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Fatigue Strength and Fracture Behaviour of CHS-to-RHS T-Joints Subjected to Out-of-Plane Bending
Li-Chun Bian,Jae-Kyoo Lim,Yon Jig Kim 대한기계학회 2003 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.17 No.2
The fatigue behaviour of six different hollow section T-joints subjected to out-of-plane bending moment was investigated experimentally using scaled steel models. The joints had circular brace members and rectangular chord members. Hot spot stresses and the stress concentration factors (SCFs) were determined experimentally. Fatigue testing was carried out under constant amplitude loading in air. The test results have been statistically evaluated, and show that the experimental SCF values for circular-to-rectangular (CHS-to-RHS) hollow section joints were found to be below those of circular-to-circular (CHS-to-CHS) hollow section joints. The fatigue strength,referred to experimental hot spot stress, was in reasonably good agreement with referred fatigue design codes for tubular joints.<br/>
Fatigue Strength and Fracture Behaviour of CHS-to-RHS T-Joints Subjected to Out-of-Plane Bending
Bian, Li-Chun,Lim, Jae-Kyoo,Kim, Yon-Jig The Korean Society of Mechanical Engineers 2003 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.17 No.2
The fatigue behaviour of six different hollow section T-joints subjected to out-of-plane bending moment was investigated experimentally using scaled steel models. The joints had circular brace members and rectangular chord members. Hot spot stresses and the stress concentration factors. (SCFs) were determined experimentally. Fatigue testing was carried out under constant amplitude loading in air. The test results have been statistically evaluated, and show that the experimental SCF values for circular-to-rectangular (CHS-to-RHS) hollow section joints were found to be below those of circular-to-circular (CHS-to-CHS) hollow section joints. The fatigue strength, referred to experimental hot spot stress, was in reasonably good agreement with referred fatigue design codes for tubular joints.
Azohydromonas aeria sp. nov., isolated from air
Han Xue,Chun-gen Piao,Dan-ran Bian,Min-wei Guo,Yong Li 한국미생물학회 2020 The journal of microbiology Vol.58 No.7
A grey pink colored bacterium, strain t3-1-3T, was isolated from the air at the foot of the Xiangshan Mountain in Beijing, China. The cells are aerobic, Gram-stain-negative, non-sporeforming, motile and coccoid-rod shaped (0.9–1.2 × 1.9–2.1 μm). Strain t3-1-3T was catalase-positive and oxidase-negative and this strain grew at 4–42°C (optimum 28°C), a pH of 4.0–9.0 (optimum pH 7.0) and under 0–2% (w/v) NaCl (optimum 0–1% NaCl). A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain t3-1-3T was closely related to Azohydromonas riparia UCM-11T (97.4% similarity), followed by Azohydromonas australica G1-2T (96.8%) and Azohydromonas ureilytica UCM-80T (96.7%). The genome of strain t3-1-3T contains 6,895 predicted protein-encoding genes, 8 rRNA genes, 62 tRNA genes and one sRNA gene, as well as five potential biosynthetic gene clusters, including clusters of genes coding for non-ribosomal peptide synthetase (NRPS), bacteriocin and arylpolyene and two clusters of genes for terpene. The predominant cellular fatty acids (> 10.0% of the total) in strain t3-1-3T were summed feature 3 (C16:1ω7c and/or C16:1ω6c, 37.8%), summed feature 8 (C18:1ω7c and/or C18:1ω6c, 29.7%) and C16:0 (17.3%). Strain t3-1-3T contained ubiquinone-8 (Q-8) as the predominant respiratory quinone. The polar lipids of strain t3-1-3T comprised phosphatidyl ethanolamine (PE), phosphatidyl glycerol (PG), diphosphatidyl glycerol (DPG), an unidentified glycolipid (GL), an unidentified aminophospholipid (APL), two unidentified phospholipid (PL1-2) and five unidentified lipid (L1-5). The DNA G + C content of the type strain is 70.3%. The broader range of growth temperature, assimilation of malic acid and trisodium citrate, presence of C18:3ω6c and an unidentified glycolipid and absence of C12:0 2-OH and C16:0iso differentiate strain t3-1-3T from related species. Based on the taxonomic data presented in this study, we suggest that strain t3-1-3T represents a novel species within the genus Azohydromonas, for which the name Azohydromonas aeria sp. nov. is proposed. The type strain of Azohydromonas aeria is t3-1-3T (= CFCC 13393T = LMG 30135T).
Jiang, Ping,Yu, Guo-Yu,Zhang, Yong,Xiang, Yang,Hua, Hai-Rong,Bian, Li,Wang, Chun-Yan,Lee, Wen-Hui,Zhang, Yun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6
The role of protease-activated receptors (PARs) in lung tumors is controversial. Although PAR4 is preferentially expressed in human lung tissues, its possible significance in lung cancer has not been defined. The studies reported herein used a combination of clinical observations and molecular methods. Surgically resected lung adenocarcinomas and associated adjacent normal lung tissues were collected and BEAS-2B and NCI-H157 cell lines were grown in tissue culture. PAR4 expression was evaluated by RT-PCR, RT-qPCR, Western blotting and immunohistochemistry analysis. The results showed that PAR4 mRNA expression was generally decreased in lung adenocarcinoma tissues as compared with matched noncancerous tissues (67.7%) and was associated with poor differentiation (p=0.017) and metastasis (p=0.04). Western blotting and immunohistochemical analysis also showed that PAR4 protein levels were mostly decreased in lung adenocarcinoma tissues (61.3%), and were also associated with poor differentiation (p=0.035) and clinical stage (p=0.027). Moreover, PAR4 expression was decreased in NCI-H157 cells as compared with BEAS-2B cells. In conclusion, PAR4 expression is significantly decreased in lung adenocarcinoma, and down-regulation of PAR4 is associated with a more clinically aggressive phenotype. PAR4 may acts as a tumor suppressor in lung adenocarcinoma.