The Effects of Alpinia katsumadai Extract on Anti-inflammation and Melanogenesis

( Jae Soeb Lee ),( See-hwa Jeong ),( Kang-ii Ko ),( Ki Ho Kim ),( Chom-kyu Chong ),( Soo Nam Park ) 대한화장품학회 1999 대한화장품학회지 Vol.25 No.1

Several solvent extracts from Alpinia katsumadai were prepared and their various anti-inflammatory activities were evaluated. Alpinia katsumadai extract showed high various anti-inflammatory effects among the 8 medicinal plant extracts. Butanol extract from Alpinia katsumadai showed a potent anti-oxidative and free radical scavenging activities. Free radical scavenging effect of butanol fraction of Alpinia katsumadai(lC₅₀ : 5μg/㎖) was higher than butylated hydroxytoluene(IC₅₀ : 50/μg/㎖) and ascorbic acid(IC₅₀ : 22μg/㎖). Alpinia katsumadai butanol fraction exhibited relatively high antioxidative activity(IC₅₀ : 80μg/㎖) compared to ascorbic acid. The inhibitory effect of Alpinia katsumadai ethanol extract on elastase exhibited 10 to 78% at 100 to 1000μg/㎖ concentration ; the IC₅₀ values with 465.7μg/㎖ for porcine pancreatic elastase(PPE) and 481.9μg/㎖ for human leukocyte elastase(HLE), respectively. The Alpinia katsumadai extract inhibited effectively hyaluronidase activity(IC₅₀ : 335μg/㎖), and showed inhibition in vitro on delayed hypersensitivity when it was topically applied. These results suggest that Alpinia katsumadai extract may reduce inflammatory skin trouble. The Alpinia katsumadai extract also showed higher inhibitory effect of melanin biosynthesis on cultured melanoma cell compared to arbutin and kojic acid.

Ra 동위체로 본 하계 동해 남부 연안해역에 출현하는 10℃ 이하 냉수괴의 기원

이재철,문창호,양한섭,김평중 한국수산학회 1994 한국수산과학회지 Vol.27 No.4

하계 한국 동해 남부 연안해역의 4개 단면에서 1991년 9월 2∼8일 사이에 수온관측 및 Ra동위체의 농도를 측정하였다. 수온의 연직분포를 보면, 북쪽 단면 A와 B에서는 강한 계절수온약층이 대체적으로 표층 10∼30 m 사이의 수층에 존재하고, 단면 C에서는 30∼50 m 수층에 수온약층이 나타난다. 단면 D에서는 특이하게 2개의 수온약층이 존재하고 연안쪽 정점에서 바깥쪽 정점으로 갈수록 수온약층의 존재수심은 급격히 깊어지는 것이 특징적이다. 그리고, 수온약층 상부에는 수온 20℃이상의 대마난류표층수가 존재하고, 수온약층 바로 아래 수층에는 수온 12∼17℃의 대마난류중층수가 나타나며, 이 아래 수층에는 기원이 불분명한 10℃이하의 냉수괴가 나타나고, 수심 약 150 m 이심층에는 1℃ 이하의 동해고유수가 존재한다. 9개 정점의 2∼3개 수층에서 채수한 해수의 Ra동위체 농도를 측정한 결과, 대마난류표층수의 특성값은 Ra-228이 225±23 dpm/㎘, Ra-226이 99±6 dpm/㎘이며, Ra-228/Ra-226 방사능의 비는 1.9∼2.6범위였다. 대마난류중층수의 특성값은 Ra-228이 71±12 dpm/㎘로 표층수중 농도의 약 ⅓정도이고, Ra-226은 80±6 dpm/㎘이며, 두 동위체의 비는 0.7∼1.1범위였다. 한편, 수온 2∼6℃범위의 냉수괴는 Ra-228이 59 ±10 dpm/㎘이고, Ra-226은 85±9 dpm/㎘였으며, 두 동위체의 비는 0.6∼0.9범위였다. 65∼120 m 사이의 수층에서 수온 2∼6℃범위를 보이는 냉수괴의 기원을 밝히기 위하여 수온에 대한 용존산소 및 Ra동위체의 농도(혹은 동위체비)의 diagram을 분석하였다. 그 결과, 이 냉수괴는 북한한류수인 것으로 판명되었다. T-O₂ diagram으로는 이 냉수괴의 기원은을 알기 어려웠지만 Ra동위체의 농도를 이용하면 보다 명료하게 그 기원을 알 수 있었다. 그러므로, 동해에서 Ra동위체의 연직분포 측정은 각종 수괴의 혼합확산이나 변질과정을 이해하는 데 유용할 것이다. Radium isotopes were measured together with oceanographical parameters in the southern coastal region of the Korean East Sea during the period of September 2∼8, 1991. In September, there were various water masses vertically distributed in this region due to formation of strongly seasonal thermoclines. These water masses were characterized by activity of radium isotopes as well as water temperature and dissolved oxygen. Among the water masses, Japan Sea Proper Water(JSPW) below 1℃ had the highest Ra-226 activities but the lowest Ra-228 concentrations. However, Tsushima Surface Water (TSW) above 20℃ in water temperature had the highest Ra-228 which decreased sharply with depth. In TSW, Ra-228 activities were in the range of 194∼270 dpm/㎘, which were approximately 10 times higher than JSPW. Activity ratios(A.R.'s) of Ra-228/Ra-226 were 1.9∼2.6 for TSW, 0.7∼1.1 for Tsushima Middle Water(TMW) of 12∼17℃, 0.4∼0.7 for North Korea Cold Water(NKCW) with 1∼7℃ and below 0.2 for JSPW. The Ra-228/Ra-226 ranged from 0.6 to 0.9 in the cold waters of 2∼6℃, which were observed at depth of 65∼120 m in this study area. Radium isotopes provided a useful means of identifying origins of the cold water which occurred annually at intermediate or bottom layers in the southern coastal zone of the Korean East Sea. By plotting radium isotopes against water temperature, it could be observed clearly that the cold waters between 2∼6℃ did not originate from the mixed water of JSPW and TMW but from NKCW.

보호계전기 정정을 위한 편리한 사용자 환경설계

최항섭,이승재 명지대학교 대학원 1997 대학원논문집 Vol.1 No.-

It is differcult to use a computer. The reason is a complex procedure of task. Thus the step is required to make user-interface a man-based design. This paper proposes a design user friendly graphic environment of the protective relay setting.

보호계전기 정정을 위한 편리한 사용자 환경설계

최항섭(Hang-Soeb Choi),이승재(Seung-Jae Lee),강상희(Sang-Hee Kang),최면송(Myeon-Song Choi),안준기(Jon-Gi An),이운희(Oon-Hee Lee) 대한전기학회 1997 대한전기학회 학술대회 논문집 Vol.1997 No.11

Dempster-Shafer 증거이론을 이용한 산업 전력 계통의 지능적 보호 시스템 설계

이승재(Seung-Jae Lee),차민철(Min-Cheul Cha),최항섭(Hang-Soeb Choe),김상태(Sang-Tae Kim),김봉희(Bong-Hee Kim) 대한전기학회 1997 대한전기학회 학술대회 논문집 Vol.1997 No.7

Secondary Metabolite Discovery and Material Development of Bifidobacterium Strains

Mijeong KIM,Jiyeon LEE,Ahreum JUNG,Jun-Tae BAE,Jae Soeb LEE 한국생물공학회 2022 한국생물공학회 학술대회 Vol.2022 No.9

INTERACTION OF ACETOLACTATE SYNTHASE WITH TRIAZOLOPYRIMIDINE INHIBITORS

Sung Ho Kim,Jae Soeb Lee,Sung Keon Namgoong,Soo Ik Chang,Jung Do Choi 생화학분자생물학회 1997 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

PURIFICATION AND FUNCTIONAL AMINO ACID RESIDUES OF ACETOLACTATE SYNTHASE FROM PEA

Yung Soo Shin,Jae Soeb Lee . Jong Gun Chae,Jung Hyu Shin,Jung Do Choi 생화학분자생물학회 1996 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

동해 중부해역에서 ²¹⁰Po과 ²³⁴Th의 순환에 대한 소용돌이의 영향

양한섭,김성수,이재철,YANG, HAN SOEB,KIM, SOUNG SOO,LEE, JAE CHUL 한국해양학회 1995 韓國海洋學會誌 Vol.30 No.4

The vertical profiles of natural ²¹⁰Pb, ²¹⁰Po and ²³⁴Th activities were measured for the upper 100 m of water column at three stations in the middle region of the Korean East Sea during May 1992. And the distribution of these radionuclides was discussed associated with the formation of warm eddy or water mass. The main thermocline was maintained between the depth of 50 and 100 m at the southern station (Sta. A1), and between the depth of 10 to 50 m at the coastal station of Sockcho (Sta. B10). Contrastingly, a main thermocline at Sta. A10, which locates near the center of warm eddy, was observed below 230 m depth. Between 50 and 220 m depth of Sta. A10 is there a relatively homogeneous water mass of 10.1${\pm}$0.5$^{\circ}C$, which is significantly higher in temperature and lower in nutrient than the other two stations. It seems to be due to sinking of the warm surface water in which nutrients were completely consumed. Both ²¹⁰Pb and ²¹⁰Po show the highest concentration at Sta. A1 and the lowest at Sta. B10 among the three stations. Also, the ²¹⁰Pb activity is generally higher in the upper layer than in the lower layer, while ²¹⁰Po activity represents the reversed pattern at all three stations. At Sta. A1 and Sta. B10, the activities of ²¹⁰Po relative to its parent ²¹⁰Pb were deficient in the water column above the main thermocline, but were excess below the thermocline. However, the station near the center of warm eddy(Sta. A10), shows no excess of ²¹⁰Po in the depths below 50 m, although its defficiency is found in the upper layer like the other stations. At Sta. A1 and b10. ²³⁴Th activities are slightly lower in the surface mixed layer than in the deeper region However, at Sta. A10, ²³⁴Th activity in the upper 30 m is higher than below 50 m or in the same depth of the other stations, probably because of the high concentration of particulate matter. The residence time of ²¹⁰Po in the surface mixed layer at Sta. A10 is 0.4 year, much shorter than at the other two stations(about one year). Above 100 m depth, the residence times of ²³⁴Th range from 18 to 30 other two stations(about on year). Above 100 m depth, the residence times of ²³⁴Th range from 18 to 30 days at all stations, without significant regional variation. The percentages of recycled ²¹⁰Po within the thermocline are 39% and 92% at Sta. A1 and Sta. B10, respectively. Much higher value at Sta. B10 may be due to a thin thickness of the mixed layer as well as the slower recycling rate of ²¹⁰Po in the main thermocline.

Triazolopyrimidine계 저해제와 보리 Acetolactate Synthase와의 상호작용

이재섭,장수익,남궁성건,신정휴,최정도,Lee, Jae Soeb,Chang, Soo Ik,Nam Goong, Sung Keon,Shin, Jung Hyu,Choi, Jung Do 대한화학회 1998 대한화학회지 Vol.42 No.3

Acetolactate synthase(ALS)는 박테리아, yeast, 그리고 고등 식물에서 가지를 가진 아미노산 Val, Leu, Ile의 생합성에 공통적으로 관여하는 필수적인 효소이다. 최근에 개발된 sulfonylurea, imidazolinone, triazolopyrimidine, 그리고 pyrimidyl-oxy-benzoate계 제초제들은 구조적으로 상호 유사성이 없음에도 불구하고 모두 ALS를 작용 표적으로 한다. Triazolopyrimidine(TP)계의 새로운 유도체들을 합성하여 보리의 ALS에 대해 저해활성을 측정하였다. 활성을 나타낸 저해제들의 $IC_{50}값은 3.2nM-0.62mM로 몇 개의 유도체는 뛰어난 활성을 보였다. 보리 ALS에 대해 triazolopyrimidine 유도체 TP4의 저해활성은 반응 시간이 증가함에 따라 증가하였고, 혼합형 저해유형을 보여주었다. TP4와 imidazolinone 제초제인 Cadre, 그리고 feedback 저해제인 Leu에 대한 dual inhibition 실험 결과 모두 평행한 kinetic pattern이 얻어져 이들 저해제의 결합 부위가 최소한 부분적으로 중복되는 부분이 있음을 시사했다. ALS의 Tyr의 변형은 TP4에 의한 저해 효과를 감소시키는 반면 Trp과 Cys 변형은 TP4의 결합에 영향을 나타내지 않았다. Acetolactate synthase (ALS) is the common enzyme in the biosynthesis of branched chain amino acids, Val, Leu, and Ile in bacteria, yeast, and higher plants. The enzyme is target site of several classes of structually diverse herbicides, including the sulfonylureas, the imidazolinones, the triazolopyrimidines, and the primidyl-oxy-benzoates. We have synthesized new triazolopyrimidine (TP) derivatives, and determined their inhibitory activities on barley ALS. $lC_{50}$ values for the active compounds were 3.2 nM-0.62 mM, and some of them appeared to be potent inhibitors. The progress curves for inhibition of ALS by TP4, a representative derivative, indicated that the extent of inhibition increased with incubation time. The inhibition of ALS by TP4 showed mixed-type inhibition with respect to pyruvate. Dual inhibition analyses of TP4 versus imidazolinone Cadre and feedback inhibitor Leu suggested that three different classes of inhibitors bind to ALS in a mutually exclusive manner. Chemical modification of tyrosyl residues of ALS decreased sensitivity of ALS to TP4, while modification of tryptophan and cysteine did not affect the sensitivity.