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PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis
Lee, Kwanwoo,Rhee, Kunsoo The Rockefeller University Press 2011 The Journal of cell biology Vol.195 No.7
<P>The microtubule-organizing activity of the centrosome oscillates during the cell cycle, reaching its highest level at mitosis. At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar matrix (PCM) and a robust increase in microtubule-organizing activity. It is known that PLK1 is critical for the initiation of centrosome maturation. In this paper, we report that pericentrin (PCNT), a PCM protein, was specifically phosphorylated by PLK1 during mitosis. Phosphoresistant point mutants of PCNT did not recruit centrosomal proteins, such as CEP192, GCP-WD (γ-complex protein with WD repeats), γ-tubulin, Aurora A, and PLK1, into the centrosome during mitosis. However, centrosomal recruitment of CEP215 depended on PCNT irrespective of its phosphorylation status. Furthermore, ectopic expression of PLK1-PCNT fusion proteins induced the centrosomal accumulation of CEP192, GCP-WD, and γ-tubulin even in interphase cells, mimicking centrosome maturation. Based on these results, we propose that PLK1-mediated phosphorylation of PCNT initiates centrosome maturation by organizing the spindle pole–specific PCM lattice.</P>
Lee, Jang-Yong,Jung, Jin-Mook,Kim, Do-Young,Lee, Young Seak,Jung, Chan-Hee,Shin, Kwanwoo,Choi, Jae-Hak American Scientific Publishers 2017 Journal of Nanoscience and Nanotechnology Vol.17 No.8
<P>In this study, conductive carbon thin films were prepared from poly(vinyl alcohol) (PVA) thin films through iodine pretreatment and pyrolysis. PVA thin films spin-coated on a substrate were iodinated with an aqueous NH4I/I-2 solution. The iodinated PVA thin films were stabilized in air and then pyrolyzed under inert atmosphere to form carbon thin films. As a result, iodine pretreatment was found to enhance the thermal stability of the PVA. Carbon films with imperfect graphitic structures were formed by pyrolysis of the stabilized PVA films containing polyene structures. The carbon films showed a high electrical conductivity of 2.00x10(2) S/cm and a greatly low surface roughness of below 1.0 nm.</P>
Lee, Keel Yong,Park, Sung-Jin,Lee, Keon Ah,Kim, Se-Hwan,Kim, Heeyeon,Meroz, Yasmine,Mahadevan, L,Jung, Kwang-Hwan,Ahn, Tae Kyu,Parker, Kevin Kit,Shin, Kwanwoo Nature Publishing Group, a division of Macmillan P 2018 Nature biotechnology Vol.36 No.6
<P>Inside cells, complex metabolic reactions are distributed across the modular compartments of organelles(1,2). Reactions in organelles have been recapitulated in vitro by reconstituting functional protein machineries into membrane systems(3-5). However, maintaining and controlling these reactions is challenging. Here we designed, built, and tested a switchable, light-harvesting organelle that provides both a sustainable energy source and a means of directing intravesicular reactions. An ATP (ATP) synthase and two photoconverters (plant-derived photosystem II and bacteriaderived proteorhodopsin) enable ATP synthesis. Independent optical activation of the two photoconverters allows dynamic control of ATP synthesis: red light facilitates and green light impedes ATP synthesis. We encapsulated the photosynthetic organelles in a giant vesicle to form a protocellular system and demonstrated optical control of two ATP-dependent reactions, carbon fixation and actin polymerization, with the latter altering outer vesicle morphology. Switchable photosynthetic organelles may enable the development of biomimetic vesicle systems with regulatory networks that exhibit homeostasis and complex cellular behaviors.</P>
Stress-Strain Behavior of the Electrospun Thermoplastic Polyurethane Elastomer Fiber Mats
Lee Keunhyung,Lee Bongseok,Kim Chihun,Kim Hakyong,Kim Kwanwoo,Nah Changwoon The Polymer Society of Korea 2005 Macromolecular Research Vol.13 No.5
Thermoplastic polyurethane elastomer (TPUe) fiber mats were successfully fabricated by electrospinning method. The TPUe fiber mats were subjected to a series of cycling tensile tests to determine the mechanical behavior. The electrospun TPUe fiber mats showed non-linear elastic and inelastic characteristics which may be due to slippage of crossed fiber (non-bonded or physical bonded structure) and breakage of the electro spun fibers at junctions (point-bonded or chemical bonding structure). The scanning electron microscopy (SEM) images demonstrated that the point-bonded structures of fiber mats played an important role in the load-bearing component as determined in loading-unloading component tests, which can be considered to have a force of restitution.
Lee, Jihye,Yoon, Donghwan,Shin, Kwanwoo,Kim, Kang-Jin,Lee, Yeonhee John Wiley Sons, Ltd. 2010 Surface and interface analysis Vol.42 No.8
<P>A surface-induced orientation of symmetric, deuterated polystyrene/poly(propyl methacrylate) diblock copolymers, dPS-PPrMA with different molecular weights (M<SUB>n</SUB> = 135 000, 119 600, and 75 300) was investigated using time-of-flight secondary ion mass spectrometry (TOF-SIMS). Elemental depth profiles obtained in the negative ion mode by a Cs<SUP>+</SUP> primary ion beam demonstrate variations in hydrogen, deuterium, carbon, oxygen, and hydrocarbons within the diblock copolymer according to the depth. Solution casting films of the copolymers with thicknesses of 700 and 2100 Å exhibited no preferential orientation of the microdomain morphology with respect to the surface at room temperature. Annealing the copolymer films at 215 °C for 4 h produced a dramatic orientation of the microdomains parallel to the surface of the film. This preferential alignment provided regular oscillation in the composition of both blocks, which continued through the entire film. The periodicity as determined from a depth profile of TOF-SIMS showed good agreement with the results of small-angle X-ray scattering. Positive and negative ion molecular depth profiles by a C<SUB>60</SUB><SUP>+</SUP> cluster ion beam also provide information pertaining to the lower disorder–order transition (LDOT) behavior of the dPS-PPrMA copolymer. Depth profile results indicate a PPrMA layer preferentially located at the copolymer/silicon substrate interface. The microdomain separation processes of dPS-PPrMA were investigated as a function of the annealing temperature and time. Copyright © 2010 John Wiley & Sons, Ltd.</P>
Development and Validation Study of the Internet Overuse Screening Questionnaire
HanKyeong Lee,HaeWoo Lee,JooHyun Han,Subin Park,SeokJin Ju,Kwanwoo Choi,JiHyeon Lee,HongJin Jeon 대한신경정신의학회 2018 PSYCHIATRY INVESTIGATION Vol.15 No.4
Objective-Concerns over behavioral and emotional problems caused by excessive internet usage have been developed. This study intended to develop and a standardize questionnaire that can efficiently identify at-risk internet users through their internet usage habits. Methods-Participants (n=158) were recruited at six I-will-centers located in Seoul, South Korea. From the initial 36 questionnaire item pool, 28 preliminary items were selected through expert evaluation and panel discussions. The construct validity, internal consistency, and concurrent validity were examined. We also conducted Receiver Operating Curve (ROC) analysis to assess diagnostic ability of the Internet Overuse Screening-Questionnaire (IOS-Q). Results-The exploratory factor analysis yielded a five factor structure. Four factors with 17 items remained after items that had unclear factor loading were removed. The Cronbach’s alpha for the IOS-Q total score was 0.91, and test-retest reliability was 0.72. The correlation between Young’s internet addiction scale and K-scale supported concurrent validity. ROC analysis showed that the IOS-Q has superior diagnostic ability with the Area Under the Curve of 0.87. At the cut-off point of 25.5, the sensitivity was 0.93 and specificity was 0.86. Conclusion-Overall, this study supports the use of IOS-Q for internet addiction research and for screening high-risk individuals.
광학 필름을 이용한 입체감 부여 자동차 실내 무드 조명 개발
이관우(Kwanwoo Lee),최재섭(Jaeseob Choi),강병영(Byeongyeong Kang) 한국자동차공학회 2019 한국자동차공학회 학술대회 및 전시회 Vol.2019 No.11
본 논문에서는 다양화 되고 있는 자동차 실내 무드 조명 디자인 트렌드에 맞춰 디자인 다양성을 확보하기 위한 신규 조명모듈 구조에 대해 논하고자 한다. 신규 작동 구조 검토에 앞서 현제까지 양산 또는 선행개발을 통해 보편적으로 적용되고 있는 실내 무드 조명 구조를 분석하고, 입체감 부여를 위한 광학필름의 인자별 영향도 검증을 실시하였다. 검토된 조건을 바탕으로 이를 개선/회피 하기 위한 신규 구조 도출, 3D 입체감 최적화를 위한 구조 조건 내에서 조명 성능 확보가 가능한 신규 조명 가니쉬 모델을 설계하였다. 조명 검증은 조명 해석 및 평가를 통해 진행하였다. 광학필름 및 조명모듈 설계 데이터를 바탕으로 각 영향 인자별 3D효과 영향도 사전검증 및 최종 데이터기준 조명 성능 해석까지 진행하였고, 시작금형 및 NC가공을 통해 제품 제작을 실시하여 실 제품단위에서 조명성능(3D효과구현, 휘도, 균일도)을 검증하였다. 이와 같은 검증 과정을 통해 광학 필름을 이용한 입체감 부여 자동차 실내 무드 조명 가니쉬 구조를 제안하고자 한다.