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Wang Ling,Bai Xinyang,Qiao Yuanjinzi,Si Lili,Yu Zidi,Ni Chenyang,Li Tianjiao,Guo Chengjin,Xiao Kai 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.4
The members of the microRNA (miRNA) family exert essential roles in modulating plant growth and development as well as responses to diverse stresses, through negatively regulating their target genes at posttranscriptional or translational levels. In this study, we characterized taemiR9674a, a miRNA member in T. aestivum, in mediating plant responses to drought and salt stresses. Seven genes in total were predicted to act as the targets of tae-miR9674a via modulation of transcript cleavage. The transcripts of tae-miR9674a in roots and leaves were response to both stresses of drought and salt, displaying to be gradually upregulated following the progression of a 27-h regime of above stress treatments. The transgenic tobacco lines of tae-miR9674a exhibited modified growth traits under drought and salt treatments. Of these, the line with miRNA overexpression (i.e., Sen 1) improved drastically on plant biomass, leaf area, and root length, whereas that with its knockdown expression (Anti 1) significantly alleviated on above growth traits compared with wild type. The modified stress responses of tae-miR9674a were shown to be closely associated with the role of miRNA in regulating a suite of physiological parameters, of which Sen 1 displayed improved osmotic stress defensive-related traits, such as fastened stomata closing rate, increased leaf water retention capacity, enhanced osmolytes contents, and elevated antioxidant enzyme (AE) activities. The expression of NtP5CS1 involving proline biosynthesis and NtFeSOD, NtCAT1 and NtPOD4, the AE genes involved in modulating ROS homeostasis, was upregulated in Sen 1 upon drought and salt stresses, suggesting their involvement in miRNA-mediated plant drought and salt responses. Transcriptome analysis indicated that tae-miR9674a leads to modified expression of quantities of genes that functionally associate with GO terms “biological process”, “cellular component”, and “molecular function”, which are overrepresented by the biochemical pathways of phytohormones (i.e., ethylene and jasmonic acid), salt response, salt/drought osmotic stress response through abscisic acid-dependent pathway and reactive oxygen species (ROS) homeostasis. Our investigation suggested that tae-miR9674a is an essential mediator in plant osmotic stress tolerance by positively regulating osmotic stress acclimation, cellular ROS homeostasis, and related defensive processes.
Transcriptome reveals genes involving in black skin color formation of ducks
Lei Wang,Hehe Liu,Bo Hu,Jiwei Hu,Hengyong Xu,Hua He,Chunchun Han,Bo Kang,Lili Bai,Rongping Zhang,Jiwen Wang,Shengqiang Hu,Liang Li 한국유전학회 2021 Genes & Genomics Vol.43 No.2
Background Skin color is colorful for birds, which has been reported to be associated with multi-biological functions, such as crypsis, camoufage, social signaling and mate choice, but little is known about its underlying molecular mechanism. Objective Studies on the major genes afecting the black skin color of ducks. Methods For this purpose, Silver ammonia staining and RNA-seq analysis were carried out to identify the diferences in tissue morphology and gene expressions between black and yellow skin ducks. Results The silver ammonia dyes slice results showed that in the development of black duck, the content of melanin in black skin gradually increased and then decreased, and the content of melanin in yellow and black skin was signifcantly diferent. Through transcriptome, a total of 102 and 84 diferentially expressed genes (DEGs) were identifed in beak skin and web skin, respectively. These DEGs were enriched in melanin biosynthesis and play a critical role in melanogenesis pathway. Co-expression analysis showed that EDNRB2 was the only gene associated with black skin color in DEGs, which was also consistent with qRT-PCR. Conclusions The melanin synthesis pathway dominated by EDNRB2 up-regulated the amount of melanin synthesis, leading to the formation of black skin in ducks.
Aerodynamic Design of the Supersonic Aircraft Wing-Shape and Wing-Twist Optimization
Li Li,Junqiang Bai,Tongbiao Guo,Xiaolong He,Ziyuan Fu 한국항공우주학회 2018 International Journal of Aeronautical and Space Sc Vol.19 No.2
This paper builds an aerodynamic optimization design system by coupling the free form deformation parameterization technique,thedynamicmeshtechniquebasedoninversedistanceweightinginterpolationmethodandparticleswarmoptimization algorithm,Krigingsurrogatemodel,withthecomputationalfluiddynamicssolverbasedonReynolds-averagedNavier–Stokes equations. And it carries out aerodynamic optimization designs with a supersonic transport aircraft configuration. The wing shape is parameterized by defining 60 design variables and the wing twist is by four design variables. Meanwhile, the wing thickness constraints are considered. Afterwards, a single-point optimization design aiming at minimum of the supersonic cruise drag is conducted, and in order to consider the transonic cruise condition, a multipoint aerodynamic optimization design aiming at minimum of a weighted drag for the supersonic cruise drag and transonic cruise drag is performed. The analyses of optimization results illustrate the specific drag reduction, the characteristics of pressure distributions for both the supersonic cruise condition and transonic cruise condition, and how the shock wave region and strength have improved on thesupersoniccruisecondition.Theoptimizationdesignthispaperpresentsrealizingthedragreductionisquitepracticaland effective, which is instructive in supersonic aircraft design.