Experimental Pathogenesis of Pullorum Disease with the Local Isolate of Salmonella enterica serovar. enterica subspecies Pullorum in Pullets in Bangladesh

Haider, M.G.,Chowdhury, E.H.,Khan, M.A.H.N.A.,Hossain, M.T.,Rahman, M.S.,Song, H.J.,Hossain, M.M. The Korean Society of Poultry Science 2009 韓國家禽學會誌 Vol.35 No.4

이 실험은 추백리의 병원성을 연구하고자 2006년 2월부터 12월까지 Salmonella enterica serovar. enterica subspecies (S.) Pullorum 야외주를 분리한 후, 이를 건강한 닭에 실험적으로 감염시킨 다음 임상증상, 여러 기관의 육안 및 조직병리학적 검색과 아울러 공격주의 재분리와 동정을 시도하였다. S. Pullorum에 혈청학적으로 음성인 12주령의 100수의 암탉을 $A{\sim}E$까지 20수씩 5그룹으로 구분하였다. $A{\sim}DS$. Pullorum을 $10^6\;CFU$, $10^7\;CFU$, $2{\times}10^7\;CFU$, $10^8\;CFU$로 각각 경구 감염시켰고, E는 비감염 대조군으로 삼았다. 실험 방법으로는 부검, 조직병리학적 검사, Salmonella에 대한 세균 배양, 염색, 생화학적 특성을 조사하고 그 결과를 기술하였다. The research work was carried out to study the pathogenesis covering the clinical signs, gross and histopathological lesions in different organs, and reisolation and identification of the organisms after experimental infection with the local isolate of Salmonella enterica serovar. enterica subspecies (S.) Pullorum at different time interval of the experiment during the period February 2006 to December 2006. One hundred pullets (seronegative to S. Pullorum of 12 weeks age were purchased and divided into 5 (A, B, C, D and E) groups and each group consisted of 20 birds. Four groups (A, B, C and D) were infected orally with a dose of $10^6\;CFU$, $10^7\;CFU$, $2{\times}10^7\;CFU$, $10^8\;CFU$ of S. Pullorum, respectively, and one group (E) was treated as uninfected control. The used methods were necropsy and histopathology, culture of bacteria, staining and biochemical test of Salmonella. Five birds from each group were randomly selected and sacrificed $1^{st}$ week, $2^{nd}$, $3^{rd}$ and $4^{th}$ weeks of post infection (PI). From all the groups, the bacteriological samples (crop, liver, lung, heart, spleen, bile duodenum, ceca and blood) were collected with pre enriched in buffered peptone water in sterile poly bags. Liver, lungs, heart, spleen, intestine, etc. were collected in 10% buffered-formalin for histopathological examination. No clinical signs, gross and histopathological lesions were found in control group and no S. Pullorum was reisolated. Clinical sign of experimentally infected with S. Pullorum in pullets were loss of appetite (100%), slight depression (75%), ruffled feathers (85%), diarrhea (60%) and loss of weight (100%) in chickens. The feed intake and body weight at different weeks after PI differed significantly (p<0.01) among the groups. Grossly, the highest recorded lesion was button-like ulcer in the ceca (80%) and the lowest was white nodules in lungs (1.25%). S. Pullorum were reisolated from crop (91.25%), liver (91.25%), lung (83.75%), heart (71.25%), spleen (87.75%), bile (33.25%), duodenum (92.50%), ceca (97.50%) and from different group of infection (61.25%). The highest microscopic findings were intestinal and cecal mucosa and submucosa exhibited infiltration of mononuclear cells and congestion (96.25%), and the lowest finding was nodule formation in the lungs (3.75%). The pattern of the disease production by local isolate of S. Pullorum in Bangladesh is almost similar with other isolates in different countries.

Experimental Pathogenesis of Pullorum Disease with the Local Isolate of Salmonella enterica serovar. enterica subspecies Pullorum in Pullets in Bangladesh

M. G. Haider,E. H. Chowdhury,M. A. H. N. A. Khan,M. T. Hossain,M. S. Rahman,송희종,M. M. Hossain 한국가금학회 2008 韓國家禽學會誌 Vol.35 No.4

The research work was carried out to study the pathogenesis covering the clinical signs, gross and histopathological lesions in different organs, and reisolation and identification of the organisms after experimental infection with the local isolate of Salmonella enterica serovar. enterica subspecies (S.) Pullorum at different time interval of the experiment during the period February 2006 to December 2006. One hundred pullets (seronegative to S. Pullorum of 12 weeks age were purchased and divided into 5 (A, B, C, D and E) groups and each group consisted of 20 birds. Four groups (A, B, C and D) were infected orally with a dose of 106 CFU, 107 CFU, 2 × 107 CFU, 108 CFU of S. Pullorum, respectively, and one group (E) was treated as uninfected control. The used methods were necropsy and histopathology, culture of bacteria, staining and biochemical test of Salmonella. Five birds from each group were randomly selected and sacrificed 1st week, 2nd, 3rd and 4th weeks of post infection (PI). From all the groups, the bacteriological samples (crop, liver, lung, heart, spleen, bile duodenum, ceca and blood) were collected with pre enriched in buffered peptone water in sterile poly bags. Liver, lungs, heart, spleen, intestine, etc. were collected in 10% buffered-formalin for histopathological examination. No clinical signs, gross and histopathological lesions were found in control group and no S. Pullorum was reisolated. Clinical sign of experimentally infected with S. Pullorum in pullets were loss of appetite (100%), slight depression (75%), ruffled feathers (85%), diarrhea (60%) and loss of weight (100%) in chickens. The feed intake and body weight at different weeks after PI differed significantly (p<0.01) among the groups. Grossly, the highest recorded lesion was button-like ulcer in the ceca (80%) and the lowest was white nodules in lungs (1.25%). S. Pullorum were reisolated from crop (91.25%), liver (91.25%), lung (83.75%), heart (71.25%), spleen (87.75%), bile (33.25%), duodenum (92.50%), ceca (97.50%) and from different group of infection (61.25%). The highest microscopic findings were intestinal and cecal mucosa and submucosa exhibited infiltration of mononuclear cells and congestion (96.25%), and the lowest finding was nodule formation in the lungs (3.75%). The pattern of the disease production by local isolate of S. Pullorum in Bangladesh is almost similar with other isolates in different countries. The research work was carried out to study the pathogenesis covering the clinical signs, gross and histopathological lesions in different organs, and reisolation and identification of the organisms after experimental infection with the local isolate of Salmonella enterica serovar. enterica subspecies (S.) Pullorum at different time interval of the experiment during the period February 2006 to December 2006. One hundred pullets (seronegative to S. Pullorum of 12 weeks age were purchased and divided into 5 (A, B, C, D and E) groups and each group consisted of 20 birds. Four groups (A, B, C and D) were infected orally with a dose of 106 CFU, 107 CFU, 2 × 107 CFU, 108 CFU of S. Pullorum, respectively, and one group (E) was treated as uninfected control. The used methods were necropsy and histopathology, culture of bacteria, staining and biochemical test of Salmonella. Five birds from each group were randomly selected and sacrificed 1st week, 2nd, 3rd and 4th weeks of post infection (PI). From all the groups, the bacteriological samples (crop, liver, lung, heart, spleen, bile duodenum, ceca and blood) were collected with pre enriched in buffered peptone water in sterile poly bags. Liver, lungs, heart, spleen, intestine, etc. were collected in 10% buffered-formalin for histopathological examination. No clinical signs, gross and histopathological lesions were found in control group and no S. Pullorum was reisolated. Clinical sign of experimentally infected with S. Pullorum in pullets were loss of appetite (100%), slight depression (75%), ruffled feathers (85%), diarrhea (60%) and loss of weight (100%) in chickens. The feed intake and body weight at different weeks after PI differed significantly (p<0.01) among the groups. Grossly, the highest recorded lesion was button-like ulcer in the ceca (80%) and the lowest was white nodules in lungs (1.25%). S. Pullorum were reisolated from crop (91.25%), liver (91.25%), lung (83.75%), heart (71.25%), spleen (87.75%), bile (33.25%), duodenum (92.50%), ceca (97.50%) and from different group of infection (61.25%). The highest microscopic findings were intestinal and cecal mucosa and submucosa exhibited infiltration of mononuclear cells and congestion (96.25%), and the lowest finding was nodule formation in the lungs (3.75%). The pattern of the disease production by local isolate of S. Pullorum in Bangladesh is almost similar with other isolates in different countries.

Aminated polyethersulfone-silver nanoparticles (AgNPs-APES) composite membranes with controlled silver ion release for antibacterial and water treatment applications

Haider, M.S.,Shao, G.N.,Imran, S.M.,Park, S.S.,Abbas, N.,Tahir, M.S.,Hussain, M.,Bae, W.,Kim, H.T. Elsevier 2016 Materials Science and Engineering C Vol.62 No.-

<P>The present study reports the antibacterial disinfection properties of a series of silver nanoparticle (AgNP) immobilized membranes. Initially, polyethersulfone (PES) was functionalized through the introduction of amino groups to form aminated polyethersulfone (NH2-PES, APES). AgNPs were then coordinately immobilized on the surface of the APES composite membrane to form AgNPs-APES. The properties of the obtained membrane were examined by FT-IR, XPS, XRD, TGA, ICP-OES and SEM-EDAX analyses. These structural characterizations revealed that AgNPs ranging from 5 to 40 nm were immobilized on the surface of the polymer membrane. Antibacterial tests of the samples showed that the AgNPs-APES exhibited higher activity than the AgNPs-PES un-functionalized membrane. Generally, the AgNPs-APES 1 cm x 3 cm strip revealed a four times longer life than the un-functionalized AgNPs polymer membranes. The evaluation of the Ag+ leaching properties of the obtained samples indicated that approximately 30% of the AgNPs could be retained, even after 12 days of operation. Further analysis indicated that silver ion release can be sustained for approximately 25 days. The present study provides a systematic and novel approach to synthesize water treatment membranes with controlled and improved silver (Ag+) release to enhance the lifetime of the membranes. (C) 2016 Elsevier B.V. All rights reserved.</P>

Experimental Pathogenesis of Pullorum Disease with the Local Isolate of Salmonella enterica serovar. enterica subspecies Pullorum in Pullets in Bangladesh

M. G. Haider,E. H. Chowdhury,M. A. H. N. A. Khan,M. T. Hossain,M. S. Rahman,H. J. Song,M. M. Hossain 한국가금학회 2009 韓國家禽學會誌 Vol.35 No.4

농용 회전익기 로터 양력의 현장측정과 CFD 시뮬레이션 비교

홍종근 ( J. G. Hong ),구영모 ( Y. M. Koo ),하이더바샤랏알리 ( B. A. Haider ),손창현 ( C. H. Sohn ) 한국농업기계학회 2017 한국농업기계학회 학술발표논문집 Vol.22 No.2

농용 회전익기의 로터 블레이드는 익형의 설계를 통하여 양력을 향상시킬 수 있다(Koo, 2012). 본 연구의 목적은 개발된 익형을 장착한 농용 헬리콥터(AGROHELI_4G)의 양력 특성을 자유형 양력시험장치를 이용하여 측정하는데 있어 지면에 앵커된 현장실험 조건을 반영한 CFD 시뮬레이션을 수행하고 현장실험의 결과와 비교하는데 있다. 현장 및 모의실험결과는 제자리 또는 서행조건으로 확장하기 위하여 현장실험과 근사한 조건에서 총양력을 예측하여 검증하였다. 시뮬레이션은 MRF (multiple reference frame) 모델을 채용하여 유동영역을 구성하였으며, 경계층을 wall로 설정한 지면바닥으로부터 블레이드를 0.7R (R: 블레이드 반경)의 높이에 위치시켜 지면효과(ground effect)를 시뮬레이션에 적용하였다. 로터를 둘러싸고 있는 원통 모양의 유동장은 20R로서 정지역(stationary zone)과 운동역(moving zone)으로 구성되어 있고 운동역과의 경계조건은 Interface로 유체의 유입 및 유출이 가능하도록 하였고, 18,000~20,000 iteration 사이에서 양력의 표준편차가 5% 정도까지 수렴하도록 수행하였다. 자유형 양력 시험 장치를 이용하여 현장 측정한 결과 조정간 위치 신호는 피치각과 엔진 스로틀이 연동되어 피치각 5~10도에서 로터의 회전속도는 600~1000 rpm의 범위에서 연동되었다(Koo et al., 2016). 조정간의 신호를 환산한 CPA와 스로틀의 연동으로 결정된 로터 회전속도의 연동에 따른 양력의 비교에서 CPA 8°에서 자중(68 kgf)을 극복하고 유상하중(payload)의 양력을 발휘하기 시작하였다. CPA 10°에서는 유상하중 60 kg을 보이며 총양력 128 kg을 발휘하였다. 실제 현장에서의 CPA의 운용범위는 실속각(stall angle: 약 22°)에 훨씬 못 미치는 각도이며, 본 연구에서 사용된 24.5 kW의 엔진동력은 약 12°의 CPA 이하에서 사용이 제한되었다. 시뮬레이션 결과에서는 CPA 약 8°에서 자중을 극복하고 유상하중(payload)을 발휘하기 시작하였으며, CPA 10°에서의 총양력은 1,266 N으로 유상하중이 61.2 kg로 예측되어 양력 실험치보다 약 2% 높게 예측되었다. CPA 10°에서의 시뮬레이션 세부결과는 양력 1,266 N, 항력 127.2 N, 토크 147.9 N · m, 동력은 15.5kW로 나타났으므로, 현장에서의 유상하중 측정치와 지면효과를 적용한 시뮬레이션의 결과는 2%의 오차에서 일치하였다.

Practical payload assessment of a prototype blade for agricultural unmanned rotorcraft

Koo, Y. M.,Hong, J. G.,Haider, B. A.,Sohn, C. H. 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.12

Sol-gel synthesis of TiO₂-Fe₂O₃ systems: Effects of Fe₂O₃ content and their photocatalytic properties

Abbas, N.,Shao, G.N.,Haider, M.S.,Imran, S.M.,Park, S.S.,Kim, H.T. Korean Society of Industrial and Engineering Chemi 2016 Journal of industrial and engineering chemistry Vol.39 No.-

<P>An inexpensive sol-gel technique to synthesize TiO2-Fe2O3 nanocomposites with improved structural and photochemical properties is introduced. A series of TiO2-Fe2O3 nanocomposites with different Fe2O3 contents were prepared through cheap TiO2 precursor titanium oxychloride (TiOCl2). The physicochemical properties of the samples were examined by XRD, XRF, SEM, EDX, HRTEM, UV-vis DRS, FTIR, TGA and nitrogen gas physisorption studies. The effects of calcination temperatures and Fe2O3 content on the mesostructure and photocatalytic strength of the prepared TiO2-Fe2O3 composites were investigated. Results showed that composites with distinct structural, optical and photochemical properties can be formed by varying Fe2O3 content. (C) 2016 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.</P>

Inexpensive synthesis of a high-performance Fe3O4-SiO2-TiO2 photocatalyst: Magnetic recovery and reuse

Abbas, N.,Shao, G. N.,Imran, S. M.,Haider, M. S.,Kim, H. T. Springer Science and Business Media 2016 Frontiers of chemical science and engineering Vol.10 No.3

<P>A sol-gel technique has been developed for the synthesis of a magnetite-silica-titania (Fe3O4-SiO2-TiO2) tertiary nanocomposite with improved photocatalytic properties based on the use of inexpensive titania and silica precursors. The exceptional photocatalytic activity of the resulting materials was demonstrated by using them to photocatalyze the degradation of methylene blue solution. The best formulation achieved 98% methylene blue degradation. An interesting feature of the present work was the ability to magnetically separate and reuse the catalyst. The efficiency of the catalyst remained high during two reuses. The synthesized nanomaterials were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, ultra-violet-visible spectroscopy, diffuse reflectance spectroscopy, and thermogravimetric analysis. XRD analysis revealed the formation of multicrystalline systems of cubic magnetite and anatase titania crystals. SEM and TEM characterization revealed well-developed and homo-geneously dispersed particles of size less than 15 nm. FTIR spectra confirmed the chemical interaction of titania and silica. It was further noticed that the optical properties of the prepared materials were dependent on the relative contents of their constituent metal oxides.</P>

해외저술 소개 : 임상 연구: 진단 ; 대장직장암으로부터의 간전이로 인한 간내담관의 확장(초)

( Jhaveri K. S. ),( Halankar J. ),( Aguirre D. ),( Haider M. ),( Lockwood G. ),( Guindi M. ),( Fischer S. ) 대한간암연구회 2010 대한간암학회지 Vol.10 No.-

Escherichia coli septicemia concurrent with mycotic infection in captive salt water crocodiles in Bangladesh

( Sajeda Sultana ),( Emdadul H. Chowdhury ),( R. Parvin ),( Shib S. Saha ),( Sheik M. Rahman ),( M. G. Haider ),( Abu S. M. Arif ),( Siddiqur Rahman ),( Hee Jong Song ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.1

some crocodiles were found sick and three of them died between May and July in 2006. This investigation was performed to diagnose the cause of the death. Routine postmortem examination was conducted. Samples were collected in 10% neutral buffered formalin for histopathology and in falcon tube for microbiological study. Additional swabs were collected in nutrient broth. Histopathological and microbiological studies were conducted using routine procedures. In addition Giemsa, Gram and PAS stains were performed to detect the organism in tissues. Grossly, esophagus, trachea, lungs, liver, spleen, heart and kidney were congested. Intestine, rectum and colon were hemorrhagic. Clay colored material was found in colo-rectum. Purulent exudates in lungs and thick and cloudy pericardial fluid in pericardial sac were found. Histologically, multifocal granulomatous inflammation was evident in lung, liver, kidney, intestine and colon with bacterial colonies, fungal spores and hyphae. These bacteria were appeared as Gram negative. Fungal hyphae and spores were detected in liver, lungs and colon by using PAS stain. Bacteriologically, E. coli were isolated from lungs exudates, pericardial fluids and intestinal fluids. Therefore, it can be concluded that 3 crocodiles died due to E. coli septicemia concurrent with mycotic infection.