Small Angle Neutron Scattering Study of Nanostructural Changes in Microbial Transglutaminase-treated Low-fat Yogurt during Fermentation

Lívia Darnay,Adél Len,Ágota Koncz,László Friedrich,László Rosta 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6

Small angle neutron scattering (SANS) studies of fermentation of bovine milk with and without microbial transglutaminase (mTG) are described. Use of mTG increased the quality of low-fat yogurt via cross-linking of milk proteins. The SANS method was suitable for simultaneous characterization of the fermentation process and the effect of mTG. A decrease of whey protein release into the supernatant of centrifuged low-fat yogurt samples showed how mTG was capable to cross-link them already after 1 h of fermentation. The applied SANS method can be used to follow the sol-gel transformation of fermented dairy products in general. Moreover it can be a special tool for detecting the activity of mTG during fermentation of low-fat yogurts.

An in vitro model of Fusobacterium nucleatum and Porphyromonas gingivalis in single- and dual-species biofilms

Lívia Jacovassi Tavares,Marlise Inêz Klein,Beatriz Helena Dias Panariello,Erica Dorigatti de Avila,Ana Cláudia Pavarina 대한치주과학회 2018 Journal of Periodontal & Implant Science Vol.48 No.1

Purpose: The goal of this study was to develop and validate a standardized in vitro pathogenic biofilm attached onto saliva-coated surfaces. Methods: Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. gingivalis) strains were grown under anaerobic conditions as single species and in dual-species cultures. Initially, the bacterial biomass was evaluated at 24 and 48 hours to determine the optimal timing for the adhesion phase onto saliva-coated polystyrene surfaces. Thereafter, biofilm development was assessed over time by crystal violet staining and scanning electron microscopy. Results: The data showed no significant difference in the overall biomass after 48 hours for P. gingivalis in single- and dual-species conditions. After adhesion, P. gingivalis in single- and dual-species biofilms accumulated a substantially higher biomass after 7 days of incubation than after 3 days, but no significant difference was found between 5 and 7 days. Although the biomass of the F. nucleatum biofilm was higher at 3 days, no difference was found at 3, 5, or 7 days of incubation. Conclusions: Polystyrene substrates from well plates work as a standard surface and provide reproducible results for in vitro biofilm models. Our biofilm model could serve as a reference point for studies investigating biofilms on different surfaces.

A New Biotechnological Process to Enhance the Soymilk Bioactivity

Lívia Dias de Queirós,Juliana Alves Macedo,Gabriela Alves Macedo 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.3

Equol, a daidzein metabolite produced exclusively by intestinal bacteria in some, but not all, humans, exhibits a wide range of beneficial health effects owing to its superior nutraceutical effect compared with isoflavones of soy. The aim of this work was to develop bioprocesses capable of increasing the bioactive properties of soymilk and, most importantly, increase the equol content by a biotechnological process in vitro. Biotransformation processes based on soymilk fermentation by probiotic lactic bacteria and application of the enzyme tannase caused an increase in the bioactive isoflavones and antioxidant capacity of soymilk. Furthermore, these processes approximately resulted in a 10-fold increase in the equol content of the soymilk. This is the first study to produce a significant equol concentration in soymilk using enzymatic processing only. The results suggest a new and effective biotechnological process, with major commercial potential, capable of producing a bioactive soy extract that intends to be “functional for everyone.”

Treatment efficacy of gingival recession defects associated with non-carious cervical lesions: a systematic review

Lívia Maria Lopes de Oliveira,Camila Agra Souza,Sinara Cunha,Rafael Siqueira,Bruna de Carvalho Farias Vajgel,Renata Cimões 대한치주과학회 2022 Journal of Periodontal & Implant Science Vol.52 No.2

Purpose: This systematic review aimed to compare the efficacy, defined in terms of the mean percentage of root coverage (mRC), of surgical treatment approaches combined with adhesive restorations of non-carious cervical lesions (NCCLs) to that of root coverage alone in patients with a single gingival recession (GR) and NCCL. Methods: A literature search was conducted to identify longitudinal studies reporting the mRC following treatment for the correction of GR defects associated with NCCLs using a combination of surgical and restorative techniques in systemically and periodontally healthy patients. Results: The search resulted in the retrieval of 12,409 records. Seven publications met the inclusion criteria for the qualitative synthesis of data. The mRCs ranged from 69% to 97%. In the medium term, the gingival margin position was more stable when a connective tissue graft (CTG) was used, independently of whether restoration of teeth with NCCLs was performed. Conclusions: The strength of the evidence was limited by methodological heterogeneity in terms of study design as well as the unit and period of analysis, which precluded a metaanalysis. Although no definitive conclusion could be drawn due to the lack of sufficient evidence to estimate the effectiveness of the interventions, CTG-based procedures contributed to gingival margin stability regardless of the performance of restoration to treat NCCLs.

Oral methylmercury intoxication aggravates cardiovascular risk factors and accelerates atherosclerosis lesion development in ApoE knockout and C57BL/6 mice

Silva Janayne L.,Leocádio Paola C. L.,Reis Jonas M.,Campos Gianne P.,Capettini Luciano S. A.,Foureaux Giselle,Ferreira Anderson J.,Windmöller Cláudia C.,Santos Flávia A.,Oriá Reinaldo B.,Crespo-López 한국독성학회 2021 Toxicological Research Vol.37 No.3

Methylmercury (MeHg) intoxication is associated with hypertension, hypercholesterolemia, and atherosclerosis by mechanisms that are not yet fully understood. We investigated the effects of MeHg intoxication in atherosclerosis-prone (ApoE-KO) and resistant C57BL/6 mice. Mice were submitted to carotid stenosis surgery (to induce atherosclerosis faster) and received water or MeHg solution (20 mg/L) for 15 days. Tail plethysmography was performed before and after MeHg exposure. Food and MeHg solution intakes were monitored weekly. On the 15th day, mice were submitted to intravital fluorescence microscopy of mesenteric vasculature to observe in vivo leukocyte rolling and adhesion. Results showed that despite the high hair and liver Hg concentrations in the MeHg group, food and water (or MeHg solution) consumption and liver function marker levels were similar to those in controls. MeHg exposure increased total cholesterol, the atherogenic (non-HDL) fraction and systolic and diastolic blood pressure. MeHg exposure also induced inflammation, as seen by the increased rolling and adhered leukocytes in the mesenteric vasculature. Atherosclerosis lesions were more extensive in the aorta and carotid sites of MeHg-ApoE knockout mice. Surprisingly, MeHg exposure also induced atherosclerosis lesions in C57BL/6 mice, which are resistant to atherosclerosis formation. We concluded that MeHg intoxication might represent a risk for cardiovascular diseases since it accelerates atherogenesis by exacerbating several independent risk factors.

가상이라는 조건

리비아 놀라스코-로자스(Lívia Nolasco-Rózás) 이응노미술관 2020 고암논총 Vol.9 No.-

Antioxidant Activity and Phenolic and Mineral Content of Rose Grape Juice

Caroline Dani,Lívia S. Oliboni,Regina Vanderlinde,Daniel Pra,Johnny F. Dias,Maria Lucia Yoneama,Diego Bonatto,Mirian Salvador,João A.P. Henriques 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.1

There are many studies related to the antioxidant activity of grape products; however, they concern only purple and white grape varieties. Up to now, there are no reports of studies on the Goethe rose grape variety, either on its antioxidant activity or on its phenolic and mineral quantification. Thus, the purpose of this study was to evaluate in vitro and in vivo antioxidant activity, as well as to quantify total phenolic compounds, ascorbic acid, and mineral content, in a Goethe rose grape juice. The results obtained showed that the Goethe rose grape juice is a great polyphenol source, which contains catechin, epicatechin, and procyanidins (B1, B2, B3, and B4). Of all metals analyzed, potassium, calcium, magnesium, and iron showed the highest values. We found that this rose grape juice shows an important antioxidant activity in in vitro (2,2-diphenyl-1-picrylhydrazyl radical scavenging activity) and in vivo (using the Saccharomyces cerevisiae yeast cells) assays. The antioxidant activity could be explained by the significant phenolic content and ascorbic acid levels found in the juice. The results showed that rose grape juice is an excellent antioxidant source, which could contribute to the prevention of many diseases related to oxidative stress, such as atherosclerosis and Parkinson's disease.

Antioxidant and Antigenotoxic Activities of Purple Grape Juice—Organic and Conventional—in Adult Rats

Caroline Dani,Lívia S. Oliboni,Fernanda M. Umezu,Matheus A.B. Pasquali,Mirian Salvador,José Cláudio Fonseca Moreira,João Antonio Pegas Henriques 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.5

Oxidative damage to biomolecules occurs by the accumulation of molecular damage due to free radicals and/or a diminution of antioxidant protection. The aim of this study was to evaluate the protection of organic and conventional purple grape juices in brain, liver, and plasma from adult Wistar rats (7 months old) against the oxidative damage provoked by carbon tetrachloride (CCl4). Adult rats were divided into three groups (control, conventional purple grape juice, and organic purple grape juice). Half of the rats received CCl4, and the other half received the vehicle (vegetable oil). The chemical analytical determination showed that the highest levels of total phenolic, resveratrol, and catechins were seen in organic purple grape juices. Considering the treatment groups, it was observed that in all tissues (brain structures and liver) and plasma, CCl4 treatment increased the lipid peroxidation (LP) levels. Both grape juices were capable to reduce LP levels in cerebral cortex and hippocampus; however, in the striatum and substantia nigra only the organic grape juice reduced LP level. CCl4 caused an increase in catalase activity in cerebral cortex, hippocampus, and substantia nigra and in superoxide dismutase activity in substantia nigra. This increase was reduced by both juices in substantia nigra and hippocampus structures (P<.05). In the alkaline version of the comet assay performed on whole blood, it was observed that CCl4 was capable of inducing mainly DNA damage class 4 and 3 frequencies, which was significantly reduced in groups that received both purple grape juices. This implies that both grape juices have an important antigenotoxic activity.

Rice Iron Metabolism: from Source to Solution

Antonio Costa de Oliveira,Lívia Scheunemann dos Santos 한국작물학회 2007 Journal of crop science and biotechnology Vol.10 No.2

Iron is an important micronutrient for plants. Iron metabolism is a complex mechanism under a delicate balance. Iron metabolism represents two major problems for plants: deficiency as a consequence of solubility problems and toxicity due to excess solubility in anaerobic conditions. In the last few years, new genes have been discovered that influence iron uptake, transport, and storage. Irrigated rice is exposed to high levels of FeII, normally rare in aerobic soil conditions. The implications of altering iron uptake rates and the effects of newly discovered genes are discussed Iron is an important micronutrient for plants. Iron metabolism is a complex mechanism under a delicate balance. Iron metabolism represents two major problems for plants: deficiency as a consequence of solubility problems and toxicity due to excess solubility in anaerobic conditions. In the last few years, new genes have been discovered that influence iron uptake, transport, and storage. Irrigated rice is exposed to high levels of FeII, normally rare in aerobic soil conditions. The implications of altering iron uptake rates and the effects of newly discovered genes are discussed

Effect of intracanal cryotherapy on postoperative pain after endodontic treatment: systematic review with meta-analysis

Hespanhol Fernanda Garcias,Guimarães Ludmila Silva,Antunes Lívia Azeredo Alves,Antunes Leonardo Santos 대한치과보존학회 2022 Restorative Dentistry & Endodontics Vol.47 No.3

Objectives This study aimed to evaluate the effectiveness of final irrigation with cold saline solution after endodontic treatment compared with saline solution at room temperature against postoperative pain following endodontic treatment. Materials and Methods A broad search was performed in the PubMed, Web of Science, Scopus, Cochrane Library, Virtual Health Library (LILACS), and Grey Literature databases. Two independent reviewers performed data extraction, risk of bias using the Cochrane methodology, and certainty of evidence using the Grading of Recommendations, Assessment, Development and Evaluations (GRADE) approach. Results Eight studies were included in qualitative synthesis. Intracanal cryotherapy favored the reduction of postoperative pain in the systematic review. Four studies were included in meta-analyses. The meta-analysis showed that intracanal cryotherapy reduced postoperative pain in teeth with symptomatic apical periodontitis (SAP) at 24 hours. There was no association between intracanal cryotherapy and control (room temperature) groups in teeth with normal periapical tissue with respect to postoperative pain at 24 hours and 48 hours. Conclusions Intracanal cryotherapy was effective in reducing postoperative pain after endodontic treatment in teeth with SAP. Objectives This study aimed to evaluate the effectiveness of final irrigation with cold saline solution after endodontic treatment compared with saline solution at room temperature against postoperative pain following endodontic treatment. Materials and Methods A broad search was performed in the PubMed, Web of Science, Scopus, Cochrane Library, Virtual Health Library (LILACS), and Grey Literature databases. Two independent reviewers performed data extraction, risk of bias using the Cochrane methodology, and certainty of evidence using the Grading of Recommendations, Assessment, Development and Evaluations (GRADE) approach. Results Eight studies were included in qualitative synthesis. Intracanal cryotherapy favored the reduction of postoperative pain in the systematic review. Four studies were included in meta-analyses. The meta-analysis showed that intracanal cryotherapy reduced postoperative pain in teeth with symptomatic apical periodontitis (SAP) at 24 hours. There was no association between intracanal cryotherapy and control (room temperature) groups in teeth with normal periapical tissue with respect to postoperative pain at 24 hours and 48 hours. Conclusions Intracanal cryotherapy was effective in reducing postoperative pain after endodontic treatment in teeth with SAP.