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Manian, S,Paulsamy, S.,Senthilkumar, K.,Kil, Bong-Seop The Ecological Society of Korea 2002 Journal of Ecology and Environment Vol.25 No.4
Dynamics of certain $N_2$ fixing bacteria such as Rhizobium, Azospirillum and Azotobactor, nodule number in dominant legume, Atylosia trinervia, P-solubilizing bacteria, actinomycetes and fungi were studied in unburned and burned site of natural grassland, southern India. Population of $N_2$ - fixing bacteria, P-solubilizing bacteria, fungi and nodule number in legume increased significantly in burned sites. On the other hand, the actino-mycetes population remained unchanged. Thirty six species of fungi with tricalcium phosphate solubilizing ability were recorded. The most efficient P-solubilizing fungi recognised in the soils of the study sites are Absidia ramosa, Gongronella butlerii, Mortieralla spinosa, Mucor racemosus, Rhizopus nigricans, R. stolonifer, R. oryzae, Aspergillus fumigatus, A. nidulans, A. niger, Theilavia terricola and Cheatomium lunasporium.
Kumar, Manian Rajesh,Park, Ahbyeol,Park, Namjin,Lee, Sunwoo American Chemical Society 2011 ORGANIC LETTERS Vol.13 No.13
<P>2<I>H</I>-Indazoles are synthesized using copper-catalyzed, one-pot, three-component reactions of 2-bromobenzaldehydes, primary amines, and sodium azide. A copper catalyst plays the key role in the formation of C–N and N–N bonds. This method has a broad substrate scope with a high tolerance for a variety of functional groups.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/orlef7/2011/orlef7.2011.13.issue-13/ol201409j/production/images/medium/ol-2011-01409j_0001.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ol201409j'>ACS Electronic Supporting Info</A></P>
Silica-Polyaniline Based Bienzyme Cholesterol Biosensor: Fabrication and Characterization
Manesh, Kalayil Manian,Santhosh, Padmanabhan,Gopalan, Anantha Iyengar,Lee, Kwang-Pill WILEY-VCH Verlag 2010 Electroanalysis Vol.22 No.20
<P>In the present investigation, silica-polyaniline based bienzyme cholesterol biosensor is fabricated through a simple one-step electrochemical method. The one-step fabrication process involves electrochemical polymerization of N[3-(trimethoxysilyl)propyl]aniline to result poly(N[3-(trimethoxysilyl)propyl]aniline) (PTMSPA) and simultaneous immobilization of two enzymes, horseradish peroxidase (HRP) and cholesterol oxidase (ChOx) into PTMSPA matrix. The modified electrode is designated as PTMSPA-HRP/ChOx-ME. PTMSPA facilitates direct electron transfer between the electrode surface and the active redox centers of HRP. This enables the operation of a biosensor at a low working potential of about −150 mV (vs. Ag/AgCl) for the detection of hydrogen peroxide. The PTMSPA-HRP/ChOx-ME demonstrates excellent analytical performance for the detection of cholesterol between 1 and 25 mM with high sensitivity and selectivity. PTMSPA possesses features suited for the fabrication of third-generation biosensors.</P>
Santhosh, Padmanabhan,Manesh, Kalayil Manian,Lee, Se-Hee,Uthayakumar, Sivaperumal,Gopalan, Anantha Iyengar,Lee, Kwang-Pill Royal Society of Chemistry 2011 The Analyst Vol.136 No.8
<P>In the present communication, a novel composite nanofibrous electrode is developed for the detection of superoxide anion (O<SUB>2</SUB>˙<SUP>−</SUP>) in phosphate buffered saline (PBS). The composite fiber electrode is fabricated by dispersing gold nanoparticles onto poly(methyl methacrylate) (PMMA)–polyaniline (PANI) core–shell electrospun nanofibers. The constructed architecture is proven to be a favorable environment for the immobilization of the enzyme, superoxide dismutase (SOD). Direct electron transfer is achieved between SOD and the electrode with an electron transfer rate constant of 8.93 s<SUP>−1</SUP>. At an applied potential of +300 mV, PMMA/PANI–Au<SUB>nano</SUB>/SOD–ESCFM shows highly sensitive detection of O<SUB>2</SUB>˙<SUP>−</SUP>. In addition to this, quantification of different activities of SOD is realized at PMMA/PANI–Au<SUB>nano</SUB>/SOD–ESCFM. These analytical features offer great potential for construction of the third-generation O<SUB>2</SUB>˙<SUP>−</SUP> biosensor.</P> <P>Graphic Abstract</P><P>A novel composite nanofibrous electrode is developed for the detection of superoxide anion in phosphate buffer saline. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0an00616e'> </P>
Mitochondria-specific conjugated polymer nanoparticles
Twomey, Megan,Mendez, Eladio,Manian, Rajesh Kumar,Lee, Sunwoo,Moon, Joong Ho The Royal Society of Chemistry 2016 Chemical communications Vol.52 No.27
<P>Biodegradable conjugated polymer nanoparticles (CPNs) were prepared for high mitochondrial targeting in live cancer cells. The degradable CPNs are nontoxic and specifically localized to the mitochondria of live tumor cells through macropinocytosis followed by intracellular degradation and trafficking.</P>
Fire and Rangelands Management in India
Paulsamy, S.,S. Manian,길봉섭 한국생태학회 2005 Journal of Ecology and Environment Vol.28 No.1
Fire, usualy a detrimental factor makes changes in comonly structure and functions when it ispracticed properly. In countries like Australia, South Africa, Japan and Texas in USA etc., fire is used as a tol to manage the natural ecosystems in productive condition on the basis of ecological approach. However, in developing countries like India there is efective measure to use and control fire in rangelands. This review provides knowledge on changes in sociological characters of plants, biomass profile, cycling of materials and Further, the informations furnished in this article will be more useful for the forest managers, planers of rangelands and administrators in countries like India and tropical countries with similar bioclimatic conditions to prepare appropriate management plans where the fire is an integral factor.
Kim, Yong,Kumar, Manian Rajesh,Park, Namjin,Heo, Yumi,Lee, Sunwoo American ChemicalSociety 2011 Journal of organic chemistry Vol.76 No.23
<P>Benzimidazoles were synthesized by the copper-catalyzed,one-pot,three-component reaction of 2-haloanilines, aldehydes, and NaN<SUB>3</SUB>. The reaction was optimized when 2-iodo- or 2-bromoanilines(1.0 equiv), aldehydes (1.2 equiv), NaN<SUB>3</SUB> (2.0 equiv), 5mol% of CuCl, and 5 mol % of TMEDA were reacted in DMSO at 120 °Cfor 12 h. Good yields resulted, and the reaction showed tolerancetoward functional groups such as ester, nitro, and chloro. Aliphaticand heteroaromatic aldehydes also afforded the desired products inmoderate to good yields.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/joceah/2011/joceah.2011.76.issue-23/jo2019416/production/images/medium/jo-2011-019416_0011.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jo2019416'>ACS Electronic Supporting Info</A></P>
In vitro Antioxidant Properties of Indigenous Underutilized Fruits
Nataraj Loganayaki,Sellamuthu Manian 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.3
In the present study, in vitro antioxidant and free radical scavenging capacity of methanol extracts from 10 underutilized fruits viz., Syzygium cumini, Murraya koenigii, Coccinia grandis, Opuntia dillenii, Carissa carandus, Kirganalia reticulata, Canthium parviflorum,Lantana camara, Alangium lamarckii, and Morus alba were evaluated using established in vitro models such as ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picryl-hydrazyl (DPPH•), 2,2'azinobis(3-ethylbenzothiozoline-6-sulfonic acid) diammonium salt (ABTS), hydroxyl radical (OH•), nitric oxide radical (NO•), super oxide radical (O2• −) scavenging, and metal chelating activities. All the fruit extracts contained substantial concentration of total phenolics, tannins, and flavonoids. The extracts of O. dillenii, M. koenigii, K. reticulata, L. camara, and M. alba registered higher activity in DPPH•, ABTS• +, and FRAP assays. Phenolic content of these fruits is significantly correlated with antioxidant capacity. Interestingly, all the extracts showed considerable nitric oxide, super oxide, and hydroxyl radical scavenging activities in a dose dependant manner when compared with the standard butylatedhydroxyl anisole (BHA). Our findings revealed that these underutilized fruits have potential as good sources of natural antioxidant/nutraceutical compounds.