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Lim, Joon Seok,Kim, Daehong,Baek, Song-Ee,Myoung, Sungmin,Choi, Junjeong,Shin, Sang Joon,Kim, Myeong-Jin,Kim, Nam Kyu,Suh, Jinsuk,Kim, Ki Whang,Keum, Ki Chang Springer International 2012 EUROPEAN RADIOLOGY Vol.22 No.8
<P>To evaluate the utility of perfusion MRI as a potential biomarker for predicting response to chemoradiotherapy (CRT) in locally advanced rectal cancer.</P>
Kyu Hee Lim,Ji-Hui Han,Yoon Seok Roh,Bumseok Kim,Jung-Kee Kwon,Myoung Jo You,Ho Jae Han,Sohail Ejaz,Chang-Won Kang,Jong-Hoon Kim 대한생리학회-대한약리학회 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.3
Natural killer (NK) cells provide one of the initial barriers of cellular host defense against pathogens, in particular intracellular pathogens. Because bone marrow-derived hematopoietic stem cells (HSCs), lymphoid protenitors, can give rise to NK cells, NK ontogeny has been considered to be exclusively lymphoid. Here, we show that porcine c-kit<sup>+</sup> bone marrow cells (c-kit<sup>+</sup> BM cells) develop into NK cells in vitro in the presence of various cytokines [interleukin (IL)-2, IL-7, IL-15, IL-21, stem cell factor (SCF), and fms-like tyrosine kinase-3 ligand (FLT3L)]. Adding hydrocortisone (HDC) and stromal cells greatly increases the frequency of c-kit<sup>+</sup> BM cells that give rise to CD2<sup>+</sup>CD8<sup>+</sup> NK cells. Also, intracellular levels of perforin, granzyme B, and NKG2D were determined by RT-PCR and western blotting analysis. It was found that of perforin, granzyme B, and NKG2D levels significantly were increased in cytokine-stimulated c-kit<sup>+</sup> BM cells than those of controls. And, we compared the ability of the cytotoxicity of CD2<sup>+</sup>CD8<sup>+</sup> NK cells differentiated by cytokines from c-kit<sup>+</sup> BM cells against K562 target cells for 28 days. Cytokines-induced NK cells as effector cells were incubated with K562 cells as target in a ratio of 100:1 for 4 h once a week. In results, CD2<sup>+</sup>CD8<sup>+</sup> NK cells induced by cytokines and stromal cells showed a significantly increased cytotoxicity 21 days later. Whereas, our results indicated that c-kit<sup>+</sup> BM cells not pretreated with cytokines have lower levels of cytotoxicity. Taken together, this study suggests that cytokines-induced NK cells from porcine c-kit<sup>+</sup> BM cells may be used as adoptive transfer therapy if the known obstacles to xenografting (e.g. immune and non-immune problems) were overcome in the future.
Lim, Dong Kyu,Choi, Myoung Sung Techno-Press 2020 Advances in concrete construction Vol.10 No.5
This study aimed to develop reference materials (RMs) for mortar that can simulate the initial flow characteristics with constant quality over a long period. Through the previous research on the development of RMs for cement paste, the combination of limestone, glycerol, and water was used as the basic matrix for developing RMs for mortar in this study. In addition, glass beads of three particle sizes (0.5, 1.0, and 2.0 mm) and ISO standard sand were selected as tentative candidates to derive fine aggregate substitutes. The mixture of glass beads could simulate the initial flow characteristics of mortar, but under the same mixing ratio, replicates showed an unstable tendency to indicate inconsistent values due to the generation of electrostatic properties between materials and equipment. On the other hand, the mixture using ISO standard Sand not only simulates the constant flow characteristics for a long period of time, but also shows stable results with little error in replicates. Therefore, limestone, glycerol, ISO standard sand, and water were finally determined as components that met the required properties of RMs for mortar. The effect of each component on the flow characteristics of RMs was analyzed. It was found that glycerol increased the cohesion between the particles of standard sand, resulting in a constant increase both in the plastic viscosity and yield stress. Both limestone and standard sand had a dominant effect on the yield stress. The relationships between various mortar mixing ratios and the corresponding mixing ratios of RMs were established. In addition, the results of the verification experiment showed that the rheological properties of the RMs obtained through the relationships correlated with various water/cement ratios and the fine aggregate volume fractions of mortar obtained with same manner. In other words, the RMs for mortar developed in this study can be used as standard samples because they can simulate the initial flow characteristics of mortar of various mixing ratios for a long period without any chemical changes.
Lim, Dong-Kyu,Choi, Myoung-Sung Techno-Press 2022 Advances in concrete construction Vol.14 No.2
Filling materials poured into precast member joint are subjected to restraint stress by the precast member and joint reinforcement. The induced stress will likely cause cracks at early ages and performance degradation of the entire structure. To prevent these issues and design reasonable joints, it is very important to analyze and evaluate the restrained shrinkage cracks of filling materials at various restraint conditions. In this study, a new time zero-that defines the shrinkage development time of a filling material-is proposed to calculate the accurate amount of shrinkage. The tensile stresses and strengths at different ages were compared through the ring test (AASHTO PP34) to evaluate the crack potential of the restrained filling materials at various restraint conditions. The mixture which contained an expansive additive and a shrinkage reducing agent exhibited high resistance to shrinkage cracking owing to the high-drying shrinkage compensation effect. The high-performance, fiber-reinforced cement composite, and ultra-high-performance, fiber-reinforced cement composite yielded very high resistance to shrinkage and cracking owing to the pull-out property of steel fibers. To this end, multiple nonlinear regression analyses were conducted based on the test results. Accordingly, a modified tensile stress equation that considered both the geometric shape of the specimen and the intrinsic properties of the material is proposed.
Generation of $CD2^+CD8^+$ NK Cells from c-$Kit^+$ Bone Marrow Cells in Porcine
Lim, Kyu-Hee,Han, Ji-Hui,Roh, Yoon-Seok,Kim, Bum-Seok,Kwon, Jung-Kee,You, Myoung-Jo,Han, Ho-Jae,Ejaz, Sohail,Kang, Chang-Won,Kim, Jong-Hoon The Korean Society of Pharmacology 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.3
Natural killer (NK) cells provide one of the initial barriers of cellular host defense against pathogens, in particular intracellular pathogens. Because bone marrow-derived hematopoietic stem cells (HSCs), lymphoid protenitors, can give rise to NK cells, NK ontogeny has been considered to be exclusively lymphoid. Here, we show that porcine c-$kit^+$ bone marrow cells (c-$kit^+$ BM cells) develop into NK cells in vitro in the presence of various cytokines [interleukin (IL)-2, IL-7, IL-15, IL-21, stem cell factor (SCF), and fms-like tyrosine kinase-3 ligand (FLT3L)]. Adding hydrocortisone (HDC) and stromal cells greatly increases the frequency of c-$kit^+$ BM cells that give rise to $CD2^+CD8^+$ NK cells. Also, intracellular levels of perforin, granzyme B, and NKG2D were determined by RT-PCR and western blotting analysis. It was found that of perforin, granzyme B, and NKG2D levels significantly were increased in cytokine-stimulated c-$kit^+$ BM cells than those of controls. And, we compared the ability of the cytotoxicity of $CD2^+CD8^+$ NK cells differentiated by cytokines from c-$kit^+$ BM cells against K562 target cells for 28 days. Cytokines-induced NK cells as effector cells were incubated with K562 cells as target in a ratio of 100 : 1 for 4 h once a week. In results, $CD2^+CD8^+$ NK cells induced by cytokines and stromal cells showed a significantly increased cytotoxicity 21 days later. Whereas, our results indicated that c-$kit^+$ BM cells not pretreated with cytokines have lower levels of cytotoxicity. Taken together, this study suggests that cytokines-induced NK cells from porcine c-$kit^+$ BM cells may be used as adoptive transfer therapy if the known obstacles to xenografting (e.g. immune and non-immune problems) were overcome in the future.
구속응력을 받는 프리팹 연결부 채움재료의 수축 및 균열 특성 평가
임동규 ( Lim Dong-kyu ),최명성 ( Choi Myoung-sung ) 한국구조물진단유지관리공학회 2021 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.25 No.2
본 연구는 프리팹 공법의 각 구조 부재들이 일체 거동 하기 위해 연결부에 타설되는 채움재료의 수축 및 균열 특성에 관한 것이다. 프리팹 연결부에 타설된 채움재료는 기존 콘크리트 및 연결부 철근에 의해 구속응력이 발생하며, 재령 초기 마이크로 혹은 관통균열에 의한 구조성능 저하가 발생할 수 있다. 따라서, 구속조건을 가장 유사하게 모사할 수 있는 링테스트를 통해 다양한 채움재료의 수축 및 균열 특성을 분석하였고, 재료에 발생하는 인장응력을 최대한 정확하게 판단하기 위해 재료에 구속응력이 최초로 발현되는 시점을 제안하였다.