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Kyungwon Min(민경원),Liu Bing(리우 빙),Sang-Ryong Lee(이상용),Rajeev Arora(라지브 아로라) 환경독성보건학회 2021 한국독성학회 심포지움 및 학술발표회 Vol.2021 No.5
Supplemental calcium (Ca2+)fertilization has been reported to enhance plant tolerance against various abiotic stresses, however, its effect on freezing tolerance has not been well studied. We investigated the effect of Ca2+supplementation on freezing tolerance of spinach (Spinacia oleracea L.) at whole-plant as well as excised-leaf level. Seedlings supplemented with 20 and 25 mM Ca2+ through sub-irrigation efficiently assimilated Ca2+, evident by higher leaf-Ca2+ content. Supplemental Ca2+ did not impede leaf-growth(leaf-area) but slightly increased dry weight / fresh weight ratio compared to the control. Supplemental Ca2+ significantly improved freezing tolerance as evident by reducing K+/Mg2+/total ion-leakage (i.e. indicative of less membrane damage), alleviating oxidative stress (less accumulation of O2 and H2O2), and enhancing PSII potential quantum yield /energy trapping efficiency (Fv/Fm) following a relatively moderate or severe freeze-thaw stress, i.e., -5.5 and -6.5 ˚C. Improved freezing tolerance by Ca2+ supplementation was paralleled by enhanced activities of antioxidant enzymes, super oxide dismutase (SOD), catalase(CAT), and ascorbate peroxidase (APX). Our data further suggests that, besides abolstered antioxidant status, improved freezing tolerance of Ca2+-fed seedlings may also be associated with reduced loss of Ca2+ from plasma membrane (due to lower abundance of leaked K+ in extra cellular spaces) as well as due to increased mechanical strength of cell wall as evident by cell wall augmentation.