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Lee, Yong-Jin,Lee, Tong-Geon,Jeon, Woong-Bae,Kim, Dae-Yeon,Hong, Min-Jeong,Lee, Man-Bo,Hyun, Jong-Nae,Kim, Mi-Jung,Lee, Mi-Ja,Park, Cheol-Su,Seo, Yong-Weon 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.2
Cereal seed storage proteins are encoded by complex multigene families and their subunit profiles are highly related to end-use qualities. Each fraction of albumin and hordein was extracted and its subunit profile was evaluated in related to malt and grain quality parameters. The purpose of this study was to provide selection criteria for high quality malting barley using grain and malt quality parameters and biochemical-genetic information. Grain and malt quality of 13 local adaptability test (LAT) lines were evaluated for malting process. A total of 16 germplasm accessions of high or low seed storage protein content were also evaluated for biochemical-genetic analysis. The correlation coefficients among quality parameters were analyzed. Several important quality parameters in brewing process showed significant positive or negative correlations. Seed storage protein subunits of albumin and hordein of all tested lines and accessions were evaluated using 12% 1D SDS-PAGE. Scored data of protein subunit's presence or absence was applied to Agglomerative Hierarchical Clustering (AHC) for statistical analysis and showed specific grouping patterns among tested lines. Clustered lines with subunit information were highly related with agricultural performance and grain and malt qualities. Based on the profiles of seed storage protein subunits, association of hordein subunit of 38, 43, and 65 kDa with high malt scored lines was found. The obtained results would provide improved selection criteria for high quality malting barley in the malting barley breeding program.
Lee, Geon-Woong,Bang, Dae-Suk,Cho, Dong-Hwan,Kumar, Satish Korean Carbon Society 2007 Carbon Letters Vol.8 No.4
A method for evaluating bulk sensitive structural characteristics of unpurified, as-purified, and acid treated single walled carbon nanotubes (SWNTs) was described in the present study. The optical spectra of SWNT solutions were well resolved after prolonged sonication and they were correlated to the diameter and the distribution of nanotubes. The acid-treated SWNTs were similar to as-purified SWNTs in terms of catalyst residue, radial breathing mode (RBM) in the Raman spectra, and the first band gap energy of semiconducting tubes in the optical spectra. The solution phase optical spectra were more sensitive to changes in the small diameter and metallic tubes after the acid treatment than were the RBM spectra.
( Yeong Geon Lee ),( Jae Woong Hwang ),( Sang Bum Park ),( Il Seob Shin ),( Soo Kyung Kang ),( Kwang Won Seo ),( Yong Soon Lee ),( Kyung Sun Kang ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4
Therapy using stem cells for the liver fibrosis is a prospective alternative to overcome the insufficiency of transplantable liver donor. Here, we demonstrated xenogeneic human cell therapy for the treatment of rat liver fibrosis without the use of an immunosuppressant. Liver fibrosis was induced by dimethylnitrosamine for 5 weeks in six-week-old male Sprague-Dawley rats. Human umbilical cord blood- and adipose tissue-derived multipotent stem cells were injected intravenously by the tail vein after one week. Blood samples were collected and liver samples were stained with Masson`s trichrome in order to evaluate the amount of fibrosis. After the cell injection, the level of total protein, albumin, alanine transaminase and aspartic acid transaminase was recovered to the similar level of the normal rats. The liver weight per body weight increased after the cell injection. Collagen fiber, near the portal triad and marginal region, was reduced, significantly. Taken together, it is suggested that xenotransplantation of multipotent stem cells might be a candidate for the treatment of liver fibrosis without the use of an immunosuppresant.