Three-month Treatment Response and Exacerbation in Chronic Obstructive Pulmonary Disease

Lee, Jung Su,Rhee, Chin Kook,Yoo, Kwang Ha,Lee, Ji-Hyun,Yoon, Ho Il,Kim, Tae-Hyung,Kim, Woo Jin,Lee, JinHwa,Lim, Seong Yong,Park, Tai Sun,Lee, Jae Seung,Lee, Sei Won,Lee, Sang-Do,Oh, Yeon-Mok The Korean Academy of Medical Sciences 2015 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.30 No.1

PKB phosphorylates p27, impairs its nuclear import and opposes p27 - mediated G1 arrest

JINHWA LEE,JIYONG LIANG,JOYCE M. SLINGERLAND 한국생명과학회 2002 한국생명과학회 심포지움 Vol.37 No.-

PKB activation may contribute to resistance to antiproliferative signals and breast cancer progression in part by impairing nuclear import and action of p27. PKB transfection caused cytoplasmic p27 accumulation and cytokine resistance. The nuclear localization region of p27 contains a PKB/Akt consensus site at threonine 157 and p27 phosphorylation by PKB impaired its nuclear import in vitro. PKB/Akt phosphorylated wild type p27 but not p27T157A. PKB activation led to cytoplasmic mislocalization of p27WT but p27T157A remained nuclear. In PKB activated cells, p27WT failed to cause G1 arrest, while the anti proliferative effect of p27T157 A was not impaired. Cytoplasmic p27 was seen in 41% (52/128) of primary human breast cancers in association with PKB activation. Thus, we show a novel mechanism whereby PKB impairs p27 function that is associated with an aggressive phenotype in human breast cancer.

Korean Children's Acquisition of English Interrogatives

Jinhwa Lee(이진화) 한국영어학회 2006 영어학 Vol.6 No.3

Structural resemblance of the DNAJA-family protein, Tid1, to the DNAJB-family Hsp40

Jinhwa Jang,Sung-Hee Lee,Dong-Hoon Kang,Dae-Won Sim,Kyung-Suk Ryu,Ku-Sung Jo,Jinhyuk Lee,Hyojung Ryu,Eun-Hee Kim,원형식,김지훈 생화학분자생물학회 2022 BMB Reports Vol.55 No.10

The specific pair of heat shock protein 70 (Hsp70) and Hsp40constitutes an essential molecular chaperone system involvedin numerous cellular processes, including the proper folding/refolding and transport of proteins. Hsp40 family members arecharacterized by the presence of a conserved J-domain (JD) thatfunctions as a co-chaperone of Hsp70. Tumorous imaginal disc1 (Tid1) is a tumor suppressor protein belonging to the DNAJA3subfamily of Hsp40 and functions as a co-chaperone of themitochondrial Hsp70, mortalin. In this work, we performed nuclearmagnetic resonance spectroscopy to determine the solutionstructure of JD and its interaction with the glycine/phenylalaninerichregion (GF-motif) of human Tid1. Notably, Tid1-JD, whoseconformation was consistent with that of the DNAJB1 JD, appearedto stably interact with its subsequent GF-motif region. Collectively with our sequence analysis, the present resultsdemonstrate that the functional and regulatory mode of Tid1resembles that of the DNAJB1 subfamily members rather thanDNAJA1 or DNAJA2 subfamily proteins. Therefore, it is suggestedthat an allosteric interaction between mortalin and Tid1is involved in the mitochondrial Hsp70/Hsp40 chaperone system.

Cyclophilin A as a New Therapeutic Target for Hepatitis C Virus-induced Hepatocellular Carcinoma

Lee, Jinhwa The Korean Society of Pharmacology 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.5

Hepatocellular carcinoma (HCC) related to hepatitis B virus (HBV) and hepatitis C virus (HCV) infections is thought to account for more than 80% of primary liver cancers. Both HBV and HCV can establish chronic liver inflammatory infections, altering hepatocyte and liver physiology with potential liver disease progression and HCC development. Cyclophilin A (CypA) has been identified as an essential host factor for the HCV replication by physically interacting with the HCV non structural protein NS5A that in turn interacts with RNA-dependent RNA polymerase NS5B. CypA, a cytosolic binding protein of the immunosuppressive drug cyclosporine A, is overexpressed in many cancer types and often associated with malignant transformation. Therefore, CypA can be a good target for molecular cancer therapy. Because of antiviral activity, the CypA inhibitors have been tested for the treatment of chronic hepatitis C. Nonimmunosuppressive Cyp inhibitors such as NIM811, SCY-635, and Alisporivir have attracted more interests for appropriating CypA for antiviral chemotherapeutic target on HCV infection. This review describes CypA inhibitors as a potential HCC treatment tool that is contrived by their obstructing chronic HCV infection and summarizes roles of CypA in cancer development.

Effects of Recasts in SLA: A Review for Research Synthesis

Jinhwa Lee 서울대학교 교육종합연구원 2005 The SNU Journal of Education Research Vol.14 No.-

L2 researchers have been investigating the role and effect of recasts as a type of negative feedback in SLA. The present study reviews sixteen empirical studies on recasts from a synthetic perspective. Since there are many moderator factors which may reduce or enhance the effect of recasts, this study examined and compared the studies in terms of 1) definition of recasts, 2) learner factors (age, proficiency, and developmental readiness), 3) language factors (morphemes or type of L2 structures), and 4) measures. Findings indicate that overall, recasts play a facilitative role in SLA and that the degree of effectiveness is dependent on the operationalization of recasts and the moderator variables. Suggestions are made for future research on recasts and their synthesis.

Anticancer Activity of <i>S</i>-Allylmercapto-<small>L</small>-cysteine on Implanted Tumor of Human Gastric Cancer Cell

Lee, Yongkyu,Kim, Hejin,Lee, Jinhwa,Kim, Kyongtai Pharmaceutical Society of Japan 2011 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.34 No.5

<P>Allylmercapto glutathione <I>S</I>-conjugate, <I>S</I>-allylmercapto-<SMALL>L</SMALL>-cysteine (SAMC), which is biotransformed from allyl sulfides and from naturally occurring water-soluble garlic derivatives, has been known to inhibit tumorigenesis. We found that SAMC was able to induce apoptosis in gastric cancer cells <I>in vitro</I>. We report that SAMC inhibited tumor growth rate by 31.36% and 37.78% at doses of 100 and 300 mg/kg, respectively. Apoptosis in the implanted tumor cells was manifested by apoptotic characteristics, including morphological changes of chromatin crescent, cell shrinkage and membrane blebbing. The apoptosis index of 100 mg/kg and 300 mg/kg of SAMC was 20.74±2.50% and 30.61±2.42%, respectively, by terminal deoxy-nucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL) staining. The positive rate of B-cell lymphoma 2 (bcl-2) protein expression of control, 100 mg/kg SAMC and 300 mg/kg SAMC was 15.20±1.67%, 10.94±1.57%, and 8.24±1.07%, respectively, by immunohistochemical staining. The positive rate of bax protein expression of control, 100 mg/kg SAMC and 300 mg/kg SAMC was 15.30±1.90%, 23.18±1.81%, and 25.26±3.03%, respectively. We also observed decreases in bcl-2 mRNA and increases in bax mRNA by SAMC in a dose-dependent manner by reverse transcription-polymerase chain reaction (RT-PCR). These results suggest that SAMC may regulate bcl-2 and bax to induce apoptosis in transplanted tumor cells.</P>

Establishment of normal canine thoracic radiograph database in small breed dogs

Jinhwa Chang,Wooshin Choi,Jinyoung Song,Youngjae Lee,Donghoon Lee,Juhyung Kim,장동우 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.3

Inter-breed and individual variations in thoracic conformation often resulted in incorrect diagnosis during interpreting canine thoracic radiographs. Therefore, it may be helpful to build up a collection of normal thoracic radiographs of different breeds for useful reference. The aim of this study is to establish the normal canine thoracic radiograph database according to breed, age and body weight. Medical records and thoracic radiographs of Veterinary Medical Center, Chungbuk National University were reviewed retrospectively. Normal thoracic radiographs of 170 dogs out of 640 patients that had thoracic radiographs were obtained. In 170 normal dogs, Maltese, Shih-tzu, Yorkshire Terriers, and Miniature Poodle were the most prevalent breeds with the number of 90 dogs. In this study, the normal canine thoracic radiograph database was established in the four breed, and it can be used for normal references when interpreting canine thoracic radiographs.

A Study on CeO2/SiO2 Composite Powder Synthesis Using Ultrasonic Spray Pyrolysis Method and Effect of Sensory Texture Improvement

Lee DongKyu,Lee JinHwa 한국유화학회 2005 한국응용과학기술학회지 Vol.22 No.2

The spherical particles of CeO_2/SiO_2 composite powder with narrow-size distribution and pure phase particles were synthesized by ultrasonic spray pyrolysis method from aqueous cerium sulfate solution. The resulting composite powder was characterized by X-ray diffraction, scanning electron microscopy, transmittance electron microscopy, in-vitro sun protect factor, and BET surface area analysis. The concentration of cerium sulfate was tested to vary the particle size from 3.40{times}10^{-3} to 1.02{times}10^{-2}mol/cm^3 to study concentration effect of starting material. The average particle size from the 3.40{times}10^{-3}mol/cm^3 concentration was found to be slightly smaller than that from the 1.02{times}10^{-2}mol/cm^3 concentration, because of the relation between the droplet size and the concentration of the starting material solution

Role of Cyclophilin A during Oncogenesis

Jinhwa Lee 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.2

Cyclophilins (Cyps) are ubiquitously expressed proteins that are evolutionarily conserved. CypA is the most abundant among the Cyps and is expressed in the cytosol. With its chaperone and PPIase activities, CypA contributes to the maintenance of correct conformation of nascent or denatured proteins and also provides protection against environmental insults. Also, its expression is induced in response to a wide variety of stressors including cancer. Upregulation of CypA in small cell lung cancer, pancreatic cancer, breast cancer, colorectal cancer, squamous cell carcinoma and melanoma has been reported. In some cancers a correlation between CypA overexpression and malignant transformation has been established. While molecular partners of CypA that promote cancer development are yet to be discovered, various mechanisms have been proposed to account for the cytoprotective functions of CypA during cancer development. CypA may promote the survival of cells under the stressful condition of cancer. CypA may well be essential for maintaining the conformation of oncogenic proteins,signalling proteins for cell proliferation, antiapoptotic components, transcription factors, or cell motility regulatory proteins. Antioxidant effects of CypA, which have been suggested by some researchers, may also become critical to reactive oxygen species (ROS) creating an oncogenetic environment. Developing new CypA inhibitors for therapeutics has been surmised from the cytoprotective functions of CypA and its overexpression in many cancer types. Therefore, CypA can be further investigated as a useful tool for early diagnosis, treatment and prevention of human cancers.