在來山羊 수컷의 發育 및 屠體成績

朴忠生,李吉旺,李用斌 慶尙大學校 1972 論文集 Vol.11 No.-

生後 1개월령부터 8개월령 까지의 在來山羊 수컷 38두의 發育 및 屠體調査를 하였다. 生體重은 1개월령에 4.2kg이었고, 8개월령에는 14.5kg이었다. 屠體率은 37.2∼43.4%였으며, 頭重率은 4개월령 이후에 有意的으로 감소했으며, 生體重에 대한 第一胃容積의 比는 6개월령 까지 증가한 후 점차 감소되었다. 7∼8개월령, 山羊 10頭의 精肉率은 29.8%이었으며 兩側 背長筋 斷面績은 7.92㎠이었다. 屠體重, 頭重 및 第一胃容積과 生體重 間에는 高度의 有意的인 正相關이 있었다. The body growth and some carcass data of 38 male Korean native goats from 1 to 8 months of age have been studied. The body weight increased from 4.2kg at 1 month of 14.5kg at 8 months of age. The dressing % ranged from 37.2 to 43.4%. The head % decreased significantly(P<0.05) after 4 months of age. The rumen capacity per kg body weight increased significantly (P<0.05) to 6 moths of age and decreased thereafter. The lean % and loin-eye muscle area of both sides in goats from 7 to 8 months of age averaged 29.8% and 7.92㎠, respectively. There was a significant (P<0.01) correlation between body weight and carcass weight, head weight or rumen capacity.

제주재래마 특이 표지인자 개발

신택순,김선구,강한석,이길왕,조병욱,전해열 密陽産業大學校 農業技術開發硏究所 1998 農業技術開發硏究所報 Vol.2 No.1

본 연구는 제주재래마의 유전적 특성파악과 이를 통한 타품종과의 유전적 특이성을 가지는 DNA 표지인자를 개발하고자 PCR-RAPD기법을 이용하여 제주재래마와 더러브렛, 가고시마의 3품종에서의 다형 band에 대한 특성파악을 실시한 결과이다. 각 품종별로 10두의 혈액을 혼합한 후 분석하였고 200개의 random primer들 중 PCR증폭양상의 재현성을 기준으로 70개의 primer를 선별하였다. 또한 선별된 primer들중 다형성을 9개의 primer를 이용하여 개체별 DNA분석을 실시하였으며 9개의 prkmer에 의해 나타난 15개의 band는 제주재래마 특이 band, 4개는 더러브렛종에 그리고 4개는 가지고시마 재래마에 품종특이 RAPD band가 나타났다. 따라서 RAPD기법에 의해 품종간의 차이를 보이는 표지인자의 개발이 가능할 뿐 아니라 품종간의 특성차이를 보이는 유전인자 개발과 특성파악이 용이한 것으로 평가되었다. This study was carried out using PCR-RAPD technique to estimate genetic characteristics of Cheju Horse (Jorangmal) and to develop the DNA marker for bread identification in horse. Each three horse breeds was represented by pooled DNA from 10 individuals and tested for 200 different random primers for their reproducible PCR pattern. From them 70 primer were selected and 9 primers were used to detect the difference between breeds at the individual level. Fifteen highly polymorphic bands were observed between breeds using 9 primers . Additionally, the 15 most polymorphic bands showed great difference between breeds in their appearing frequencies, implicating their usefulness in the breed discrimination and in the characterization of genetic structure. Therefore, the polymorphism of RAPD could be used in characterizing genomic difference between breeds and some genetic mechanism that give rise to breed disctimination in horse.

Transforming Growth Factor- Increases the Yield of Functional Dopaminergic Neurons from in vitro Differentiated Human Embryonic Stem Cells Induced by Basic Fibroblast Growth Factor

Lee Keum-Sil,Shin Hyun-Ah,Cho Hwang-Yoon,Kim Eun-Young,Lee Young-Jae,Wang Kyu-Chang,Kim Yong-Sik,Lee Hoon-Taek,Chung Kil-Saeng 한국발생생물학회 2003 한국발생생물학회 학술발표대회 Vol.2003 No.1

Embryonic stem (ES) cells proliferate extensively in the undifferentiated state and have the potential to differentiate into a variety of cell types in response to various environmental cues. The generation of functional dopaminergic neurons from ES cells is promising for cell replacement therapy to treat Parkinson's disease. We compared the in vitro differentiation potential of pluripotent human embryonic stem (hES, MB03) cells induced with basic fibroblast growth factor (bFGF) or retinoic acid (RA). Both types of treatment resulted in similar neural cell differentiation patterns at the terminal differentiation stage, specifically, 75% neurons and 11% glial cells. Additionally, treatment of hES cells with brain derived neurotrophic factor (BDNF) or transforming growth factor (TGF)- during the terminal differentiation stage led to significantly increased tyrosine hydroxylase (TH) expression, compared to control (P<0.05). In contrast, no effect was observed on the rate of mature or glutamic acid decarboxylase-positive neurons. Immunostaining and HPLC analyses revealed the higher levels of TH (20.3%) and dopamine in bFGF and TGF- treated hES cells than in RA or BDNF treated hES cells. The results indicate that TGF- may be successfully used in the bFGF induction protocol to yield higher numbers of functional dopaminergic neurons from hES cells.

Transforming Growth Factor-α Increases the Yield of Functional Dopaminergic Neurons from in vitro Differentiated Human Embryonic Stem Cells Induced by Basic Fibroblast Growth Factor

Lee, Keum Sil,Shin, Hyun Ah,Cho, Hwang Yoon,Kim, Eun Young,Lee, Young Jae,Wang, Kyu Chang,Kim, Yong Sik,Lee, Hoon Taek,Chung, Kil Saeng,Park, SePill,Lim, Jin Ho 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

Transforming Growth Factor- Increases the Yield of Functional Dopaminergic Neurons from in vitro Differentiated Human Embryonic Stem Cells Induced by Basic Fibroblast Growth Factor

Lee, Keum-Sil,Shin, Hyun-Ah,Cho, Hwang-Yoon,Kim, Eun-Young,Lee, Young-Jae,Wang, Kyu-Chang,Kim, Yong-Sik,Lee, Hoon-Taek,Chung, Kil-Saeng,Park, Se-Pill 한국수정란이식학회 2003 한국수정란이식학회 학술대회 Vol.2003 No.1

Development of Paternity Testing System Using RAPD

Lee,Kil Wang,Kang,Han Seok,Cho,Byung Wook 密陽産業大學校 農業技術開發硏究所 1999 農業技術開發硏究所報 Vol.3 No.1

본 연구는 임의의 DNA틀 증폭시키는 RAPD를 실하여 태어난 후대들의 정확한 부 ·모를 추정할 수있도록 DNA 수준에서 친자감별 및 개체식별을 할 수 있는 시스템을 개발하여 우수종축의 보존 및 유지개량을 위한 기초자료를 제공하고, 또 DNA fingerprintrin방법보다 DNA 수준에서 친자감별 및 개채실별을 간편하고 빠르게 할 수 있는 새로운 기법으로서 ?? 같은 방사선 동위원자를 사용하지 않고 간편하게 분석할 수 있는 임의의 DNA를 증폭시키는 RAPD를 이용한 친자감별 시스템을 개발 하고자 수행하였다. DNA fingerprinting보다 빠르고 간편한 시스템을 개발하기 위해 300여개의 임의 primer을 이용하여 분석한 결과 5개에서 얻어진 RAPD밴드패턴들이 멘텔유전양식에 따라 자식에게 전달된미 추정되어 RAPD pnarkern 친자감별 및 개체식별의 유음한 분자기술임이 입증되었다. This study was conducted to develop find the paternity testing system using a RAPD. Of the initial 300 random primers, 120 primers produced clear and intense bands following PCR amplification Despite the large number of primers screened, most of the primers generating interpretable amplification profiles showed monomorphic pattern among individuals. Among120 primers five primers were selected to identify the paternity testing.

Analysis of the Factors of Velvet Antler Yields in Sika Deer

Kil-Wang Lee,Byung-Wook Cho 한국생명과학회 2004 한국생명과학회 심포지움 Vol.43 No.-

발광박테리아를 이용한 생물감시장치의 중금속에 대한 독성반응

이길수(Kil-Soo Lee),이승주(Seung-Joo Lee),왕창근(Chang-Keun Wang),최영균(Young-Gyun Choi),홍금용(Keum-Yong Hong) 대한환경공학회 2020 대한환경공학회지 Vol.42 No.2

목적 : 본 연구에서는 발광박테리아(Vibrio fischeri)가 방출한 빛의 강도가 약화되는 것을 측정하는 실시간 생물감시장치에서의 중금속(Zn<SUP>2+</SUP>, Pb<SUP>2+</SUP>, Hg<SUP>2+</SUP>, Cd<SUP>2+</SUP>)에 대한 독성반응을 평가하였다. 방법 : 발광저해도 시험은 4가지 중금속과 중금속이 첨가된 폐수 방류수를 포함하고 있다. 시험 시료에 대한 독성도는 동결 건조된 박테리아(Vibrio fischeri NRRL-B-11177)를 이용하여 생물검정을 수행하였다. 시료의 pH가 나타낼 수 있는 잠재적인 영향을 제거하기 위하여 시험 전에 pH를 7±0.2로 조절하였다. 생물감시장치는 발광된 빛으로부터 2분의 단시간 내에 시료의 독성도를 측정하여 결과를 산출한다. 그 후에 발광저해도 곡선으로부터 EC20과 EC50을 추정하였다. 결과 및 토의 : 중금속 희석액을 대상으로 한 발광저해도 시험의 경우, 각 중금속의 EC20은 Zn(II) < 0.2 mg/L, Hg(II) 0.45 mg/L, Cd(II) 0.58 mg/L 및 Pb(II) 1.95 mg/L로 추정되었고, EC50은 Zn(II) 0.25 mg/L, Hg(II) 0.5~1.0mg/L, Cd(II) 1.38 mg/L 및 Pb(II) 3.76 mg/L로 추정되었다. 중금속의 민감도는 Zn(II) > Hg(II), > Cd(II) > Pb(II) 순서로 나타났다. 중금속이 첨가한 폐수 방류수를 대상으로 한 발광저해도 시험의 경우, 각 중금속을 첨가한 시료의 EC20은 Hg(II) 0.38 mg/L, Zn(II) 0.58 mg/L, Pb(II) 1.45 mg/L 및 Cd(II) 1.95 mg/L로 추정되었고, EC50은 Hg(II) 0.53 mg/L, Zn(II) 1.13 mg/L, Pb(II) 6.44 mg/L 및 Cd(II) 7.82 mg/L로 추정되었다. 중금속이 첨가한 폐수에 대한 민감도는 Hg(II) > Zn(II) > Pb(II) > Cd(II) 순서로 나타났다. 우리나라의 수질오염물질 배출허용기준과 비교해서 Zn(II)의 EC20은 낮은 농도이기 때문에 방류수의 배출허용기준을 만족하는지를 감시하는 것은 적합하다고 판단된다. 그러나 그 외의 중금속의 EC20 수준으로는 단위공정의 제어나 사고로 인한 방류를 감시하는 목적으로 적용하는 것이 바람직하다. 결론 : 본 연구에서는 발광박테리아를 이용한 실시간 생물감시장치로 4가지 중금속과 중금속을 첨가한 시료에 대하여 독성도를 평가하였다. Zn(II)가 첨가된 폐수의 EC20은 0.58 mg/L로 배출허용기준보다 낮은 것으로 나타나 배출허용기준 초과 유무를 판별하는데 적절하다고 판단된다. 실시간 생물감시장치의 운영과 더불어 산업폐수처리시설에 대한 통합적 감시를 위해 물리화학적 분석을 병행할 필요가 있다. Objectives : The purpose of this study is to evaluate the effects of heavy metals (Zn<SUP>2+</SUP>, Pb<SUP>2+</SUP>, Hg<SUP>2+</SUP>, Cd<SUP>2+</SUP>) toxicity in a real-time biosensor which is based on measuring the attenuation of light intensity emitted by Vibrio fischeri. Methods : The inhibition test included four heavy metals and the spiked effluent wastewater. The toxicity of the test samples was assessed by freeze-dried bacteria (Vibrio fischeri NRRL-B-11177). The pH of the samples was adjusted to 7±0.2 before conducting the toxicity tests in oder to eliminate potential pH effects. The results obtained from the real-time biosensor that can be measuring the toxicity of the samples as a function of the emitted light. within a short period time (2 min). And then EC20 and EC50 values was estimated from inhibition curves. Results and Discussion : In case of the inhibition test for dilluents of heavy metals, EC20 values were estimated as < 0.2 mg/L for Zn(II), 0.45 mg/L for Hg(II), 0.58 mg/L for Cd(II) and 1.95 mg/L for Pb(II) and EC50 values were estimated as 0.25 mg/L for Zn(II), 0.5~1.0 mg/L for Hg(II), 1.38 mg/L for Cd(II) and 3.76 mg/L for Pb(II). The sensitivity ranking of heavy metals was in order as Zn(II) > Hg(II), > Cd(II) > Pb(II). In case of the inhibition test for the spiked effluent wastewater, EC20 values were estimated as 0.38 mg/L for Hg(II), 0.58 mg/L for Zn(II), 1.45 mg/L for Pb(II) and 1.95 mg/L for Cd(II) and EC50 values were estimated as 0.53 mg/L for Hg(II), 1.13 mg/L for Zn(II), 6.44 mg/L for Pb(II) and 7.82 mg/L for Cd(II). The sensitivity ranking of the spiked effluent wastewater was in order as Hg(II) > Zn(II) > Pb(II) > Cd(II). Compared to the Emssion Limit Value (ELV) in Korea, the EC20 value of Zn(II) was found to be lower than ELV so that it was suitable for screening of Zn(II) as to meet the criteria of effluent wastewater. However, the other EC20 value of heavy metals can be used to control the unit processes and avoid accidental discharge. Conclusions : The assessment of the toxicity of four heavy metals and spiked samples with them was performed in this study, by real-time biosensor using bioluminescent bacteria. The EC20 value of the spiked sample with Zn(II) has been found to be 0.58 mg/L lower than its ELV so that it was considered as a suitable screening for determining whether or not to exceed the permissible limit value. The use of real-time biosensor is required in combination with physicochemical analyses for an integrated screening of the industrial effluent properties.

단일광자방출전산화단층촬영을 이용한 정신분열증 환자의 국소 뇌혈류 변화에 대한 평가

이요왕 ( Yo Wang Lee ),이경한 ( Kyeng Han Lee ),채정호 ( Jung Ho Chai ),한광수 ( Kwang Su Han ),노왕구 ( Wang Ku Rho ),김원길 ( Won Kil Kim ) 국군의무사령부 1993 대한군진의학학술지 Vol.24 No.1

Acquired Tufted Angioma

왕정길 ( Jeong Kil Wang ),이학중 ( Hak Joong Lee ),왕한영 ( Han Young Wang ),윤혜경 ( Hye Kyoung Yoon ),박필성 ( Pill Sung Park ) 대한피부과학회 1998 대한피부과학회지 Vol.36 No.1

Acquired tufted angioma is a benign, slowly progressive angioma with a typical histological pattern that was first described by Wilson-Jones in 1976. We report a case of acquired tufted angioma in a 19 year old female who had erythematous papules and plaques on the right thigh. Histopathological findings showed multiple capillary lobules in a cannonball arrangement scattered throughout dermis, which was diagnostic of acquired tufted angioma. (Korean J Dermatol 1998;36(1): 164-167)