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Huan Zhang,Kuijun Zhao,Dong Fan 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
β-N-acetylglucosaminidase plays an important role in insect chitin degradation. In this study, a novel cDNA sequence encoding β-N-acetylglucosaminidase (MbNAG) was cloned from cabbage moth, Mamestra brassicae L. The cDNA sequence, which was 2445 bp in length, contained an open reading frame of 1782 bp coding for a polypeptide of 594 amino acid residues. The MbNAG open reading frame was cloned into the pET-21b prokaryotic expression vector, and then over-expressed in Escherichia coli BL21 (DE3) cells. After purification and renaturation, the β-N-acetylglucosaminidase enzymatic activity was detected. Transcript analyses on MbNAG sequence during different developmental stages and in specific tissues were carried out by RT-qPCR. The MbNAG mRNA level was significantly higher of the second day in the prepupal stage than at any other time and occurred mainly in the midguts and salivary glands compared with in other tissues, including fat bodies, integuments, malpighian tubules, foreguts and hindguts during the feeding stage of the 5th instar larvae. The highest expression level of MbNAG mRNA occurred 48 h after an in vivo injection of 20-hydroxyecdysone. RNA interference reduced the MbNAG mRNA expression level 60% at 48 h and 67% at 72 h, respectively, and induced both abnormal molting phenotypes and a high mortality rate in M. brassicae larvae. These results suggest that MbNAG plays an essential
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A morphological study on autumnal morphs of Aphis glycines (Hemiptera: Aphididae)
Zhen-qi Tian,Su-ji Wang,Bing Bai,Jian Liu,Kuijun Zhao 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2
The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is one of the most important pests of soybean. The complex life cycle of A. glycines is characterized as heteroecious and holocyclic, and has seasonal polymorphisms occurring during its life cycle. In the autumn, A. glycines occur as winged and wingless virginoparae in soybeans, as gynoparae and males that migrate from soybeans to Rhamnus spp. and as gynoparae, males and oviparae on Rhamnus spp. In this study, wingless virginoparae, gynoparae, males and oviparae of A. glycines were successfully induced in the laboratory and morphological parameters of these morphs were selected for quantitation. To aid in identification of these A. glycines autumnal morphs, these aphids were imaged by microscope and significant differences in morphological characteristics were found: distal parts of hind leg femurs of gynoparae were grayish black, which were darker than those of winged virginoparae; the 4th instars of gynoparae, males and winged virginoparae differ greatly in dorsal abdomen coloration and covering. Our results provide an important guide for distinguishing adults of gynoparae and winged virginoparae, and for identifying nymphs of gynoparae, males and winged virginoparae.
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