http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Mahdieh BIGHAM (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
Mahdieh Bigham,Vahid Hosseininaveh 한국응용곤충학회 2010 Journal of Asia-Pacific Entomology Vol.13 No.3
Proteolytic activity in the digestive system of the pistachio green stink bug, Brachynema germari, was investigated. The maximum total proteolytic activity in the midgut extract was observed at pH 5, suggesting the presence of cysteine proteases. Hydrolyzing the specific substrates for cysteine proteases revealed the presence of cathepsin B and cathepsin L activities in the midgut extract. The presence of cysteine proteases was confirmed by their noticeable inhibition and activation due to specific inhibitors and activators,respectively. The significant inhibition of chymotryptic activity by the inhibitors showed the presence of chymotrypsin in the midgut. No considerable tryptic activity was observed in the midgut extract. There was no detectable total proteolytic activity in the salivary gland extract. Tryptic activity of the salivary gland extract was also inhibited by the specific inhibitors. The substrates for cysteine proteases were also slightly hydrolyzed by the salivary gland extract. Zymogram analysis showed at least one distinct band due to cysteine protease activity in the midgut extract, and the cysteine protease inhibitor caused almost complete disappearance of the band. Cathepsin B and L activities were mainly detected in midgut divisions m1 and m3,respectively, and maximum chymotrypsin and trypsin activities were observed in m3. In general, the results revealed the significant presence of cathepsin B, cathepsin L, and chymotrypsin proteases in the midgut extract. The major proteolytic activity in the salivary glands seems to be conducted by trypsin-like proteases.
-
Effects of essential oil from Teucrium polium on some digestive enzyme activities of Musca domestica
Mahdieh BIGHAM,Vahid HOSSEININAVEH,Banafsheh NABAVI,Khalil TALEBI,Naimeh-Sadat ESMAEILZADEH 한국곤충학회 2010 Entomological Research Vol.40 No.1
The essential oil from Teucrium polium was evaluated for its adverse effects on larval instars of Musca domestica. The essential oil was blended with a diet at a concentration that produced 50% mortality of subjected insects (LC50) (80 ppm). To learn about the situation of digestive enzymes of M. domestica treated with the essential oil, third larval instars were dissected under non-active enzyme conditions and their midguts were removed. The supernatant was used as an enzyme source after homogenization and centrifugation of the homogenates. Results revealed that some main digestive enzymes in the larval midgut were adversely affected when exposed to the food-incorporated essential oil. Proteinase extracted from larval midgut hydrolyzed the synthetic substrates B-Arg-pNA, Z-Arg-Arg-PNA and Z-Phe-Arg-PNA for trypsin, cathepsin B and cathepsin L activities, respectively, in control and treated larvae. The essential oil caused a reduction of 61.5% in tryptic activity. Significant 69% and 79% reductions were also observed in cathepsin L and B activities, respectively. Carbohydrase activities of α-amylase, α-glucosidase and β-glucosidase were detected in larval midgut extract. All assayed carbohydrases were affected by the essential oil. The most notable impact, a 93% reduction, was observed in α-amylase. Decreases of 69.5% and 42% were obtained in α-glucosidase and β-glucosidase activity, respectively. This study indicated that the larvicidal effect of the essential oil from Teucrium polium may be due to its detrimental effects on digestive enzymes. It seems that the detrimental effect of the oil can be due to both the inhibitory nature of the oil and the destruction of the midgut epithelium.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
