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Madhu Bala,Devinder Singh 한국곤충학회 2015 Entomological Research Vol.45 No.4
The development of the Oriental latrine fly, Chrysomya megacephala (Fabricius), and hairy maggot blowfly, C. rufifacies (Macquart) (Diptera: Calliphoridae), was studied at four different temperatures (22°C, 25°C, 29°C and 31°C) in order to draw correlations between larval age, body length and body dry weight. The mean larval body length increased steadily from a minimum of 1.4 mm for C. megacephala and 1.8 mm for C. rufifacies to a maximum of 17.4 mm for C. megacephala and 15.9 mm for C. rufifacies at different temperatures. Similarly, the mean dry weight increased steadily from a minimum of 0.0007 g for C. megacephala (second instar) and 0.0008 g for C. rufifacies (second instar) to a maximum of 0.0290 g for C. megacephala and 0.0270 g for C. rufifacies at different temperatures. Entomological evidence is often used to estimate the minimum postmortem interval (mPMI) and both of these species are important from a forensic point of view. Graphs of age of larvae vs. body length and age of larvae vs. dry body weight at different temperatures can be used to estimate the larval age of these two species.
Devinder Singh,Madhu Bala 한국곤충학회 2011 Entomological Research Vol.41 No.2
Survival of larvae of known ages after 2 to 6 h of submersion under water was assessed by observing pupation and adult emergence in the cases of two blowflies of forensic importance, Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart). Lowest survival occurred among 10-h-old larvae, which could not tolerate more than 2 h of submergence. Even with such a small period of submergence success of survival was only 33%. There were no survivors when 20- and 30-h-old larvae were submerged for more than 3 and 4 h, respectively. In the cases of 40-, 50-, 60- and 70-h-old larvae there were no survivors if submergence was more than 5 h. The results show that there is an inverse relationship between submergence period and survival rate: the longer the submergence period, the lesser is the survival rate. This study provides data that are potentially useful in estimating the minimum time since submergence (TSS) of a corpse during forensic investigations.
Radioprotection by Rhodiola imbricata in Mice Against Whole-Body Lethal Irradiation
H.C. Goel,Madhu Bala,J. Prasad,S. Singh,P.K. Agrawala,R.C. Swahney 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.2
Rhodiola imbricata, an Indian medicinal plant, was investigated for protection against whole-body lethal gammairradiation (10 Gy)-induced mortality in Swiss albino strain “A” mice. The maximum tolerance dose values for aqueous (RD-I) and aqua-alcoholic (RD-II) extracts were 1,100 and 1,300 mg/kg of body weight, respectively. Pre-irradiation admin-istration of RD-I produced . 90% survival, while RD-II produced . 83% survival beyond the 30-day observation period. Theoptimal radioprotective dose for RD-I as well as RD-II was 350 mg/kg of body weight; the aqua-alcoholic extract, however,had an advantage over the aqueous extract at lower as well as at higher doses. The optimal time interval between adminis-tration of extract and irradiation was 30 minutes for both RD-I and RD-II. The number of colony-forming units per spleen inirradiated mice was 1.91. 0.15, while in mice given RD-I or RD-II, 30 minutes before irradiation (10 Gy), it increased to17.3. 0.67 and 15.6. 0.61, respectively. These findings have important implications in the development of a suitable ra-dioprotector of herbal origin.
Enzyme washing of indigo and sulphur dyed denim
A. K. Patra,Amit Madhu,Neeraj Bala 한국의류학회 2018 Fashion and Textiles Vol.5 No.1
A wide ranging finish effects have been given to denim fabrics in industrial scale to make it more fashionable and functional. Among the numerous wet and dry treatments given, worn out looks of various kinds have received the maximum attention. In this context, herein, two denim fabrics, one dyed with vat indigo and the other with sulphur black were enzyme washed. The enzymes used were acid cellulase and neutral cellulase and the treatments were done at varying concentrations, time and mechanical agitation. The trials so taken were based on design of experiments, and the effect was analysed in terms of decrease in colour depth and weight loss. Back staining of fabric during the wash was also checked and acid enzyme despite higher colour removal, caused significant staining in sulphur dyed quality. Attempt was made to contain this back staining by suitable treatment. The application conditions of cellulases on the denim qualities were also optimized for fading effect.