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- 저자 : Kisato Kusano (검색결과 1 건)
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Naoyuki Moriya,Yukiko Moriya,Hideo Nomura,Kisato Kusano,Yukoh Asada,Hirofumi Uchiyama,Enoch Y. Park,Mitsuyasu Okabe 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.6
β-(1→3)-D-glucans with β-(1→6)-glycosidiclinked branches are known to be immune activation agentsand are incorporated in anti-cancer drugs and healthpromotingsupplements. β-Glucan concentration was 9.2 g/Lin a 200-L pilot scale fermentor using mutant strainAureobasidium pullulans M-2 from an imperfect fungalstrain belonging to A. pullulans M-1. The culture broth ofA. pullulans M-2 had a faint yellow color, whereas that ofthe wild-type had an intense dark green color caused by theaccumulation of melanin-like pigments. β-Glucan producedby A. pullulans M-2 was identified as a polysaccharide ofD-glucose monomers linked by β-(1→3, 1→6)-glycosidicbonds through GC/MS and NMR analysis. When aconventional medium was used in the culture of A. pullulansM-2 in a 3-L jar fermentor, β-glucan concentration was1.4-fold that produced by the wild-type. However, when amedium optimized by statistical experimental design wasused with dissolved oxygen at 10%, the β-glucan concentrationwas 9.9 g/L with a yield of 0.52 (g β-glucan/g consumedsucrose), 2.9-fold that of the wild-type. This level ofproductivity was reproduced when the fermentation wasscaled up 200-L. The industrial production of high β-glucan without melanin-like pigments is highly expected,as a health-promoting supplement or functional food.
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