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A fast, fully automated cell segmentation algorithm for high-throughput and high-content screening
Fenistein, D.,Lenseigne, B.,Christophe, T.,Brodin, P.,Genovesio, A. Wiley Subscription Services, Inc., A Wiley Company 2008 Cytometry. the journal of the International Societ Vol.a73 No.10
<P>High-throughput, high-content screening (HT-HCS) of large compound libraries for drug discovery imposes new constraints on image analysis algorithms. Time and robustness are paramount while accuracy is intrinsically statistical. In this article, a fast and fully automated algorithm for cell segmentation is proposed. The algorithm is based on a strong attachment to the data that provide robustness and have been validated on the HT-HCS of large compound libraries and different biological assays. We present the algorithm and its performance, a description of its advantages and limitations, and a discussion of its range of application. © 2008 International Society for Advancement of Cytometry</P>