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논에서 분리한 메탄산화세균 Methylomonas sp. SM4의 특성과 메탄올 생합성
박성민(Sung Min Park),Lavanya Madhavaraj,김시욱(Si Wouk Kim) 한국생물공학회 2017 KSBB Journal Vol.32 No.2
A methane-oxidizing bacterium was isolated from rice paddy field soil around Jeollanam-do province, Korea, and characterized. The isolate was gram-negative, orange pigmented and short rod (1.1-1.2 × 1.6-1.9 μm). It was catalase and urease-negative but oxidase-positive. The strain utilized methane and methanol as sole carbon and energy sources. It had an ability to grow with an optimum pH 7.0 and an optimum growth temperature 30℃. The strain was resistant to antibiotic polymyxin B but sensitive to streptomycin, kanamycin, ampicillin, chloramphenicol and rifampicin. The isolate required copper for their growth with concentration range of 2-25 μM, with an optimum of 10 μM. Under optimal culture condition, specific cell growth rate and generation time were found to be 0.046 hr<SUP>-1</SUP> and 15.13 hr, respectively. Phylogenetic analysis based on 16S rDNA sequences indicated that the strain formed a tight phylogenetic lineage with Methylomonas koyamae with a value of 99.4% gene sequence homology. So, we named the isolate as Methylomonas sp. SM4. 8.6 mM methanol was accumulated in the reaction mixture containing 70 mM sodium formate and 40 mM MgCl₂ (MDH inhibitor) under atmosphere of methane:air (40:60) mixture for 24 hr at 30℃.