the production of either lactose-free or low-dosage lactose foods.