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- 저자 : Luan Aiping (검색결과 1 건)
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Xie Tao,Zhang Wei,Chen Chengjie,Wang Xiaoshuang,Zhang Jing,Luan Aiping,He Yehua 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.6
Somatic embryogenesis (SE) is a key regeneration process in plant. AcSERK1 is a gene specifi cally expressed in the early stage of SE in pineapple ( Ananas comosus ), suggesting that the promoter of SERK1 might contain specifi c cis acting element regulating SE. To identify embryonic cell-specifi c element in the SERK1 promoter, a series of binary plant transformation vectors with GUS (β-glucuronidase) reporter gene were systematically analyzed by transient gene expression system in wild-type and transgenic pineapple embryogenic callus. Histochemical and quantitative GUS assays demonstrated that the activity of the AcSERK1 upstream regulatory sequence lacking − 921 to -911 or -910 to -880 was signifi cantly reduced in the embryonic callus of the pineapple, and these two regions were needed for the embryonic cell-specifi c. Besides, a promoter lacking − 943 to -922 was shown to signifi cantly increase GUS activity in embryogenic callus, suggesting repressive elements exist in this region. Our data of stable transformation assays confi rmed again the 5’ upstream regulatory sequence (-921 to -880) of the AcSERK1 gene is an essential functional region. Our fi ndings lay the basis for better understanding of the molecular mechanisms of AcSERK1 gene in the regulation in early stage of SE.
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