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UV , TPA , heterologous DNA inserts 가 동물세포에서 homologous recombination 에 미치는 영향
송규영,Kucherlapati, Raju 한국유전학회 1992 Genes & Genomics Vol.14 No.1
We have studied the plasmid x plasmid recombination system to examine factors that might increase the frequency of homologous recombination. Treatment of input DNA with UV doses up to 100 Jm^(-2) did not have a significant effect on recombination, on the other hand, treating the recipient EJ cells with UV light at a dose of 1 Jm^(-2) prior to transfection resulted in a two fold increase in recombination. The tumor promoter TPA treatment did not have an effect on recombination or transformation. We have studied the effect of heterologous inserts on homologous recombination and observed that as the length of insert increased, there was a decline in the frequency of recombination.
A Pan-Cancer Proteogenomic Atlas of PI3K/AKT/mTOR Pathway Alterations
Zhang, Yiqun,Kwok-Shing Ng, Patrick,Kucherlapati, Melanie,Chen, Fengju,Liu, Yuexin,Tsang, Yiu Huen,de Velasco, Guillermo,Jeong, Kang Jin,Akbani, Rehan,Hadjipanayis, Angela,Pantazi, Angeliki,Bristow, C Elsevier Science B.V., Amsterdam 2017 CANCER CELL Vol.31 No.6
In vivo wide-area cellular imaging by side-view endomicroscopy
Kim, Pilhan,Chung, Euiheon,Yamashita, Hiroshi,Hung, Kenneth E,Mizoguchi, Atsushi,Kucherlapati, Raju,Fukumura, Dai,Jain, Rakesh K,Yun, Seok H Nature Publishing Group 2010 NATURE METHODS Vol.7 No.4
In vivo imaging of small animals offers several possibilities for studying normal and disease biology, but visualizing organs with single-cell resolution is challenging. We describe rotational side-view confocal endomicroscopy, which enables cellular imaging of gastrointestinal and respiratory tracts in mice and may be extensible to imaging organ parenchyma such as cerebral cortex. We monitored cell infiltration, vascular changes and tumor progression during inflammation and tumorigenesis in colon over several months.
Single Chromosome Based Physical Mapping of Human Chromosome 12
Lau, Stephanie,Marondel, Lvone,Yoon Kim, Sung-Joo,Chang, Joan,Montgomery, Kate,Renault, Beatrice,Kucherlapati, Raiu 가톨릭 의과학연구원 1997 가톨릭 의과학연구원 국제학술대회 Vol.1 No.-
Human chromosome 12 constitutes approximately 4.5% of the human genome and has an estimated size of 130 megabases. We have initiated an effort to construct an integrated high resolution physical, genetic, and cytogenetic map of this chromosome using an STS-content based strategy with overlapping YACs. The foundation for this map is the first generation physical map presented by Cohen et al.
Analyzing Somatic Genome Rearrangements in Human Cancers by Using Whole-Exome Sequencing
Yang, L.,Lee, M.S.,Lu, H.,Oh, D.Y.,Kim, Y.,Park, D.,Park, G.,Ren, X.,Bristow, Christopher A.,Haseley, Psalm S.,Lee, S.,Pantazi, A.,Kucherlapati, R.,Park, W.Y.,Scott, Kenneth L.,Choi, Y.L.,Park, Peter University of Chicago Press [etc.] 2016 American journal of human genetics Vol.98 No.5
<P>Although exome sequencing data are generated primarily to detect single-nucleotide variants and indels, they can also be used to identify a subset of genomic rearrangements whose breakpoints are located in or near exons. Using >4,600 tumor and normal pairs across 15 cancer types, we identified over 9,000 high confidence somatic rearrangements, including a large number of gene fusions. We find that the 50 fusion partners of functional fusions are often housekeeping genes, whereas the 30 fusion partners are enriched in tyrosine kinases. We establish the oncogenic potential of ROR1-DNAJC6 and CEP85L-ROS1 fusions by showing that they can promote cell proliferation in vitro and tumor formation in vivo. Furthermore, we found that similar to 4% of the samples have massively rearranged chromosomes, many of which are associated with upregulation of oncogenes such as ERBB2 and TERT. Although the sensitivity of detecting structural alterations from exomes is considerably lower than that from whole genomes, this approach will be fruitful for the multitude of exomes that have been and will be generated, both in cancer and in other diseases.</P>