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RISS 인기검색어
- 검색키워드
- 저자 : Khalil TALEBI (검색결과 3 건)
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Effects of essential oil from Teucrium polium on some digestive enzyme activities of Musca domestica
Mahdieh BIGHAM,Vahid HOSSEININAVEH,Banafsheh NABAVI,Khalil TALEBI,Naimeh-Sadat ESMAEILZADEH 한국곤충학회 2010 Entomological Research Vol.40 No.1
The essential oil from Teucrium polium was evaluated for its adverse effects on larval instars of Musca domestica. The essential oil was blended with a diet at a concentration that produced 50% mortality of subjected insects (LC50) (80 ppm). To learn about the situation of digestive enzymes of M. domestica treated with the essential oil, third larval instars were dissected under non-active enzyme conditions and their midguts were removed. The supernatant was used as an enzyme source after homogenization and centrifugation of the homogenates. Results revealed that some main digestive enzymes in the larval midgut were adversely affected when exposed to the food-incorporated essential oil. Proteinase extracted from larval midgut hydrolyzed the synthetic substrates B-Arg-pNA, Z-Arg-Arg-PNA and Z-Phe-Arg-PNA for trypsin, cathepsin B and cathepsin L activities, respectively, in control and treated larvae. The essential oil caused a reduction of 61.5% in tryptic activity. Significant 69% and 79% reductions were also observed in cathepsin L and B activities, respectively. Carbohydrase activities of α-amylase, α-glucosidase and β-glucosidase were detected in larval midgut extract. All assayed carbohydrases were affected by the essential oil. The most notable impact, a 93% reduction, was observed in α-amylase. Decreases of 69.5% and 42% were obtained in α-glucosidase and β-glucosidase activity, respectively. This study indicated that the larvicidal effect of the essential oil from Teucrium polium may be due to its detrimental effects on digestive enzymes. It seems that the detrimental effect of the oil can be due to both the inhibitory nature of the oil and the destruction of the midgut epithelium.
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Delkash‑Roudsari Sahar,Hossein Goldansaz Seyed,Talebi-Jahromi Khalil,Abramson Charles I. 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1
Insecticides are a major tool for controlling pest species. Their widespread use results in damage to non-targeted insects, with honey bees particularly at risk. During foraging, honey bees learn and remember floral charac teristics that are associated with food. As insect pollinators, honey bees inadvertently contact chemicals which can have multiple negative impacts. The toxicity of two insecticides from different classes, ethion (47.79 mg a.i. L − 1 ) and hexaflumuron (500 mg a.i.L − 1 ), on learning, memory, and sensory perception were evaluated. We found that oral exposure to ethion had adverse effects on learned proboscis extension toward reward-associated odors and colors. In addition, we showed reduced sucrose consumption and sucrose responsiveness after expo sure. Hexaflumuron also impaired olfactory learning and memory and decreased responsiveness to sucrose and water. Exposure to sub-lethal concentration of the cholinergic organophosphate insecticide, ethion (47.79 mg a.i. L − 1 ), and the field-recommended concentration of hexaflumuron (500 mg a.i.L − 1 ), significantly impaired behavior involved in foraging. Our results suggest that several behavioral characteristics of honey bees be evaluated when testing an insecticide rather than relying on just one behavioral measure.
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Parvin Razavi Tabatabaei,,Vahid Hosseininaveh,Seyed Hossein Goldansaz,Khalil Talebi 한국응용곤충학회 2011 Journal of Asia-Pacific Entomology Vol.14 No.2
Digestive proteinases and carbohydrases of Ectomyelois ceratoniae (Zeller) larvae were investigated using appropriate substrates and inhibitors. Midgut pH in larvae was determined to be slightly alkaline. Midgut extracts showed optimum activity for proteolysis of hemoglobin at pH 9–12. Midgut proteinases also hydrolyzed the synthetic substrates of trypsin, chymotrypsin, and elastase at pH 8–11. Maximum digestive α-amylase activity was also observed at pH 8–11. However, optimum activity for α- and β-glucosidase occurred at pH 5–8. Alpha- and β-galactosidases optimum activities occurred at pH 5 and pH 6, respectively. Inhibitors of serine proteases were effective on midgut serine proteases (trypsin and chymotrypsin proteases). Zymogram analyses revealed at least five bands of total proteolytic activity in the larval midgut. Proteasespecific zymogram analyses revealed at least four, two, and one isozymes for trypsin-, chymotrypsin-, and elastase-like activities respectively. Two α-amylase isozymes were found in the midgut of fifth instar larvae and in the whole bodies of 1st through 5th instar larvae. Zymogram studies also revealed the presence of one and two bands of activity for β- and α-glucosidase, respectively. Recycling of α-amylase and proteases in the larval midgut was not complete. At least one isozyme of trypsin, chymotrypsin, elastase, and α-amylase were not recycled and were observed in the larval hindgut.
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