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      • Types of indigenous vegetables consumed, preparation, preferences and perceived benefits in Ghana

        Atuna Richard A.,Djah Jemima,Achaglinkame Matthew A.,Bakker Sanne,Dari Linda,Osei-Kwarteng Mildred,Mahunu Gustav K.,Koomen Irene,Amagloh Francis K. 한국식품연구원 2022 Journal of Ethnic Foods Vol.9 No.-

        Indigenous vegetables (IVs) are rich sources of essential nutrients, particularly vitamins and minerals, and other non-nutritive phytochemicals. IVs play a critical role in the food culture of the Ghanaian people. Despite their importance, they have over the years been mostly associated with the resource-poor. In this study, the types of IVs, preparation preferences, frequency of consumption and some perceived benefits ascribed by some Ghanaian consumers ( n  = 1393) were investigated in a cross-sectional survey. Descriptive analysis and a chi-square test of independence were conducted to summarise and determine the relationship between gender, age and consumption frequency of the IVs. The study identified okra ( Abelmoschus esculentus ), cocoyam (Xanthosoma sagittifolium) leaves, jute mallow (Corchorus olitorius) and garden egg (Solanum melongena) as the popular IVs consumed by respondents. Among these, about 13.3% of women and 15.3% of men indicated they consume okra. Eight per cent of the women respondents and 6.7% of the men consumed jute mallow. Almost 57% of the respondents said the IVs were used to prepare stew and soup. More than a third (39%) of the respondents indicated that they consumed the IVs either daily or more than once a week. Women respondents’ frequency of IVs consumption was significantly different [X2(4,n= 1393) = 30.11,p= 0.000] from the men. The frequency of consumption of IVs for the elderly was significantly higher [X2(12,n= 1393) = 30.53,p= 0.002] as compared to the younger respondents. The main perceived benefits of IVs were nutrition- and health-related. The major barriers to the consumption of IVs were cost, non-availability, palatability and cultural. The IVs were largely wet-cooked and consumed as stews or sauces and soups. These findings form good bases for further empirical studies on the nutritional and other health-benefiting attributes of the specific IVs found in this study for sustainable promotion and utilisation in Ghana.

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        Targeted Peptide Measurements in Biology and Medicine: Best Practices for Mass Spectrometry-based Assay Development Using a Fit-for-Purpose Approach

        Carr, Steven A.,Abbatiello, Susan E.,Ackermann, Bradley L.,Borchers, Christoph,Domon, Bruno,Deutsch, Eric W.,Grant, Russell P.,Hoofnagle, Andrew N.,,ttenhain, Ruth,Koomen, John M.,Liebler, Danie The American Society for Biochemistry and Molecula 2014 Molecular and Cellular Proteomics Vol.13 No.3

        <P>Adoption of targeted mass spectrometry (MS) approaches such as multiple reaction monitoring (MRM) to study biological and biomedical questions is well underway in the proteomics community. Successful application depends on the ability to generate reliable assays that uniquely and confidently identify target peptides in a sample. Unfortunately, there is a wide range of criteria being applied to say that an assay has been successfully developed. There is no consensus on what criteria are acceptable and little understanding of the impact of variable criteria on the quality of the results generated. Publications describing targeted MS assays for peptides frequently do not contain sufficient information for readers to establish confidence that the tests work as intended or to be able to apply the tests described in their own labs. Guidance must be developed so that targeted MS assays with established performance can be made widely distributed and applied by many labs worldwide. To begin to address the problems and their solutions, a workshop was held at the National Institutes of Health with representatives from the multiple communities developing and employing targeted MS assays. Participants discussed the analytical goals of their experiments and the experimental evidence needed to establish that the assays they develop work as intended and are achieving the required levels of performance. Using this “fit-for-purpose” approach, the group defined three tiers of assays distinguished by their performance and extent of analytical characterization. Computational and statistical tools useful for the analysis of targeted MS results were described. Participants also detailed the information that authors need to provide in their manuscripts to enable reviewers and readers to clearly understand what procedures were performed and to evaluate the reliability of the peptide or protein quantification measurements reported. This paper presents a summary of the meeting and recommendations.</P>

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