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- 저자 : Laila Rashed (검색결과 3 건)
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Dexmedetomidine and propofol sedation requirements in an autistic rat model
Soha A. Elmorsy,Ghada F. Soliman,Laila A. Rashed,Hamed M. Elgendy 대한마취통증의학회 2019 Korean Journal of Anesthesiology Vol.72 No.2
Background: Autism is a challenging neurodevelopmental disorder. Previous clinical observations have suggested altered sedation requirements for children with autism. Our study aimed to test this observation experimentally in an animal model and to explore its possible mechanisms. Methods: Eight adult pregnant female Sprague-Dawley rats were randomly divided into two groups. Four were injected with intraperitoneal sodium valproate on gestational day 12 and four were injected with normal saline. On postnatal day 28, the newborn male rats were subjected to the open-field test to confirm autistic features. Each rat was injected intraperitoneally with a single dose of propofol (50 mg/kg) or dexmedetomidine (0.2 mg/kg). The times to loss of righting reflex (LORR) and to return of righting reflex (RORR) were recorded. On the following day, all rats were re-sedated and underwent electroencephalography (EEG). Thereafter, the rats were euthanized and their hippocampal gamma-aminobutyric acid type A (GABAA) and glutamate N-methyl-D-aspartate (NMDA) receptor gene expressions were assessed. Results: Autistic rats showed significantly longer LORR times and shorter RORR times than did the controls (median LORR times: 12.0 versus 5.0 min for dexmedetomidine and 22.0 versus 8.0 min for propofol; P < 0.05). EEG showed a low-frequency, high-amplitude wave pattern 2 min after LORR in the control rats. Autistic rats showed a high-frequency, low-amplitude awake pattern. Hippocampal GABAA receptor gene expression was significantly lower and NMDA gene expression was greater in autistic rats. Conclusions: This study supports the clinical observations of increased anesthetic sedative requirements in children with autism and our biochemical analyses using GABAA and glutamate receptor gene expression highlight possible underlying mechanisms.
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Higher Expression of Toll-like Receptors 3, 7, 8, and 9 in Pityriasis Rosea
Mostafa Abou El-Ela,Rania Abdel Hay,Mohamed El-Komy,Rehab Hegazy,Amin Sharobim,Laila Rashed,Khalda Amr 대한병리학회 2017 Journal of Pathology and Translational Medicine Vol.51 No.2
Background: Pityriasis rosea (PR) is a common papulosquamous skin disease in which an infective agent may be implicated. Toll-like receptors (TLRs) play an important role in immune responses and in the pathophysiology of inflammatory skin diseases. Our aim was to determine the possible roles of TLRs 3, 7, 8, and 9 in the pathogenesis of PR. Methods: Twenty-four PR patients and 24 healthy individuals (as controls) were included in this case control study. All recruits were subjected to routine laboratory investigations. Biopsies were obtained from one active PR lesion and from healthy skin of controls for the detection of TLR 3, 7, 8, and 9 gene expression using real-time polymerase chain reaction. Results: This study included 24 patients (8 females and 16 males) with active PR lesions, with a mean age of 28.62 years. Twenty four healthy age- and sex-matched individuals were included as controls (8 females and 16 males, with a mean age of 30.83 years). The results of the routine laboratory tests revealed no significant differences between both groups. Significantly elevated expression of all studied TLRs were detected in PR patients relative to healthy controls (p < .001). Conclusions: TLRs 3, 7, 8, and 9 might be involved in the pathogenesis of PR.
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Eman Mumtaz El Mahdy,Maha Gamal,Basma Emad Aboulhoda,Basent Adel Al Dreny,Ashraf Shamaa,Laila Rashed,Ahmed Nour Eldine Abdallah,Asmaa M. Shamseldeen 한국통합생물학회 2023 Animal cells and systems Vol.27 No.1
Parkinson’s disease (PD), the second common neurodegenerative disease, is characterized by theloss of the dopaminergic neurons in the substantia nigra (SN). Because of L-dopa’s side effects, newtherapeutic methods were considered such as stem cells for PD treatment. The major challengefacing stem cell therapy following transplantations is their peripheral sequestration anddecreased survival. Thus, the ability of erythropoietin (EPO) and low-level laser therapy (LLLT) toenhance mesenchymal stem cell (MSC) proliferation and therapeutic efficiency for alleviatingrotenone-induced PD was investigated. Therefore, we compared the influence of stem cellpreconditioning with erythropoietin versus LLLT on MSCs’ homing ability and the chances ofsurvival. Forty-eight male Swiss mice were included in the in vivo protocol and ten in the invitro work. The mice in the in vivo study were divided randomly into six groups (8 in each);group I; control group and group II; induced PD (untreated) group, group III; L-dopa, group IV;MSCs, group V; EPO-activated MSCs and group VI; laser-activated MSCs. Treatment with MSCs(either preconditioned or not) improved these neurological features by restoring the normalbalance between glutamate and GABA neurotransmitters, and dopamine as well as increasedtyrosine hydroxylase levels. Stem cell therapy decreased oxidative stress, miRNA-155 levels,enhanced neuronal architecture in SN, and decreased the number of apoptotic cells. LLLTenhanced MSC expression of integrin β1 which was reflected in the homing of PKH26-labelledMSCs into corpus striatum and SN. Thus, the optimum results were achieved with laseractivatedMSCs.
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