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RISS 인기검색어
- 검색키워드
- 저자 : Kumar Katya (검색결과 8 건)
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Katya, Kumar,Yun, Yong-Hyun,Park, Gunhyun,Lee, Jeong-Yeol,Yoo, Gwangyeol,Bai, Sungchul C. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.10
The present experiment was conducted to evaluate the efficacy of dietary fermented by-product of mushroom, Pleurotus ostreatus, (FBPM) as a fish meal (FM) replacer in juvenile Amur catfish, Silurus asotus. A total number of 225 fish averaging $5.7{\pm}0.1g$ ($mean{\pm}standard$ deviation) were fed one of the five experimental diets formulated to replace FM with FBPM at 0%, 5%, 10%, 20%, and 30% ($FBPM_0$, $FBPM_5$, $FBPM_{10}$, $FBPM_{20}$, and $FBPM_{30}$, respectively). At the end of eight weeks of the experiment, average weight gain (WG) of fish fed $FBPM_0$ or $FBPM_5$ were significantly higher than those of fish fed $FBPM_{20}$ or $FBPM_{30}$ diets (p<0.05). However, there was no significant differences in WG among the fish fed $FBPM_0$, $FBPM_5$ or $FBPM_{10}$, and between fish fed $FBPM_{10}$ or $FBPM_{20}$, and also between those fed $FBPM_{20}$ or $FBPM_{30}$ diets. Lysozyme activity of fish fed $FBPM_0$ or $FBPM_5$ were significantly higher than those of fish fed $FBPM_{10}$, $FBPM_{20}$ or $FBPM_{30}$ diets (p<0.05). The chemiluminescent response of fish fed $FBPM_5$ was significantly higher than those of fish fed $FBPM_0$, $FBPM_{20}$ or $FBPM_{30}$ diets (p<0.05). Broken line regression analysis of WG suggested that the maximal dietary inclusion level for FBPM as a FM replacer could be 6.3% without any adverse effects on whole body composition and on serological characteristics. Therefore, these results may indicate that the maximal dietary inclusion level of FBPM as a FM replacer could be 6.3% in juvenile Amur catfish.
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Lee, Seunghan,Katya, Kumar,Hamidoghli, Ali,Hong, Jeongwhui,Kim, Dae-Jung,Bai, Sungchul C. Elsevier 2018 FISH AND SHELLFISH IMMUNOLOGY Vol.83 No.-
<P><B>Abstract</B></P> <P>This study evaluated the synergistic effects of dietary <I>Bacillus subtilis</I> WB60 and mannanoligosaccharide (MOS) in juvenile Japanese eel, <I>Anguilla japonica</I>. Seven treatment diets were formulated to contain three different levels of <I>B. subtilis</I> (0.0, 0.5, and 1.0 × 10<SUP>7</SUP> CFU/g diet denoted as BS<SUB>0</SUB>, BS<SUB>0.5</SUB>, and BS<SUB>1</SUB>, respectively) with two MOS levels (0 and 5 g/kg diet denoted as M<SUB>0</SUB> and M<SUB>5</SUB>, respectively), and one diet with oxytetracycline (OTC) at 5 g/kg diet. Each diet (BS<SUB>0</SUB>M<SUB>0</SUB> (CON), BS<SUB>0</SUB>M<SUB>5</SUB>, BS<SUB>0.5</SUB>M<SUB>0,</SUB> BS<SUB>0.5</SUB>M<SUB>5,</SUB> BS<SUB>1</SUB>M<SUB>0,</SUB> BS<SUB>1</SUB>M<SUB>5</SUB>, and OTC) was fed to triplicate groups of 20 fish averaging 9.00 ± 0.11 g (mean ± SD) for eight weeks. Average weight gain, feed efficiency, specific growth rate and protein efficiency ratio of fish fed the BS<SUB>0.5</SUB>M<SUB>5</SUB> and BS<SUB>1</SUB>M<SUB>5</SUB> diets were significantly higher than those of fish fed CON, BS<SUB>0.5</SUB>M<SUB>0</SUB> and OTC diets (<I>P</I> < 0.05). Significant increases in the nonspecific enzymatic activities (e.g., lysozyme and myeloperoxidase) were detected from fish fed the BS<SUB>0.5</SUB>M<SUB>5</SUB>, BS<SUB>1</SUB>M<SUB>5</SUB>, and OTC diets compared to the CON, BS<SUB>0.5</SUB>M<SUB>0</SUB>, and BS<SUB>0</SUB>M<SUB>5</SUB> diets (<I>P</I> < 0.05). Whereas, immunoglobulin M expressions were recorded significantly higher for fish fed the BS<SUB>0.5</SUB>M<SUB>5</SUB> and BS<SUB>1</SUB>M<SUB>5</SUB> diets compared to those of fish fed the other diets (<I>P</I> < 0.05). Also, heat shock protein 70 mRNA levels of fish fed BS<SUB>0.5</SUB>M<SUB>5</SUB> and BS<SUB>1</SUB>M<SUB>5</SUB> diets were significantly higher than those of fish fed the CON diet (<I>P</I> < 0.05). Histological observations of the intestinal morphology showed healthier gut for fish fed BS<SUB>0.5</SUB>M<SUB>5</SUB> and BS<SUB>1</SUB>M<SUB>5</SUB> diets than those fed CON, BS<SUB>0</SUB>M<SUB>5</SUB>, and OTC diets. Additionally, resistance to bacterial challenge with <I>Vibrio anguillarum</I> was recorded significantly lower for fish fed the CON diet than those fed other diets (<I>P</I> > 0.05). Therefore, the results for growth performance, non-specific immune responses, intestinal morphology, and disease resistance demonstrated that supplementation of <I>B. subtilis</I> at 0.5 × 10<SUP>7</SUP> CFU/g diet and mannanoligosaccharide at 5 g/kg diet could have beneficial synergistic effects in Japanese eel. The isolated probiotic from eel and the selected prebiotic could lead to the development of a specific and potential synbiotic in Japanese eel aquaculture.</P> <P><B>Highlights</B></P> <P> <UL> <LI> •Higher growth and immunity could be achieved by the administration of <I>Busilus subitlis</I> and mannanoligosaccharide. </LI> <LI> •Dietary synbiotic could have more benefits than probiotic and prebiotic alone. </LI> <LI> •Specific synbiotic has the potential to replace chemical antibiotics in Japanese eel aquaculture. </LI> </UL> </P>
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Lee, Seunghan,Katya, Kumar,Park, Youngjin,Won, Seonghun,Seong, Minji,hamidoghli, Ali,Bai, Sungchul C. Elsevier 2017 FISH AND SHELLFISH IMMUNOLOGY Vol.61 No.-
<P><B>Abstract</B></P> <P>The current experiment was conducted to evaluate and compare the efficacy of two different probiotics <I>Bacillus subtilis</I> WB60 and <I>Lactobacillus plantarum</I> KCTC3928 in diet of Japanese eel, <I>Anguilla japonica</I>. Seven experimental diets were formulated to contain no probiotics (CON), three graded levels of <I>B. subtilis</I> at 10<SUP>6</SUP> (BS<SUB>1</SUB>), 10<SUP>7</SUP> (BS<SUB>2</SUB>), 10<SUP>8</SUP> (BS<SUB>3</SUB>) and <I>L. plantarum</I> at 10<SUP>6</SUP> (LP<SUB>1</SUB>), 10<SUP>7</SUP> (LP<SUB>2</SUB>), 10<SUP>8</SUP> (LP<SUB>3</SUB>) CFU/g diet. Twenty fish averaging 8.29 ± 0.06 g were distributed in to 21 aquaria and were randomly assigned to one of the experimental diets in triplicate groups. Average weight gain (WG), feed efficiency (FE), and protein efficiency ratio (PER) of fish fed <I>B. subtilis</I> at 10<SUP>7</SUP> (BS<SUB>2</SUB>) and 10<SUP>8</SUP> (BS<SUB>3</SUB>) CFU/g diet were significantly higher than those of fish fed other experimental diets (<I>P</I> < 0.05). Nonspecific enzymatic activities including lysozyme, superoxide dismutase (SOD), myeloperoxidase (MPO) from fish fed <I>B. subtilis</I> at 10<SUP>7</SUP> (BS<SUB>2</SUB>) and 10<SUP>8</SUP> (BS<SUB>3</SUB>) CFU/g diet were detected to be significantly higher than that from fish fed CON diet (<I>P</I> < 0.05). Whereas, level of intestine glyceraldehyde-3-phosphate dehydrogenase (GAPDH), heat shock protein 70, 90 (HSP70, 90) and immunoglobulin (IgM) from fish fed <I>B. subtilis</I> at 10<SUP>7</SUP> and 10<SUP>8</SUP> CFU/g diet were recorded to be significantly higher than those of fish fed other experimental diets (<I>P</I> < 0.05). Parameters for intestinal morphology and histology suggested a healthier gut for the fish fed10<SUP>8</SUP> (BS<SUB>3</SUB>) CFU/g diet as compared to other treatment groups. Whereas, results from the disease challenge test with bacteria <I>Vibrio angulillarum</I> showed significantly lower survival rate for fish fed CON diet than those of fish fed other experimental diets. Therefore, these results indicated that oral supplement of <I>B. subtilis</I> at 10<SUP>8</SUP> (BS<SUB>3</SUB>) CFU/g diet could be a more effective source of probiotic compared to <I>L. plantarum</I> in Japanese eel.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Preliminary report comparing two probiotics, <I>Bacillus subtilis</I> WB60 and <I>Lactobacillus plantarum</I> KCTC3928. </LI> <LI> Differences on the growth performance, survival rate, immune parameters and gut morphology were clearly observed. </LI> <LI> Oral administration of bacteria spp. <I>B. subtilis</I> at 10<SUP>7</SUP> CFU/g kg<SUP>−</SUP> <SUP>1</SUP> diet could be a more promising source and dose compared to probiotic <I>L. plantarum</I> in freshwater aquaculture. </LI> </UL> </P>
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윤현호,박건준,Imho Ok,Kumar Katya,Silas Heung,배승철 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.4
This study was carried out to evaluate the dietary threonine requirement by measuring the plasma free threonine and ammonia concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A total of 70 fish (average initial weight 506±8.2 g) were randomly distributed into each of the 14 net cages (5 fish/cage). After 48 hours (h) of feed deprivation, each group was intubated at 1% body weight with one of the seven L-amino acid based diets containing graded levels of threonine (0.42%, 0.72%, 0.92%, 1.12%, 1.32%, 1.52%, or 1.82% of diet, dry matter basis). Blood samples were taken at 0, 5, and 24 h after intubation. Post-prandial plasma free threonine concentrations (PPthr) of fish 5 h after intubation with diets containing 1.32% or more threonine were significantly higher than those of fish intubated with diets containing 1.12% or less threonine (p<0.05). Post-absorptive free threonine concentrations (PAthr) after 24 h of intubation of the fish with diets containing 0.92% or more threonine were significantly higher than those of fish intubated with diets containing 0.72% or less threonine. Post-prandial plasma ammonia concentrations (PPA, 5 h after intubation) were not significantly different among fish intubated with diets containing 1.12% or less threonine, except the PPA of fish intubated with diet containing 0.42% threonine. Broken-line model analyses of PPthr, PAthr, and PPA indicated that the dietary threonine requirement of rainbow trout should be between 0.95% (2.71) and 1.07% (3.06) of diet (% of dietary protein on a dry matter basis).
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Yun, Hyeonho,Park, Gunjun,Ok, Imho,Katya, Kumar,Heung, Silas,Bai, Sungchul C. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.4
This study was carried out to evaluate the dietary threonine requirement by measuring the plasma free threonine and ammonia concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A total of 70 fish (average initial weight $506{\pm}8.2g$) were randomly distributed into each of the 14 net cages (5 fish/cage). After 48 hours (h) of feed deprivation, each group was intubated at 1% body weight with one of the seven L-amino acid based diets containing graded levels of threonine (0.42%, 0.72%, 0.92%, 1.12%, 1.32%, 1.52%, or 1.82% of diet, dry matter basis). Blood samples were taken at 0, 5, and 24 h after intubation. Post-prandial plasma free threonine concentrations (PPthr) of fish 5 h after intubation with diets containing 1.32% or more threonine were significantly higher than those of fish intubated with diets containing 1.12% or less threonine (p<0.05). Post-absorptive free threonine concentrations (PAthr) after 24 h of intubation of the fish with diets containing 0.92% or more threonine were significantly higher than those of fish intubated with diets containing 0.72% or less threonine. Post-prandial plasma ammonia concentrations (PPA, 5 h after intubation) were not significantly different among fish intubated with diets containing 1.12% or less threonine, except the PPA of fish intubated with diet containing 0.42% threonine. Broken-line model analyses of PPthr, PAthr, and PPA indicated that the dietary threonine requirement of rainbow trout should be between 0.95% (2.71) and 1.07% (3.06) of diet (% of dietary protein on a dry matter basis).
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