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Li Yuchan,Zhao Jun,Chen Hua,Yu Xia,Li Hui,Zhang Yu,Feng Liang,Wu Zhe,Xie Wenlin,Hou Dabin,Yu Ma 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.2
Bupleurum chinense DC is an important medicinal plant with many active ingredients that are used for the treatment of different types of diseases and valued in pharmaceutical markets. In vitro shoot regeneration can efficiently contribute to the improvement of B. chinense. In the present study, we investigated the effects of the explant type and plant growth regulators (PGRs) on embryogenic callus induction and plant regeneration in B. chinense. Our investigation demonstrated that 2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 1 mg/L thidiazuron (TDZ) played a major role in promoting callus induction from leaf, hypocotyl and stem 2 explants, whereas the most effective treatment for stem 1 callus formation was Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-D, 0.5 mg/L 6-benzyladenine (BA) and 0.5 mg/L kinetin (Kin). The highest shoot regeneration rate (57.14%) was obtained from hypocotyl-induced calli in MS medium with 0.5 mg/L Kin after 12 weeks of cultivation. This regeneration protocol can be used in large-scale cultivation and may be useful for future genetic modifications of B. chinense.
Measurements of proton beam flux and energy of APEP using foil activation technique
Li Wenlin,Dong Qifan,Jing Hantao,Ou Li,Tan Zhixin,Zhuang Sixuan,Wu Qingbiao 한국원자력학회 2024 Nuclear Engineering and Technology Vol.56 No.1
The activation method of metallic foils is an important technique to measure the flux and energy of proton beams. In this paper, the method was used to measure the CSNS APEP proton flux at seven nominal proton energies ranging from 10 MeV to 70 MeV for beam spot sizes of the 20 mm × 20 mm and 50 mm × 50 mm. The reactions of natTi(p, x)48V, natNi(p, x)57Ni, natCu(p, x)58Co, and 27Al(p, x)24Na were employed to measure the proton beam flux with a range of 107-109 p/cm2/s. Furthermore, we also proposed a method using the activity ratio with a stacked-foil target to determine the energy spread of a Gaussian-like distribution for different nominal proton energies. The optimal combinations of Al, Cu, Ti, Ni, Mo, Fe, Nb, and In foils were adopted for the proton energies. The measured energy spreads for degraded beams of 30 MeV–70 MeV were found to be smaller than 10.00%.
Wenlin Ye,Jin Bao,Jie Lei,Yicheng Huang,Zhihao Li,Peisheng Li,Ying Zhang 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.1
A mesoscopic simulation based on random packing powder bed model was established to study the heat behavior of CP-Tiduring selective laser melting. The characteristics of the molten pool under the interaction of laser and powder, and theinfuence of laser power on the thermal behavior, hydrodynamics and surface morphology evolution of the molten pool werestudied. The results show that with the increase of laser power, the maximum temperature, temperature change rate, lifetimeof molten pool and size are greatly improved. In addition, the characteristics and heat behavior of the molten pool under thedouble track are mainly studied in this study. It is found that the maximum temperature, lifetime, and the length and width ofthe molten pool of the second track are higher than those in the frst, and with the increase of laser power, the length widthratio of the second track in molten pool becomes larger
Thermal Model and Numerical Simulation of Selective Laser Alloying Process of Elemental Ti and B
Wenlin Ye,Yuan Tian,Dan Zhou,Peisheng Li,Jie Lei,Ying Zhang 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.8
TiB2 has excellent performances in terms of wear resistance and corrosion resistance, mechanical strength, high elasticmodulus and high compressive strength, hardness, but its application is still limited due to its poor manufacturability. It isexpected to provide a transforming technology for its application by implementing additive manufacture of TiB2. The maincontent of this paper is to propose an innovative technology, selective laser alloying (SLA), using elemental powders ofTitanium and Boron provides a transforming technology for the application of TiB2by enabling its 3D forming from a puresimulation perspective. In this paper, a model of the in-situ reaction process is proposed. By considering the concentrationdistribution of Ti, B and TiB2,the effects of chemical reaction rate and laser power on the evolution of the free solid–liquidinterface and species transport are discussed. The laser energy consumed in the SLA process and the energy released bythe chemical reaction were theoretically derived. It showed that the heat released from the exothermic reaction betweenTitanium and Boron is an important energy source which can reduce the energy input and help improve the manufacturingefficiency of the SLA process; the chemical reaction rate has a significant influence on the concentration distribution of Ti,B and TiB2;the volume and existence time of molten pool and the heat source of reaction strongly relies on the chemicalreaction rate and the laser power.
Segmentation of Leaf Images Based on the Active Contours
Wu Peng,Li Wenlin,Song Wenlong 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.6
Leaves contain important genetic information which can be used as a basis for the identification of plants. As a first step of modeling virtual three-dimensional plant, how to extract visual characteristic information form leaf images has great significance. We propose an optimized C-V model in this paper, which can detect objects in homogeneous regions of given leaf images and speed up running time. The new method combines local information with global information and optimizes the defect that SDF needs to be reconstructed partially so that the energy function is improved. Experimental results show that our algorithm can stop active contours on the correct boundary, get accurate image segmentation, and the speed is more than 1.5 times faster to C-V model.
Harris Scale Invariant Corner Detection Algorithm Based on the Significant Region
Wu Peng,Xu Hongling,Li Wenlin,Song Wenlong 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.3
The traditional Harris corner detection algorithm is sensitive to scale change, corners detected throughout the entire image under complex background, thus extracting more false corners, lead to the follow-up of large amount of calculation and a high rate of error matching. To solve this problem, this paper proposes an optimized Harris corner detection algorithm. First, a significant region detection method is used to extract the target area, and take closing operation for the result figure, can effectively achieve target and background segmentation; second, scale invariant describing methods is applied to Harris algorithm, at the same time, combined with the non-maximum suppression methods to extract corners, get more right corners. Through experiment contrasts, the algorithm used in this paper can be improved more corner detection performance.
Zhao, Jiagang,Sun, Woong,Cho, Hyo Min,Ouyang, Hong,Li, Wenlin,Lin, Ying,Do, Jiun,Zhang, Liangfang,Ding, Sheng,Liu, Yizhi,Lu, Paul,Zhang, Kang American Society for Biochemistry and Molecular Bi 2013 The Journal of biological chemistry Vol.288 No.1
<P>Spinal cord injury (SCI) results in devastating motor and sensory deficits secondary to disrupted neuronal circuits and poor regenerative potential. Efforts to promote regeneration through cell extrinsic and intrinsic manipulations have met with limited success. Stem cells represent an as yet unrealized therapy in SCI. Recently, we identified novel culture methods to induce and maintain primitive neural stem cells (pNSCs) from human embryonic stem cells. We tested whether transplanted human pNSCs can integrate into the CNS of the developing chick neural tube and injured adult rat spinal cord. Following injection of pNSCs into the developing chick CNS, pNSCs integrated into the dorsal aspects of the neural tube, forming cell clusters that spontaneously differentiated into neurons. Furthermore, following transplantation of pNSCs into the lesioned rat spinal cord, grafted pNSCs survived, differentiated into neurons, and extended long distance axons through the scar tissue at the graft-host interface and into the host spinal cord to form terminal-like structures near host spinal neurons. Together, these findings suggest that pNSCs derived from human embryonic stem cells differentiate into neuronal cell types with the potential to extend axons that associate with circuits of the CNS and, more importantly, provide new insights into CNS integration and axonal regeneration, offering hope for repair in SCI.</P>
Truncated Adenomatous Polyposis Coli Mutation Induces Asef-Activated Golgi Fragmentation
Kim, Sang Bum,Zhang, Lu,Yoon, Jimok,Lee, Jeon,Min, Jaewon,Li, Wenlin,Grishin, Nick V.,Moon, Young-Ah,Wright, Woodring E.,Shay, Jerry W. American Society for Microbiology 2018 Molecular and cellular biology Vol.38 No.17
<P>Adenomatous polyposis coli (APC) is a key molecule to maintain cellular homeostasis in colonic epithelium by regulating cell-cell adhesion, cell polarity, and cell migration through activating the APC-stimulated guanine nucleotide-exchange factor (Asef). The APC-activated Asef stimulates the small GTPase, which leads to decreased cell-cell adherence and cell polarity, and enhanced cell migration. In colorectal cancers, while truncated APC constitutively activates Asef and promotes cancer initiation and progression, regulation of Asef by full-length APC is still unclear. Here, we report the autoinhibition mechanism of full-length APC. We found that the armadillo repeats in full-length APC interact with the APC residues 1362 to 1540 (APC-2,3 repeats), and this interaction competes off and inhibits Asef. Deletion of APC-2,3 repeats permits Asef interactions leading to downstream signaling events, including the induction of Golgi fragmentation through the activation of the Asef-ROCK-MLC2. Truncated APC also disrupts protein trafficking and cholesterol homeostasis by inhibition of SREBP2 activity in a Golgi fragmentation-dependent manner. Our study thus uncovers the autoinhibition mechanism of full-length APC and a novel gain of function of truncated APC in regulating Golgi structure, as well as cholesterol homeostasis, which provides a potential target for pharmaceutical intervention against colon cancers.</P>