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李炳基,高正愛,殷鍾旋 全北大學校 1987 論文集 Vol.29 No.-
In order to investigate the effects fo anther stage, optimal basic media, kinds and concentrations of plant growth regulators, sucrose concentrations for callus and embryo formation, pollen dimorphism and low temperature pretreatment of floral bud on the emergence of embryogenic S pollen of Anemone coronaria in vivo or in vitro, anthers of Anemone coronaria were cultured in Murashige & Skoog's(M&S) and Bourgin & Nitsch's(H) basic media with the combination of auxins and cytodinins. The measurement of anther length could be reliable criterion in determining the optimal anther stages, while floral bud length and anther color could not be a reliable criterion because same sized floral buds showed various degree of microspore development. M&S basic media was more effective on callus formation than H basic media. The addition of plant growth regulators of NAA and BA with M&S basic media was effective on callus formation than 2,4-D and kinetin. The best results was obtained from the combination of 0.5mg/l NAA and 2.0 mg/l BA. The best response of calus formation was obtained from 3% sucrose concentration on M&S basic medium supplemented with 0.5mg/l NAA and 2.0mg/l BA. B type of S pollen was formed on each anther stages except anther stage I in vitro. Callus formation was effective just before or just after at first pollen mitosis, while callus formation was less effective at tetrad or mature pollen staes. Low temperature pretreatment on the first pollen mitosis anthers with 1℃ or 5℃ for 10 days induced above 25% of S pollen. Embryogenic S pollen were divided mostly into A type at 10℃ for 10 days pretreatment and rate of B type pollen was appeared 4.3% which was 5 times more than control. Callus formation was effective at 10℃ for 10 days pretreatment.
대추나무(Zizyphus jujuba Miller) 葯培養時 低溫 및 植物生長 調節物質處理가 葯分化 및 Callus發生에 미치는 影響
吳成都,高正愛 全北大學校 1991 論文集 Vol.33 No.-
대추나무(Zizyphus jujuba Miller)의 葯培養에 있어서 置床適基와 生長調節物質의 添加量, 異常花粉現象, 低溫處理 등이 callus形成에 미치는 영향을 규명하기 위하여 MS基本培地에 2,4-D, NAA 및 kinetin을 몇가지 濃度로 組合하여 低溫處理한 葯을 培養하였던 바 얻어진 결과는 다음과 같다. 1. 葯을 置床前 低溫處理하므로서 異常花粉의 比率이 증가하였는데 무처리구의 10.4%인데 비해 1℃, 3일처리구에서는 59.3%로 增加하였다. 2. 低溫處理한 葯을 培養했을 때 callus形成에는 5℃ 3일 處理區가 가장 양호하였다. 3. 低溫處理한 葯을 培養 후 60일 만에 花粉의 狀態를 調査하였던 바 低溫處理는 異常花粉을 增加시키고 退化花粉을 有意하게 減少시켰다. 4. Callus形成에 미치는 植物生長調節物質의 影響은 2,4-D, NAA, kinetin 2.0mg/l의 高濃度에서 가장 양호하였다. 5. 器官分化를 위하여 各種 植物生長調節物質을 첨가한 배지에 繼代培養하였던 바 kinetin 高濃度添加區에서 callus는 왕성하게 發生하였으나 모든 處理區에서 器官分化는 일어나지 않았다. A study was carried out to investigate the various optimal conditions for in vitro anther culture of Chinese jujube (Zizyphus jujuba Miller). Optimal anther stage, with low temperature pretreatment on flower buds, pollen dimorphism and composition of plant growth regulators added on medium were investigated for successful production of microspore-originated callus. The appearance rate of S pollen was 10.4% in vitro, while that with low temperature pretreatment for 3 days at 5℃ showed the best callus induction after 390 days culture. Low temperature treatment enhanced highly significantly the rate of S pollen appearance but reduced the degenerated pollen appearance in vitro in 60 days after treatment. High concentration of plant growth regulators (2,4-D, NAA, kinetin : 2㎎/l each) supplemented on MS basal medium was most effective on callus induction of originated anther. In the differentiation medium the high concentration of kinetin was relatively effective on callus induction, but there was no organ differentiation in any treatment.
국내산 과일 및 채소 추출물을 이용한 체중 조절 관련 기능성 식품소재 탐색
김인혜;김종배;조강진;황유진;황경아;고정숙;엄애선 한양대학교 2011 韓國 生活 科學 硏究 Vol.31 No.1
Obesity can be defined as a disorder of lipid metabolism and the control of this process is regulated by enzymes involved in lipid metabolic pathway. Obesity is also characterized increases in the number and size of adipocyte differentiated from fibroblastic pre-adipocytes in adipose tissue. The aim of this study was to screen ethanol extracts of 21 fruits and vegetables cultivated in Republic of Korea for identifying natural sources of lipase inhibitor and to investigate the effects of several extracts resulting in suppression of triglyceride (TG) accumulation in 3T3-L1 cells. Among the tested extracts, Diospyros kaki L. showed the highest contents of total sugar and the content of total phenolic compounds was detected 17 species while there was not detected in Ixeris dentate root and Allium fistulosum C. etc. We were screened 26 extracts of fruits and vegetables for pancreatic lipase inhibitory activity compared with orlistat. The strongest lipase inhibitory activities were shown by extract of Allium sativum L. stem, Allium sativum L. bulb, Apteryx australis, Diospyros kaki L. Malus Pumila, Perilla frutescens and Prunus mume regardless of concentrations treated. Eight extracts including Brassica juncea var. integrilolia had no effect on lipase inhibition. During the differentiation of 3T3-L1 preadipocyte to mature adipocyte, the cells were treated with carrier or 7 extracts(1, 5 and 10 ㎍/ml), which was shown to highest lipase inhibitory effects. We were quantified lipid contents secreted mature adipocytes and stained with Oil Red O. All cells treated with the Apteryx australis and Prunus mume significantly decreased TG contents than the control. These findings suggested that the screened fruit and vegetable could be a promising source as the effective and safe lipase inhibitors for isolating bioactive ingredients and developing functional foods.