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Advances in CRISPR-Cas systems for RNA targeting, tracking and editing
Wang, Fei,Wang, Lianrong,Zou, Xuan,Duan, Suling,Li, Zhiqiang,Deng, Zixin,Luo, Jie,Lee, Sang Yup,Chen, Shi Elsevier 2019 BIOTECHNOLOGY ADVANCES Vol.37 No.5
<P><B>Abstract</B></P> <P>Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) systems, especially type II (Cas9) systems, have been widely used in gene/genome targeting. Modifications of Cas9 enable these systems to become platforms for precise DNA manipulations. However, the utilization of CRISPR-Cas systems in RNA targeting remains preliminary. The discovery of type VI CRISPR-Cas systems (Cas13) shed light on RNA-guided RNA targeting. Cas13d, the smallest Cas13 protein, with a length of only ~930 amino acids, is a promising platform for RNA targeting compatible with viral delivery systems. Much effort has also been made to develop Cas9, Cas13a and Cas13b applications for RNA-guided RNA targeting. The discovery of new RNA-targeting CRISPR-Cas systems as well as the development of RNA-targeting platforms with Cas9 and Cas13 will promote RNA-targeting technology substantially. Here, we review new advances in RNA-targeting CRISPR-Cas systems as well as advances in applications of these systems in RNA targeting, tracking and editing. We also compare these Cas protein-based technologies with traditional technologies for RNA targeting, tracking and editing. Finally, we discuss remaining questions and prospects for the future.</P> <P><B>Highlights</B></P> <P> <UL> <LI> RNA targeting and editing are becoming increasingly important </LI> <LI> CRISPR-Cas systems are advancing for RNA targeting, tracking and editing </LI> <LI> The type VI CRISPR-Cas systems are useful for RNA-guided RNA targeting </LI> <LI> Use of Cas9 and Cas13 will advance RNA-targeting technologies </LI> </UL> </P>
Wang, Kai,Wang, Yin-Yan,Ma, Jun,Wang, Jiang-Fei,Li, Shao-Wu,Jiang, Tao,Dai, Jian-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24
Several molecular markers have been proposed as predictors of outcome in patients with glioblastomas. We investigated the prognostic significance of $O^6$-methylguanine-DNA methyltransferase (MGMT) promoter methylation and TP53 mutation status dependent on isocitrate dehydrogenase 1 (IDH1) mutation in glioblastoma patients. A cohort of 78 patients with histologically confirmed glioblastomas treated with radiation therapy and chemotherapy were reviewed retrospectively. We evaluated the prognostic value of MGMT promoter methylation and TP53 mutation status with regard to progression-free survival (PFS) and overall survival (OS). It was revealed that mutations in IDH1, promoter methylation of MGMT, TP53 mutation, age, Karnofsky performance status (KFS), and extension of resection were independent prognostic factors. In patients with an IDH1 mutation, those with an MGMT methylation were associated with longer PFS (p=0.016) and OS (p=0.013). Nevertheless, the presence of TP53 mutation could stratify the PFS and OS of patients with IDH1 wild type (p=0.003 and 0.029 respectively, log-rank). The MGMT promoter methylation and TP53 mutation were associated with a favorable outcome of patients with and without mutant IDH1, respectively. The results indicate that glioblastomas with MGMT methylation or TP53 mutations have improved survival that may be influenced by IDH1 mutation status.
( Fei-meng Zheng ),( Wang-bing Chen ),( Tao Qin ),( Li-na Lv ),( Bi Feng ),( Yan-ling Lu ),( Zuo-quan Li ),( Xiao-chao Wang ),( Li-ju Tao ),( Hong-wen Li ),( Shu-you Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2019 BMB Reports Vol.52 No.9
Lymphoma is one of the most curable types of cancer. However, drug resistance is the main challenge faced in lymphoma treatment. Peroxisomal acyl-CoA oxidase 1 (ACOX1) is the rate-limiting enzyme in fatty acid β-oxidation. Deregulation of ACOX1 has been linked to peroxisomal disorders and carcinogenesis in the liver. Currently, there is no information about the function of ACOX1 in lymphoma. In this study, we found that upregulation of ACOX1 promoted proliferation in lymphoma cells, while downregulation of ACOX1 inhibited proliferation and induced apoptosis. Additionally, overexpression of ACOX1 increased resistance to doxorubicin, while suppression of ACOX1 expression markedly potentiated doxorubicin-induced apoptosis. Interestingly, downregulation of ACOX1 promoted mitochondrial location of Bad, reduced mitochondrial membrane potential and provoked apoptosis by activating caspase-9 and caspase-3 related apoptotic pathway. Overexpression of ACOX1 alleviated doxorubicin-induced activation of caspase-9 and caspase-3 and decrease of mitochondrial membrane potential. Importantly, downregulation of ACOX1 increased p73, but not p53, expression. p73 expression was critical for apoptosis induction induced by ACOX1 downregulation. Also, overexpression of ACOX1 significantly reduced stability of p73 protein thereby reducing p73 expression. Thus, our study indicated that suppression of ACOX1 could be a novel and effective approach for treatment of lymphoma. [BMB Reports 2019; 52(9): 566-571]
Wang Chunxiao,Wang Yu,Liu Yu,Li Peng,Zhang Xiantang,Wang Fei 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1
The Vortex-Induced Vibration (VIV) test system on deepwater riser based on Bare Fiber Bragg Grating (BFBG) sensor technology was designed. Meanwhile, a riser VIV response numerical model was established based on the work-energy principle. The results show that the first-order vibration frequency dominates the vibration of the riser, and as the velocity increases, the dominant frequency of the riser gradually increases under the effect of different top tensions. At the same velocity, as the top tension increases step by step, the dominant frequency and fatigue damage at the same position along the axial length of the riser both gradually decreases. The model test and numerical simulation show a relatively consistent change, maintaining a high degree of agreement. The process control system based on BFBG of model test has excellent performance, and FBG sensors have great advantages in VIV test of a vertical riser in water.
Fibulin2: a negative regulator of BMSC osteogenic differentiation in infected bone fracture healing
Li Shi-Dan,Xing Wei,Wang Shao-Chuan,Li You-Bin,Jiang Hao,Zheng Han-Xuan,Li Xiao-Ming,Yang Jing,Guo De-Bin,Xie Xiao-Yu,Jiang Ren-Qing,Fan Chao,Li Lei,Xu Xiang,Fei Jun 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Bone fracture remains a common occurrence, with a population-weighted incidence of approximately 3.21 per 1000. In addition, approximately 2% to 50% of patients with skeletal fractures will develop an infection, one of the causes of disordered bone healing. Dysfunction of bone marrow mesenchymal stem cells (BMSCs) plays a key role in disordered bone repair. However, the specific mechanisms underlying BMSC dysfunction caused by bone infection are largely unknown. In this study, we discovered that Fibulin2 expression was upregulated in infected bone tissues and that BMSCs were the source of infection-induced Fibulin2. Importantly, Fibulin2 knockout accelerated mineralized bone formation during skeletal development and inhibited inflammatory bone resorption. We demonstrated that Fibulin2 suppressed BMSC osteogenic differentiation by binding to Notch2 and inactivating the Notch2 signaling pathway. Moreover, Fibulin2 knockdown restored Notch2 pathway activation and promoted BMSC osteogenesis; these outcomes were abolished by DAPT, a Notch inhibitor. Furthermore, transplanted Fibulin2 knockdown BMSCs displayed better bone repair potential in vivo. Altogether, Fibulin2 is a negative regulator of BMSC osteogenic differentiation that inhibits osteogenesis by inactivating the Notch2 signaling pathway in infected bone.
Controlling Ejection State of a Pneumatic Micro-droplet Generator Through Machine Vision Methods
Fei Wang,Yiwei Wang,Weijie Bao,Hui Zhang,Jiangeng Li,Zhihai Wang 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.4
Pneumatic micro-droplet ejection is a printing technique that has potential applications in many fi elds, especially in thefi eld of bio-printing. The ejection is via a solenoid valve being briefl y turned on, so that high pressure gas enters the liquidreservoir, forming a gas pressure pulse, forcing the liquid out through a tiny nozzle to form a micro-droplet. For bio-printingapplications, the bio-inks are typically non-standard. The diffi culties are not only that the initial working parameters arediffi cult to set, but also the working conditions change over time in many cases. In order to maintain a stable single-dropejection state, a machine vision based ejection monitoring was designed to obtain the number, positions and sizes of thedroplets for each ejection, and a feedback control is realized by adjusting the “ON” time of the solenoid valve or the gaspressure at the front end of the solenoid valve.
Fei-Fei Wang,Yan-Li Ma,Lan Xu,Ping Wang,Yan Zhang 한국섬유공학회 2018 Fibers and polymers Vol.19 No.11
This paper focuses on the semi-blunt puncture behavior of woven fabrics via finite element analysis (FEA) approaches. In these finite element (FE) models, main structure parameters, such as weave structure, warp density as well as friction coefficient, are systematically considered and calculated via FEA. These microstructure geometrical models are builtup with full consideration of interaction between warp and weft yarns. The theoretical results show that plain woven fabric has better puncture resistance compared with 2/1 twill and 5-harness satin fabrics. With the increase of warp density and friction coefficient, the puncture property of woven fabric will be dramatically improved. Finally, an energy consumption method is adopted to analyze the puncture damage process of woven fabric. The simulation results will benefit for the optimum design of woven fabric with excellent puncture performance.
Li, Chunjie,Wang, Guifeng,Li, Fei,Li, Hongmei,Xia, Zhenglong,Liu, Zhan The Korean Institute of Power Electronics 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.4
This paper proposes a fault-tolerant control strategy with finite control set model predictive control (FCS-MPC) based on hierarchical optimization for five-level H-bridge neutral-point-clamped (5L-HNPC) inverter-fed induction motor drives. Fault-tolerant operation is analyzed, and the fault-tolerant control algorithm is improved. Adopting FCS-MPC based on hierarchical optimization, where the voltage is used as the controlled objective, called model predictive voltage control (MPVC), the postfault controller is simplified as a two layer control. The first layer is the voltage jump limit, and the second layer is the voltage following control, which adopts the optimal control strategy to ensure the current following performance and uniqueness of the optimal solution. Finally, simulation and experimental results verify that 5L-HNPC inverter-fed induction motor drives have strong fault tolerant capability and that the FCS-MPVC based on hierarchical optimization is feasible.
Colossal permittivity in (Li + Nb) co-doped Fe2O3 ceramics
Fei Y.M.,Wang Q.Q.,Sun J.,Wang S.T.,Li T.Y.,Wang J.,Wang C.C. 한국물리학회 2020 Current Applied Physics Vol.20 No.7
(Li + Nb) co-doped (Li + Nb)xFe2-xO3 (with x = 0.0005, 0.005, 0.05, and 0.1) ceramics were prepared by solgel method. Their structural, dielectric, humidity, and magnetic properties were investigated. Colossal permittivity (~104) was approached or achieved in all doped samples even with a very small doping level of x = 0.0005. The colossal permittivity behavior is composed of two dielectric relaxations with the low-temperature one being a polaron relaxation due to electrons hopping between Fe3+ and Fe4+ ions and the hightemperature one being a Maxwell-Wagner relaxation caused by humidity-sensing properties.