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Le Ve Van,Ko So-Ra,Kang Mingyeong,Jeong Seonah,Oh Hee-Mock,Ahn Chi-Yong 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.11
The three Gram-negative, catalase- and oxidase-positive bacterial strains RS43T , HBC28, and HBC61T , were isolated from fresh water and subjected to a polyphasic study. Comparison of 16S rRNA gene sequence initially indicated that strains RS43T , HBC28, and HBC61T were closely related to species of genus Curvibacter and shared the highest sequence similarity of 98.14%, 98.21%, and 98.76%, respectively, with Curvibacter gracilis 7-1T . Phylogenetic analysis based on genome sequences placed all strains within the genus Curvibacter. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the three strains and related type strains supported their recognition as two novel genospecies in the genus Curvibacter. Comparative genomic analysis revealed that the genus possessed an open pangenome. Based on KEGG BlastKOALA analyses, Curvibacter species have the potential to metabolize benzoate, phenylacetate, catechol, and salicylate, indicating their potential use in the elimination of these compounds from the water systems. The results of polyphasic characterization indicated that strain RS43T and HBC61T represent two novel species, for which the name Curvibacter microcysteis sp. nov. (type strain RS43T =KCTC 92793T =LMG 32714T ) and Curvibacter cyanobacteriorum sp. nov. (type strain HBC61T =KCTC 92794T =LMG 32713T ) are proposed.
( Ve Van Le ),( So-ra Ko ),( Sang-ah Lee ),( Mingyeong Kang ),( Hee-mock Oh ),( Chi-yong Ahn ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.5
A Gram-stain-negative, white-coloured, and rod-shaped bacterium, strain DR4-4<sup>T</sup>, was isolated from Daechung Reservoir, Republic of Korea, during Microcystis bloom. Strain DR4-4<sup>T</sup> was most closely related to Caenimonas terrae SGM1-15<sup>T</sup> and Caenimonas koreensis EMB320<sup>T</sup> with 98.1% 16S rRNA gene sequence similarities. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain DR4-4<sup>T</sup> and closely related type strains were below 79.46% and 22.30%, respectively. The genomic DNA G+C content was 67.5%. The major cellular fatty acids (≥10% of the total) were identified as C<sub>16:0</sub>, cyclo C<sub>17:0</sub>, summed feature 3 (C<sub>16:1</sub>ω7c and/or C<sub>16:1</sub>ω6c), and summed feature 8 (C<sub>18:1</sub>ω7c and/or C<sub>18:1</sub>ω6c). Strain DR4-4<sup>T</sup> possessed phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol as the main polar lipids and Q-8 as the respiratory quinone. The polyamine profile was composed of putrescine, cadaverine, and spermidine. The results of polyphasic characterization indicated that the isolated strain DR4-4<sup>T</sup> represents a novel species within the genus Caenimonas, for which the name Caenimonas aquaedulcis sp. nov. is proposed. The type strain is DR4-4<sup>T</sup> (=KCTC 82470<sup>T</sup> =JCM 34453<sup>T</sup>).
Mucilaginibacter aquariorum sp. nov., Isolated from Fresh Water
Le Ve Van,Ko So-Ra,Kang Mingyeong,Oh Hee-Mock,안치용 한국미생물·생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.12
A Gram-stain-negative, rod-shaped bacterial strain, JC4T , was isolated from a freshwater sample and determined the taxonomic position. Initial identification based on 16S rRNA gene sequences revealed that strain JC4T is affiliated to the genus Mucilaginibacter with a sequence similarity of 97.97% to Mucilaginibacter rigui WPCB133T . The average nucleotide identity and digital DNA–DNA hybridization values between strain JC4T and Mucilaginibacter species were estimated below 80.92% and 23.9%, respectively. Strain JC4T contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and iso-C15:0 as predominant cellular fatty acids. The dominant polar lipids were identified as phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, and two unidentified lipids. The respiratory quinone was MK-7. The genomic DNA G+C content of strain JC4T was determined to be 42.44%. The above polyphasic evidences support that strain JC4T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter aquariorum sp. nov. is proposed. The type strain is JC4T (= KCTC 92230T = LMG 32715T ).
Le Ve Van,Ko So-Ra,Oh Hee-Mock,Ahn Chi-Yong 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.12
Microcystis blooms threaten ecosystem function and cause substantial economic losses. Microorganismbased methods, mainly using cyanobactericidal bacteria, are considered one of the most ecologically sound methods to control Microcystis blooms. This study focused on gaining genomic insights into Paucibacter aquatile DH15 that exhibited excellent cyanobactericidal effects against Microcystis. Additionally, a pan-genome analysis of the genus Paucibacter was conducted to enhance our understanding of the ecophysiological significance of this genus. Based on phylogenomic analyses, strain DH15 was classified as a member of the species Paucibacter aquatile. The genome analysis supported that strain DH15 can effectively destroy Microcystis, possibly due to the specific genes involved in the flagellar synthesis, cell wall degradation, and the production of cyanobactericidal compounds. The pan-genome analysis revealed the diversity and adaptability of the genus Paucibacter, highlighting its potential to absorb external genetic elements. Paucibacter species were anticipated to play a vital role in the ecosystem by potentially providing essential nutrients, such as vitamins B7, B12, and heme, to auxotrophic microbial groups. Overall, our findings contribute to understanding the molecular mechanisms underlying the action of cyanobactericidal bacteria against Microcystis and shed light on the ecological significance of the genus Paucibacter.
Complete genome sequence of Bacillus aryabhattai K13 isolated from compost
파틸키소서레쉬바이,이승제,Ve Van Le,전소현,채종찬 한국미생물학회 2017 미생물학회지 Vol.53 No.4
Bacillus aryabhattai K13 was originated from enriched culture of compost using 1% kraft lignin as a sole carbon and energy source. The complete genome of strain K13 consists of one 5.0 Mb chromosome and two circular plasmids with 139 kb and 78 kb, respectively. Analysis of the genome determined in this study may contribute to identify the genes responsible for degradation of lignin.
Growth-promoting Effect of Alginate Oligosaccharides on Rhodobacter sphaeroides
NGUYEN THI MY LE,Ve Van Le,신동현,박승문 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.1
Rhodobacter sphaeroides has been well-known for its strong contribution to environment, medicine, food, and cosmetic industry. Here, we first reported the growthpromoting effects of an alginate oligosaccharide mixture (AOS) on R. sphaeroides. AOS exhibited a stimulatory effect on the growth of R. sphaeroides in a dose-dependent manner. The highest growth rate of R. sphaeroides was obtained in LB medium supplemented with 0.5% AOS. AOS exerted a selective growth-promoting effect on R. sphaeroides but not on Escherichia coli, Lysinibacillus xylanilyticus, and Pantoea rwandensis. The growth-promoting effect of AOS is influenced by light and may be involved in the up-regulation of fbp2, RSP-0557, and RegB genes. Our results collectively suggested that AOS should be employed as a growth-promoting factor to enhance the transition of R. sphaeroides to industrial applications.