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RISS 인기검색어
- 검색키워드
- 저자 : Lintao Zhang (검색결과 3 건)
- 무료
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Parametric investigation on an industrial electromagnetic continuous casting mould performance
Lintao Zhang,Anyuan Deng,Ian Cameron,Engang Wangb,Johann Sienz 한국CDE학회 2017 Journal of computational design and engineering Vol.4 No.4
This research aimed at conducting a quantitative investigation of process parameters on the magnetic field contribution in an electromagnetic continuous casting mould. The Taguchi method (4 factors and 3 factor value levels: L9 orthogonal array) was adopted to design matrix of the simulation runs and the analysis of variance was used to evaluate the contributions of each control factor. The simulations were conducted based on the finite element method and the numerical set-up was validated by the designed experiment. The results showed that the applied alternating current magnitude contributed most (76.64%) to the magnetic field level in the mould, compared to the other control factors. It was followed by the slit length (17.72%), the alternating current frequency (4.17%) and the slit width (1.57%).
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An Improved Method for Rapid Preparation of Oligodendrocytespecific Rabbit Polyclonal Antibody
Tao Wang,Wei Wang,Lintao Jia,Xin Bu,Fang Wang,Libo Yao,Bin Zhang 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.3
Antibodies are important tools in the study of protein function and diagnostic tests. However, traditional antiserum preparation requires a time-consuming immunization protocol and subsequent purification of polyclonal antibodies. In this study, a rapid and efficient method for polyclonal antibody preparation has been developed. Juxtanodin (JN) and silent information regulator-2 (Sirt2),both of which are oligodendrocyte-specific proteins, were used for antibody preparation. The N-terminal 170 amino acids of JN (JN170) and amino acids 231-351 of Sirt2 (Sirt2-121) were expressed as GST-tagged proteins from a pET-41a(+) vector in E. coli strain BL21 (DE3) cells. The fusion proteins were purified and used to immunize rabbits following both a traditional protocol, in which antigen was presented biweekly, and a modified rapid protocol, in which the immunization on day 1 was boosted on days 5and 28. ELISA, Western blot analysis and immunofluorescent staining showed that antibodies produced via the rapid protocol could recognize these two oligodendrocytespecific proteins in vitro and in the rat central nervous system (CNS), respectively, similar to those produced with the traditional protocol. Thus, our study provides a novel rapid method to prepare high specificity antibodies via a modified immunization protocol and subsequent antibody purification.
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Effects of sorbitol on porcine oocyte maturation and embryo development <i>in vitro</i>
Lin, Tao,Zhang, Jin Yu,Diao, Yun Fei,Kang, Jung Won,Jin, Dong-Il Cambridge University Press 2015 Zygote Vol.23 No.2
<B>Summary</B><P>In the present study, a porcine system was supplemented with sorbitol during <I>in vitro</I> maturation (IVM) or <I>in vitro</I> culture (IVC), and the effects of sorbitol on oocyte maturation and embryonic development following parthenogenetic activation were assessed. Porcine immature oocytes were treated with different concentrations of sorbitol during IVM, and the resultant metaphase II stage oocytes were activated and cultured in porcine zygote medium-3 (PZM-3) for 7 days. No significant difference was observed in cumulus expansion and the nuclear maturation between the control and sorbitol-treated groups, with the exception of the 100 mM group, which showed significantly decreased nuclear maturation and cumulus expansion. There was no significant difference in the intracellular reactive oxygen species (ROS) levels between oocytes matured with 10 or 20 mM sorbitol and control groups, but 50 and 100 mM groups had significantly higher ROS levels than other groups. The 20 mM group showed significant increases in intracellular glutathione and subsequent blastocyst formation rates following parthenogenetic activation compared with the other groups. During IVC, supplementation with sorbitol significantly reduced blastocyst formation and increased the apoptotic index compared with the control. The apoptotic index of blastocysts from the sorbitol-treated group for entire culture period was significantly higher than those of the partially sorbitol-exposed groups. Based on these findings, it can be concluded that the addition of a low concentration of sorbitol (20 mM) during IVM of porcine oocytes benefits subsequent blastocyst development and improves embryo quality, whereas sorbitol supplement during IVC has a negative effect on blastocyst formation.</P>
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