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- 저자 : Ma Qingqing (검색결과 2 건)
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Gu Cuihua,Hong Sidan,Wang Jie,Shang Linxue,Zhang Guozhe,Zhao Yu,Ma Qingqing,Ma Dandan 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.1
Lagerstroemia indica is a summer ornamental fl owering tree, belonging to the Lythraceae family. Its fl ower color is a key ornamental characteristic. Recent research indicates that WRKY and bZIP transcription factors play important role in f l ower color formation. Based on the transcriptome database of L. indica , this study identifi ed 85 LibZIPs and 61 LiWRKYs from the L. indica transcriptome database and analyzed their expression profi les using qRT-PCR. Phylogenetic analysis divided LibZIPs into 11 subfamilies and LiWRKYs into six subfamilies. Nine LibZIPs and four LiWRKYs were found to have higher expression in colored samples compared to the white sample. LibZIP31 , LibZIP65 , LibZIP34 , LiWRKY16 , LiWRKY25 , LiWRKY40 , and LiWRKY67 may play important roles in fl ower color formation in L. indica . These results provide valuable information for further functional analysis of the bZIP and WRKY genes families in L. indica and help clarify their roles in fl ower color formation.
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Songyi Zhou,Yizhao Pan,Yan Zhang,Lijun Gu,Leikai Ma,Qingqing Xu,Weijian Wang,Jiehao Sun 대한통증학회 2023 The Korean Journal of Pain Vol.36 No.3
Background: Spinal N-methyl-D-aspartate (NMDA) receptor activation is attributed to remifentanil-induced hyperalgesia (RIH). However, the specific mechanism and subsequent treatment is still unknown. Previous studies have shown that the dynamin-related protein 1 (DRP1)-mitochondria-reactive oxygen species (ROS) pathway plays an important role in neuropathic pain. This study examined whether antisense oligodeoxynucleotides against DRP1 (AS-DRP1) could reverse RIH. Methods: The authors first measured changes in paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) at 24 hours before remifentanil infusion and 4, 8, 24, and 48 hours after infusion. The expression levels of DRP1 and NR2B were measured after behavioral testing using Western blotting. In addition, DRP1 expression was knocked down by intrathecal administration of AS-DRP1 to investigate the effects of DRP1 on RIH. The behavioral testing, the expression levels of spinal DRP1 and NR2B, and dorsal mitochondrial superoxide were measured. Changes in mitochondrial morphology were assessed using electron microscopy. Results: After remifentanil exposure, upregulation of spinal DRP1 and NR2B was observed along with a reduction in PWMT and PWTL. In addition, AS-DRP1 improved RIH-induced PWTL and PWMT (P < 0.001 and P < 0.001) and reduced remifentanil-mediated enhancement of spinal DRP1 and NR2B expression (P = 0.020 and P = 0.022). More importantly, AS-DRP1 reversed RIH-induced mitochondrial fission (P = 0.020) and mitochondrial superoxide upregulation (P = 0.031). Conclusions: These results indicate that AS-DRP1 could modulate NMDA receptor expression to prevent RIH through the DRP1-mitochondria-ROS pathway.
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