http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Lippitz, Andreas (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Park, Jisoo,Baik Kim, Gae,Lippitz, Andreas,Kim, Young Mi,Jung, Donggeun,Unger, Wolfgang E.S.,Kim, Young-Pil,Lee, Tae Geol Elsevier 2019 Sensors and actuators. B Chemical Vol.281 No.-
<P><B>Abstract</B></P> <P>We report polyethylene glycol (PEG)-grafting antifouling surfaces using a plasma copolymerized (PcP) technique to monitor protease activity in complex media. By varying the mixing ratio of the PEG and ethylenediamine (EDA) precursors, the PcP-PEG-EDA (PcP-PE) film was able to easily control surface amine density with good preservation of the internal PEG structure. We found that nonspecific protein adsorption was dramatically reduced in serum-containing media on the PcP-PE films, as opposed to that on plasma polymerized-EDA (PP-E) films without PEG. When SPR sensor chips coated with PcP-PE film were employed to detect protease activity, biotinylated luciferase probes (luciferase-peptide-biotin) on streptavidin-conjugated SPR chips enabled real-time and label-free measurement of matrix metalloproteinase activity in cell culture media. Owing to its excellent antifouling ability, this newly developed method boasts minimal nonspecific binding and can serve as a biochip platform to promote a wide range of applications in the biological field.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Plasma copolymerized (PcP) method with PEG/EDA created antifoulding surfaces. </LI> <LI> Protease activity in cell culture media was detected on a PcP/SPR sensor chip. </LI> <LI> This approach will be useful for bioassays with minimal nonspecific binding. </LI> </UL> </P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
