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        Development of mycorrhized vitroplants of Jatropha curcas L. at different rooting stages

        Muriel da Silva Folli-Pereira,Maria Catarina Megumi Kasuya,Lydice Sant’Anna Meira-Haddad,Nazima Rasool,Wagner Campos Otoni 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.4

        In this work, we attempted to assess the effects of inoculation of arbuscular mycorrhizal fungus (AMF),Glomus clarum, on the survival and development of micropropagated Jatropha curcas plantlets at different rooting stages. Elongated shoots (average 3 cm) of J. curcas,maintained for 0, 14, or 21 days on rooting medium in the presence or absence of 1 mg L-1 indole-3-butyric acid (IBA), were transferred to a sand:soil:vermiculite (1:1/2:1)(v:v:v) substrate. At the time of transplantation, the plantlets were either inoculated or not inoculated with G. clarum that had been monoaxenically produced in Ri-transformed carrot roots. After a 2-week acclimatization period, 100 %of the plants kept for 0 or 14 days in rooting medium survived. However, those that remained for 21 days in rooting medium displayed post-acclimatization survival rates of 93 and 89 % for plants inoculated and non-inoculated with G. clarum, respectively. Colonization rates ranged from 70 to 93 %, and the stimulatory effects of AMF were evidenced by increased phosphorus uptake by plants and increases in all evaluated growth parameters,except plant height. Plants that were not subjected to the rooting stage showed growth similar to or higher than those subjected to the rooting stage, regardless of the addition of IBA. It can be concluded that stimulatory effects of mycorrhizal fungi were observed, and that the inoculation of J. curcas proved effective during the initial period of the acclimatization phase.

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        Food Value and Safety Status of Raw (Unfermented) and Fermented Higher Basidiomycetes, Lenzites quercina (L) P. Karsten

        Clement Olusola Ogidi,Victor Olusegun Oyetayo,Bamidele Juliet Akinyele,Camilo Amaro De Carvalho,Maria Catarina Megumi Kasuya 한국식품영양과학회 2018 Preventive Nutrition and Food Science Vol.23 No.3

        Food value and safety of a wild macrofungus, Lenzites quercina were investigated. The proximate and mineral composition of raw and fermented L. quercina were assessed using standard methods. Brine shrimp lethality bioassay was adopted for assessment of the toxicological property of the extracts obtained from raw and fermented L. quercina. The result of proximate composition revealed higher protein content (26.15%) in fermented L. quercina, while the value of carbohydrate (38.3%), crude fibre (27.6%), and ash contents (6.5%) were higher (P<0.05) in raw L. quercina when compared to fermented L. quercina. The macro and micro elements in the raw and fermented L. quercina were in decreasing order of Ca> K> Zn> Fe> Na> Mg> Pb> Cu with values ranging from 4.04 mg/g to 721.6 mg/g. The amino acids in raw and fermented L. quercina ranged from 0.05 mg/g to 23.78 mg/g, while the fatty acids ranged from 0.11% to 38.5%. The mortality rate of the Artemia salina against the extracts was from 8.0% to 38.0% with lethal dose at 50% of population within 49.11 and 250.50 g/mL. The results from this study revealed that L. quercina possesses essential amino acids, fatty acids, and substantial micro elements, which may be useful in the formulation of functional foods and nutraceuticals.

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